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| DRAM2通过p53和自噬影响非小细胞肺癌的增殖和迁移 |
| DRAM2 affects proliferation and migration of non-small cell lung cancer through p53 and autophagy |
| 投稿时间：2024-06-14 修订日期：2024-06-14 |
| DOI： |
| 中文关键词: 非小细胞肺癌 DRAM2 p53 自噬 |
| 英文关键词: Non-small cell lung cancer DRAM2 P53 Autophagy |
| 基金项目:新疆维吾尔自治区自然科学基金（编号：2020D01C175）；新疆维吾尔自治区重点研发计划项目支持（编号：2022B03019-1-1） |
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| 中文摘要: |
| 目的：探讨非小细胞肺癌中DRAM2通过p53和自噬对细胞增殖和迁移的影响机制。方法：在前期实验证实DRAM2能促进非小细胞肺癌增殖和迁移并抑制p53表达的基础上 ，本实验进一步建立慢病毒稳定敲除DRAM2的细胞系，应用免疫荧光和western blot检测DRAM2敲除后自噬标志性分子LC3 和 p62 的表达，随后在敲除DRAM2的细胞中使用自噬激活后检测细胞细胞增殖和迁移，最后在敲除DRAM2的同时敲除p53表达后再次检测自噬及细胞增殖和迁移的变化。结果：1.敲低DRAM2抑制非小细胞肺癌自噬；2.敲低DRAM2可通过抑制自噬从而抑制非小细胞肺癌增殖和迁移；3.敲低DRAM2同时敲低p53将解除自噬的抑制进而恢复非小细胞肺癌增殖和迁移。结论：敲低DRAM2可上调p53表达并抑制自噬进而抑制非小细胞肺癌的细胞增殖和迁移。 |
| 英文摘要: |
| Objective: Explore the mechanism by which DRAM2 affects the proliferation and migration of non-small cell lung cancer cells through p53 and autophagy. Method:On the basis of previous experiments confirming that DRAM2 can promote the proliferation and migration of non-small cell lung cancer and inhibit p53 expression, this experiment further established a slow virus stable knockout cell line of DRAM2. Immunofluorescence and Western blot were used to detect the expression of autophagy markers LC3 and p62 after DRAM2 knockout. Subsequently, autophagy activation was used to detect cell proliferation and migration in DRAM2 knockout cells. Finally, changes in autophagy and cell proliferation and migration were detected again after knocking out p53 expression while DRAM2 was knocked out. Result: 1. Knocking down DRAM2 inhibits autophagy in non-small cell lung cancer; 2. Knocking down DRAM2 can inhibit the proliferation and migration of non-small cell lung cancer by inhibiting autophagy; Knocking down DRAM2 while knocking down p53 will relieve the inhibition of autophagy and restore the proliferation and migration of non-small cell lung cancer. Conclusion: Knocking down DRAM2 can upregulate p53 expression and inhibit autophagy, thereby inhibiting cell proliferation and migration in non-small cell lung cancer. |
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