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| 免疫缺陷小鼠大肠埃希菌感染致死案例的深入分析与启示 |
| In-depth analysis and revelation of fatal cases of Escherichia coli infection in immunodeficient mice |
| 投稿时间：2024-07-02 修订日期：2024-07-15 |
| DOI： |
| 中文关键词: 大肠埃希菌 免疫缺陷 小鼠 致病菌 胃肠功能紊乱 |
| 英文关键词: Escherichia coli Immune deficiency Mice Pathogenic bacteria Gastrointestinal dysfunction |
| 基金项目: |
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| 中文摘要: |
| 目的：本研究旨在探究实验室饲养的免疫缺陷SPF雌性小鼠在哺乳期异常死亡的原因。方法：为了深入了解死亡原因,我们对死亡的三只雌鼠和幼崽及一只对照哨兵鼠进行了详细的病理解剖,并对肝脏、脾脏、肠道及肛门进行了细菌培养及鉴定；将死亡小鼠及同笼架哨兵鼠的心脏、肺部、肝脏、脾脏、胃部及肠道组织进行病理切片及HE染色。结果：雌鼠解剖发现胃内大量的饲料积累,肠道基本为排空状态,内无粪便,肝脏、脾脏及肠道无异常表现。死亡雌鼠的心脏、肺部、肝脏及脾脏都有炎症细胞浸润；细菌培养结果显示异常死亡小鼠肝脏、脾脏、肠道及肛门处有细菌生长,对照哨兵鼠肝脏及脾脏无细菌生长。经时间飞行质谱仪鉴定异常死亡小鼠肝脏、脾脏、肠道及肛门处主要致病菌为大肠埃希菌,哨兵鼠肠道主要细菌为缓慢葡萄球菌及加氏乳杆菌,肛门处为土壤短芽孢杆菌。结论：研究表明,该案例为小鼠胃肠功能紊乱导致内源性感染大肠埃希菌引起的小鼠死亡,提示在免疫缺陷小鼠饲养过程中需要密切观察粪便的变化,提前预防。 |
| 英文摘要: |
| Objective: This study looked into the causes of aberrant death in immunodeficient SPF female mice during lactation. Methods: Three female mice and their pups, as well as a control sentry mouse, were thoroughly dissected to determine the cause of death. The liver, spleen, intestine, and anus were cultured and identified. The heart, lung, liver, spleen, stomach, and intestinal tissues of both dead and sentry mice in the same cage were pathologically cut and stained with HE. Results: The stomach had accumulated a large amount of feed, the intestines were empty, and the liver, spleen, and intestines were free of stool. The heart, lungs, liver and spleen of the dead female mice were infiltrated with inflammatory cells. The bacterial culture findings indicated that the abnormal dead mice had bacterial growth in the liver, spleen, intestine, and anus. In contrast, the control mice had no bacterial growth in the liver and spleen. Escherichia coli was identified as the main pathogenic bacteria in liver, spleen, intestine and anus of abnormal dead mice by time-flight mass spectrometer. The main bacteria in the intestine of sentinel mice were staphylococcus lentus and Lactobacillus gasseri, and the main bacteria in the anus were Brevibacillus agri strain. Conclusions: The study showed that this case was the death of mice caused by endogenous Escherichia coli infection caused by gastrointestinal dysfunction of mice, suggesting that the changes of feces should be closely observed and prevented in advance during the feeding of immunodeficient mice. |
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