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| 肥胖型多囊卵巢综合征患者外周血淋巴细胞miR-27a、miR-141与PI3K/AKT信号通路的相关性分析 |
| Correlation Analysis of miR-27a, miR-141 and PI3K/AKT Signaling Pathway in Peripheral Blood Lymphocytes of Obese Patients With Polycystic Ovary Syndrome |
| 投稿时间：2024-07-02 修订日期：2024-07-02 |
| DOI： |
| 中文关键词: 肥胖型多囊卵巢综合征 miR-27a miR-141 PI3K/Akt信号通路 |
| 英文关键词: Obese type of polycystic ovary syndrome miR-27a miR-141 PI3K/Akt signaling pathway |
| 基金项目:国家自然科学基金青年项目（81502451） |
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| 中文摘要: |
| 目的 探讨肥胖型多囊卵巢综合征（PCOS）患者外周血淋巴细胞微小核糖核酸（MicroRNA，miR）-27a、miR-141与磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（AKT）信号通路的相关性。方法 选取2021年1月至2023年10月同济大学附属第十人民医院收治的110例PCOS患者，根据患者体质量指数（BMI）分为非肥胖组（40例）和肥胖组（70例）。对外周血淋巴细胞miR-27a、miR-141与外周血PI3K mRNA、AKT mRNA表达水平，采用实时荧光定量 PCR 技术检测，其相关性采用Pearson法分析。结果 对比非肥胖组，肥胖组PCOS患者外周血淋巴细胞miR-27a、miR-141的表达水平更高，但外周血PI3K mRNA、AKT mRNA的表达水平更低（P＜0.05）。PCOS患者外周血淋巴细胞miR-27a、miR-141表达与外周血 PI3K mRNA、AKT mRNA表达呈负相关（P＜0.05）。结论 肥胖型PCOS患者外周血淋巴细胞miR-27a、miR-141表达增高，外周血PI3K mRNA、AKT mRNA表达降低。miR-27a、miR-141高表达和与PCOS患者PI3K/Akt信号通路受到抑制有关。 |
| 英文摘要: |
| Objective:To investigate the correlation between microRNA (miR) -27a, miR-141 and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signa/ing pathway in peripheral blood lymphocytes of obese patients with polycystic ovary syndrome (PCOS). Methods:110 PCOS patients admitted to Tenth People''s Hospital of Tongji University from January 2021 to October 2023 were selected,and patients were divided into non-obese group (40 cases) and obese group (70 cases) according to their body mass index(BMI).The expression levels of miR-27a and miR-141 in peripheral blood lymphocytes and PI3K mRNA and AKT mRNA in peripheral blood were detected by real-time fluorescence quantitative PCR, and the correlation were analyzed by Pearson method. Results:Compared with non-obese group, the expression levels of miR-27a and miR-141 in peripheral blood lymphocytes of PCOS patients in obese group were higher, the expression levels of PI3K mRNA and AKT mRNA in peripheral blood were lower (P<0.05). The expression of miR-27a and miR-141 in peripheral blood lymphocytes of PCOS patients was negatively correlated with the expression of PI3K mRNA and AKT mRNA in peripheral blood(P<0.05). Conclusion:The expression of miR-27a and miR-141 in peripheral blood lymphocytes of obese PCOS patients increased, and the expression of PI3K mRNA and AKT mRNA in peripheral blood decreased. The high expression of miR-27a and miR-141 are related to the inhibition of PI3K/Akt signaling pathway in PCOS patients. |
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