| 陈宗浩,李 恒,杨玉平,喻才元,程昌进.食管癌组织环指蛋白2、环指蛋白6的表达与上皮-间质转化和预后的关系分析[J].,2024,(1):189-193 |
| 食管癌组织环指蛋白2、环指蛋白6的表达与上皮-间质转化和预后的关系分析 |
| Analysis of the Relationship between the Expression of Ring Finger Protein 2 and Ring Finger Protein 6 in Esophageal Carcinoma Tissues and Epithelial-Mesenchymal Transformation and Prognosis |
| 投稿时间：2023-06-05 修订日期：2023-06-28 |
| DOI：10.13241/j.cnki.pmb.2024.01.038 |
| 中文关键词: 食管癌 环指蛋白2 环指蛋白6 临床病理特征 上皮-间质转化 预后 |
| 英文关键词: Esophageal carcinoma Ring finger protein 2 Ring finger protein 6 Clinical pathological features Epithelial-mesenchymal transformation Prognosis |
| 基金项目:广东省自然科学基金项目（2017A030313546） |
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| 中文摘要: |
| 摘要 目的：探讨食管癌组织环指蛋白2（RNF2）、环指蛋白6（RNF6）的表达与上皮-间质转化（EMT）和预后的关系。方法：选择广东医科大学附属医院2017年2月至2020年2月收治的162例食管癌患者，取手术切除的癌组织和癌旁组织。采用免疫组化法检测RNF2、RNF6以及EMT标志蛋白[上皮钙黏附素（E-cadherin）、神经型钙黏蛋白（N-cadherin）Slug 和Snail]表达。Spearman相关性分析RNF2、RNF6与EMT标志蛋白的关系；分析食管癌组织RNF2、RNF6表达在不同临床病理特征中的差异；Kaplan-Meier生存曲线分析RNF2、RNF6表达与食管癌患者预后的关系；多因素Cox回归分析影响食管癌患者预后的因素。结果：食管癌组织RNF2、RNF6、N-cadherin、Slug和Snail蛋白阳性表达率高于癌旁组织，E-cadherin蛋白阳性表达率低于癌旁组织（P＜0.05）。食管癌组织RNF2、RNF6蛋白阳性表达率与N-cadherin、Slug和Snail蛋白阳性表达率呈正相关，与E-cadherin蛋白阳性表达率呈负相关（P＜0.05）；低度分化、TNM分IIIA期、肿瘤直径≥2 cm、淋巴结转移在食管癌组织中RNF2、RNF6蛋白阳性表达率高于无淋巴结转移、肿瘤直径＜2 cm，中高度分化、TNM分期I～II期食管癌组织（P＜0.05）；RNF2阳性表达患者3年OS率为47.17%，低于RNF2阴性表达患者的59.26% （P＜0.05），RNF6阳性表达患者3年OS率为47.06%，低于RNF6阴性表达患者的63.41%（P＜0.05）；多因素Cox回归分析显示TNM分期ⅢA期、淋巴结转移、RNF2阳性表达、RNF6阳性表达是食管癌患者预后的危险因素（P＜0.05）。结论：食管癌组织中RNF2、RNF6阳性表达率增加，且与肿瘤直径、分化程度、TNM分期、淋巴结转移以及低生存率有关，RNF2、RNF6可能通过EMT参与食管癌恶性进展过程。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression of ring finger protein 2 (RNF2) and ring finger protein 6 (RNF6) in esophageal carcinoma tissues and the epithelial-mesenchymal transformation (EMT) and prognosis. Methods: 162 patients with esophageal carcinoma admitted to Affiliated Hospital of Guangdong Medical University from February 2017 to February 2020 were selected, and the surgically removed carcinoma tissues and adjacent cancerous tissues were taken. Immunohistochemical staining was used to detect the expression of RNF2, RNF6, and EMT marker proteins[E-cadherin, N-cadherin Slug, and Snail]. The relationship between RNF2, RNF6, and EMT marker protein were analyzed by Spearman correlation analysis; the differences in RNF2 and RNF6 expression in different clinical pathological features of esophageal cancer tissue were analyzed; Kaplan-Meier survival curve was used to analyze the relationship between the expression of RNF2 and RNF6 and the prognosis of patients with esophageal carcinoma; Multivariate Cox regression was used to analyze the prognostic factors of patients with esophageal carcinoma. Results: The positive expression rates of RNF2, RNF6, N-cadherin, Slug and Snail in esophageal carcinoma tissue were higher than those in adjacent cancerous tissues, the positive expression rate of E-cadherin protein was lower than that in adjacent canceroustissues(P＜0.05). The positive expression rates of RNF2 and RNF6 proteins in esophageal carcinoma tissues were positively correlated with the positive expression rates of N-cadherin, Slug and Snail proteins,and negatively correlated with the positive expression rate of E-cadherin protein(P＜0.05). Poorly differentiated, TNM stage IIIA, tumor diameter ≥2 cm, lymph node metastasis in esophageal cancer tissue with higher positive expression rates of RNF2 and RNF6 proteins than without lymph node metastasis, tumor diameter<2 cm, medium to high differentiated, TNM stage I-II esophageal cancer tissue(P＜0.05). The 3-year OS rate in patients with positive expression of RNF2 was 47.17%, which was lower than 59.26% in patients with negative expression of RNF2(P＜0.05), the 3-year OS rate in patients with positive expression of RNF6 was 47.06%, which was lower than 63.41% in patients with negative expression of RNF6(P＜0.05). Multivariate Cox regression analysis showed that TNM stage IIIA, lymph node metastasis, positive expression of RNF2 and positive expression of RNF6 were risk factors for prognosis of patients with esophageal carcinoma(P＜0.05). Conclusion: The positive expression rates of RNF2 and RNF6 increased in esophageal carcinoma tissues, and they are related to tumor diameter, differentiation degree, TNM stage, lymph node metastasis, and low survival rate. RNF2 and RNF6 may participate in the malignant progression of esophageal carcinoma through EMT. |
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