| 王放明,乔新伟,陈玖玲,刘红菊,赵 峰.非小细胞肺癌组织PAK4、PAK5蛋白表达与上皮-间质转化、临床病理特征和预后的关系分析[J].,2024,(2):253-257 |
| 非小细胞肺癌组织PAK4、PAK5蛋白表达与上皮-间质转化、临床病理特征和预后的关系分析 |
| Relationship between PAK4, PAK5 Protein Expression and Epithelial-mesenchymal Transition, Clinicopathological Features and Prognosis in Non-small Cell Lung Cancer |
| 投稿时间：2023-06-20 修订日期：2023-07-15 |
| DOI：10.13241/j.cnki.pmb.2024.02.009 |
| 中文关键词: 非小细胞肺癌 PAK4 PAK5 上皮-间质转化 临床病理特征 预后 |
| 英文关键词: Non-small cell lung cancer PAK4 PAK5 Epithelial-mesenchymal transition Clinicopathological features Prognosis |
| 基金项目:湖北省自然科学基金项目（2018CKB907） |
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| 中文摘要: |
| 摘要 目的：探讨非小细胞肺癌（NSCLC）组织p21激活激酶（PAK）4、PAK5蛋白表达与上皮-间质转化（EMT）、临床病理特征和预后的关系。方法：选取2018年1月～2019年12月我院收治的100例NSCLC患者，收集手术切除的癌组织和癌旁组织标本，采用免疫组化法检测NSCLC组织和癌旁组织中PAK4、PAK5和EMT相关蛋白[E-钙粘蛋白（E-Cad）、N-钙粘蛋白（N-Cad）和波形蛋白（VIM）]表达。分析PAK4、PAK5蛋白表达与NSCLC患者病理特征的关系和与EMT相关蛋白的相关性。根据NSCLC组织中PAK4、PAK5表达分为阳性/阴性表达组，采用K-M法绘制PAK4、PAK5阳性/阴性表达NSCLC患者的生存曲线，多因素Cox回归分析NSCLC患者死亡的影响因素。结果：与癌旁组织相比，NSCLC组织中PAK4、PAK5、N-Cad、VIM蛋白阳性表达率升高，E-Cad蛋白阳性表达率降低（P＜0.05）。二列相关性分析显示，NSCLC组织PAK4、PAK5与E-Cad蛋白阳性表达率呈负相关，与N-Cad、VIM蛋白阳性表达率呈正相关（P均＜0.001）。不同分化程度、TNM分期、淋巴结转移NSCLC患者PAK4、PAK5蛋白阳性表达率比较，差异有统计学意义（P＜0.05）。100例NSCLC患者3年总生存率为56.00%（56/100）。K-M生存曲线分析显示，PAK4、PAK5阳性表达组总生存率低于阴性表达组（P＜0.05）。多因素Cox回归分析显示，低分化、TNM分期为ⅢA期、淋巴结转移和PAK4、PAK5蛋白阳性表达为NSCLC患者死亡的独立危险因素（P＜0.05）。结论：NSCLC组织PAK4、PAK5蛋白表达升高，与EMT、分化程度、TNM分期、淋巴结转移和预后有关，可能成为NSCLC诊治的新靶点。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between p21-activated kinase (PAK) 4, PAK5 protein expression and epithelial-mesenchymal transition (EMT), clinicopathological features and prognosis in non-small cell lung cancer (NSCLC). Methods: 100 NSCLC patients who were admitted to our hospital from January 2018 to December 2019 were selected, collect surgically excised cancer tissue and adjacent tissue samples, the expressions of PAK4, PAK5 and EMT-related proteins [E-cadherin (E-Cad), N-cadherin (N-Cad) and vimentin (VIM)] in NSCLC tissues and tissues adjacent to cancer were detected by immunohistochemistry. The relationship between the expression of PAK4 and PAK5 proteins and the pathological features of NSCLC patients and the correlation with EMT-related proteins were analyzed. Patients were divided into positive/negative expression group according to the expression of PAK4 and PAK5 in NSCLC tissues. Survival curves of NSCLC patients with positive/negative expression of PAK4 and PAK5 were drawn by K-M method, and the influencing factors of death in NSCLC patients were analyzed by multivariate Cox regression analysis. Results: Compared with tissues adjacent to cancer, positive expression rates of PAK4, PAK5, N-Cad and VIM proteins in NSCLC tissues were increased, positive expression rate of E-Cad protein was decreased (P<0.05). Two column correlation analysis showed that, PAK4 and PAK5 were negatively correlated with the positive expression rate of E-Cad protein in NSCLC tissues, there was a positive correlation between the positive expression rate of N-Cad and VIM protein (all P<0.001). The positive expression rates of PAK4 and PAK5 protein in NSCLC patients with different differentiation degree, TNM stage and lymph node metastasis were compared, the difference was statistically significant (P<0.05). The 3-year overall survival rate of 100 NSCLC patients was 56.00 % (56/100). K-M survival curve analysis showed that, overall survival rate of PAK4 and PAK5 positive expression group was lower than that of negative expression group (P<0.05). Multivariate Cox regression analysis showed that, poor differentiation, TNM stage IIIA, lymph node metastasis and positive expression of PAK4 and PAK5 proteins were independent risk factors for death in NSCLC patients (P<0.05). Conclusion: The expression of PAK4 and PAK5 proteins in NSCLC tissue increases, which is relate to EMT, differentiation, TNM stage, lymph node metastasis and prognosis, may become a new target for the diagnosis and treatment of NSCLC. |
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