| 周 磊,史承勇,范茂丹,宋 双,斯银平,黄骏咏,秦 荣.高压氧对TBI大鼠脑组织中氧化因子表达情况和Nrf2 通路的影响[J].,2024,(7):1240-1243 |
| 高压氧对TBI大鼠脑组织中氧化因子表达情况和Nrf2 通路的影响 |
| Effects of Hyperbaric Oxygen on Oxidative Factor Expression and Nrf2 Signaling Pathway in Brain of Rats with Traumatic Brain Injury |
| 投稿时间：2023-06-05 修订日期：2023-06-28 |
| DOI：10.13241/j.cnki.pmb.2024.07.007 |
| 中文关键词: 创伤性脑损伤 高压氧 氧化应激反应 核因子E2相关因子2 血红素氧合酶 |
| 英文关键词: Traumatic brain injury Hyperbaric oxygen Oxidative stress NF-E2-related factor 2 Heme oxygenase-1 |
| 基金项目:中国人民解放军联勤保障部队第908医院专项课题（YGKTLC2016002）；江西省卫生健康委科技计划项目（202311379） |
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| 中文摘要: |
| 摘要 目的：研究高压氧（Hyperbaric oxygen，HBO）治疗对创伤性脑损伤（Traumatic brain injury，TBI）大鼠脑组织中氧化因子表达和核因子E2相关因子2（NF-E2-related factor 2，Nrf2）/血红素氧合酶（Heme oxygenase-1，HO-1）信号通路的影响。方法：将72只雄性SD大鼠随机分为假手术组（SHAM组）、创伤性脑损伤组（TBI组）、高压氧治疗组（HBO组），每组24只；再按TBI造模后3天、7天将每组大鼠分为3d、7d两个亚组，每个亚组12只。分别于实验第3 d、7 d对各组大鼠进行改良神经损伤严重程度评分（Modified neurological severity score，mNSS）；使用酶活试剂盒检测脑组织中超氧化物歧化酶（Superoxide dismutase，SOD）、丙二醛（Malondialdehyde，MDA）、过氧化氢酶（Catalase，CAT）表达水平；Western blot法检测脑组织中Nrf2、HO-1蛋白的表达水平。结果：TBI组、HBO组大鼠神经功能在实验后3 d、7 d均较SHAM组出现不同程度下降(P<0.05），HBO组大鼠神经功能较TBI组改善(P<0.05）。在实验后3 d、7 d，TBI、HBO组大鼠脑组织中SOD、CAT表达水平较SHAM组降低(P<0.05），MDA表达水平较SHAM组升高(P<0.05）；同时，HBO组大鼠脑组织中SOD、CAT表达水平较TBI组升高(P<0.05），MDA表达水平较TBI组降低(P<0.05）。Western blot检测发现，在实验后3 d，TBI组、HBO组大鼠脑组织中Nrf2、HO-1蛋白的表达水平均较SHAM组升高(P<0.05），且HBO组Nrf2、HO-1蛋白的表达水平均较TBI组升高(P<0.05)。但在实验后7 d，TBI组大鼠脑组织中Nrf2、HO-1蛋白的表达水平均较SHAM组下降(P<0.05），HBO组大鼠脑组织中Nrf2、HO-1蛋白的表达水平较SHAM组、TBI组均升高(P<0.05）。结论：大鼠在TBI后第3 d、7 d出现不同程度的神经功能损伤，高压氧治疗能够通过调节Nrf2/HO-1信号通路，调控下游SOD、MDA、CAT表达水平，抑制氧化应激反应，起到改善神经功能的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of hyperbaric oxygen treatment on the expression of oxidative factors and nuclear factor E2 related factor 2 / heme oxygenase-1 signaling pathway in the brain of traumatic brain injury rats. Methods: 72 male SD rats were randomly divided into sham operation group, traumatic brain injury group and hyperbaric oxygen treatment group, with 24 rats in each group. The rats in each group were divided into 3 d and 7 d subgroups, with 12 rats in each group. The modified neurological severity score was performed on the 3 d and 7 d. The expression levels of superoxide dismutase, malondialdehyde and catalase in brain tissue were detected by enzyme activity kits. The expression levels of Nrf2 and HO-1 proteins in brain tissue were detected by Western blot. Results: The neurological function of rats in TBI group and HBO group decreased in varying degrees compared with rats in SHAM group at 3 d and 7 d after the experiment (P<0.05), and the neurological function of rats in HBO group was improved compared with TBI group (P<0.05). The expression levels of SOD and CAT of rats in TBI and HBO groups were lower than those in SHAM group (P<0.05), and the expression level of MDA was higher than that in SHAM group (P<0.05); At the same time, the expression levels of SOD and CAT of rats in HBO group were higher than those in TBI group(P<0.05), and the expression level of MDA was lower than that in TBI group (P<0.05). Western blot showed that three days after the experiment, the expression levels of Nrf2 and HO-1 protein of rats in TBI group and HBO group were higher than those in SHAM group (P<0.05), and the expression levels of Nrf2 and HO-1 protein in HBO group were higher than those in TBI group (P<0.05). However, on the 7th day after the experiment, the expression levels of Nrf2 and HO-1 protein of rats in TBI group were lower than those in SHAM group(P<0.05), and the expression levels of Nrf2 and HO-1 protein in HBO group were higher than those in SHAM group and TBI group (P<0.05). Conclusion: Rats have different degrees of neurological damage on the 3 d and 7 d after TBI. Hyperbaric oxygen treatment can improve neurological function by regulating Nrf2 / HO-1 signal pathway, which can regulate the expression levels of SOD, MDA , CAT and inhibit oxidative stress response. |
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