| Objective: To investigate the regulatory effect of high estrogen exposure on placental damage and immune function in pregnant rats with gestational hypertension (GH). Methods: GH rat model was constructed, and rats were randomly divided into control group (Control group), GH group, low-dose estrogen group (L-estrogen group), medium-dose estrogen group (M-estrogen group) and high-dose estrogen group (H-estrogen group).The systolic pressure, diastolic pressure, 24h urine protein, follicle stimulating hormone (FSH), estradiol (E2) and luteinizing hormone (LH) contents of pregnant rats were detected. The pathological changes of placental tissue in pregnant rats were observed by hematoxylin eosin (HE) staining. The ultrastructure of placental trophoblast cells in pregnant rats was observed by transmission microscope. The proportion of helper T cells (Th) 17/regulatory T cells (Treg) was detected by flow cytometry. The levels of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin (IL) -6 and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA). Results The number of villi in GH group was decreased, the nucleus of trophoblast cells was pyknosis, systolic blood pressure, diastolic blood pressure, 24h urine protein, Th17 cell ratio, Th17/Treg ratio, IFN-γ, TNF-α, IL-6 and IL-17 were significantly increased, and the proportion of FSH, E2, LH and Treg cells was significantly decreased (P<0.05). Compared with GH group, the placental tissue damage and nuclear pyknosis of trophoblast cells were reduced in L-estrogen group, M-estrogen group and H-estrogen group, systolic blood pressure, diastolic blood pressure, 24h urine protein, Th17 cell ratio, Th17/Treg ratio, IFN-γ, TNF-α, IL-6 and IL-17 levels were significantly decreased, and the proportion of FSH, E2, LH and Treg cells was significantly increased in a dose-dependent manner (P<0.05). Conclusion: Estrogen exposure can reduce the blood pressure of GH rats, improve placental damage and immune function in GH pregnant rats, and the intervention effect of high-dose estrogen is better. |
