| 余宏微,陈静文,贺美娟,范旭辉,王毅晖,王 悍.巨噬细胞膜仿生纳米铁颗粒制备及多形性胶质母细胞瘤MRI成像的初步实验研究[J].,2024,(1):6-11 |
| 巨噬细胞膜仿生纳米铁颗粒制备及多形性胶质母细胞瘤MRI成像的初步实验研究 |
| Experimental Study of Nanoparticles with Biomimetic Macrophage Membrane for MRI Imaging of Glioblastoma |
| 投稿时间：2023-07-04 修订日期：2023-07-30 |
| DOI：10.13241/j.cnki.pmb.2024.01.002 |
| 中文关键词: 仿生纳米颗粒 MRI成像 多形性胶质母细胞瘤 |
| 英文关键词: Biomimetic nanoparticles MRI imaging Glioblastoma multiforme |
| 基金项目:国家自然科学基金项目（82271969）；上海市教委重大项目（202101070002E00085）；上海市科学技术委员会项目（19411951403） |
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| 中文摘要: |
| 摘要 目的：探讨巨噬细胞膜仿生的纳米铁颗粒（Fe3O4 NCs@MM）对多形性胶质母细胞瘤MRI成像的研究。方法：制备巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM，利用动态光散射（Dynamic Light Scattering，DLS）和透射电子显微镜（Transmission Electron Microscope，TEM）对其水合动力学粒径、表面电势和形态进行表征。采用SDS-聚丙烯酰胺凝胶电泳（sodium dodecyl sulphate-polyacrylamide gel electrophoresis，SDS-PAGE）评价巨噬细胞膜的完整包覆；紫外可见光谱测定巨噬细胞膜仿生的纳米铁颗粒抗蛋白吸附能力。通过MRI成像系统，分析了含不同浓度的Fe元素（0.1-1.6 mM）的Fe3O4 NCs@MM在GSH存在或不存在时的T1弛豫效应。采用细胞增殖-毒性实验（Cell Counting Kit-8，CCK-8），测定巨噬细胞膜仿生纳米铁颗粒处理肿瘤细胞24 h后的细胞活性。尾静脉注射巨噬细胞膜仿生纳米铁颗粒至原位胶质母细胞瘤模型中，观察成像效果。结果：巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM的水合动力学粒径和表面电势分别为 286.5±7.6 nm和-20.7±3.5 mV，且在水溶液中分布均匀，具有较好的单分散性。包覆巨噬细胞膜的纳米铁颗粒具备抗蛋白吸附的能力。MRI成像显示，制备的巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM为GSH响应型MRI对比剂，具有较好的T1-加权磁共振成像效果，在尾静脉注射巨噬细胞膜的纳米铁颗粒0.5 h后，肿瘤部位的信号可见增强。结论：巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM可实现多形性胶质母细胞瘤的MRI成像。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the MRI imaging of glioblastoma multiforme with nanoparticles biomimetic to macrophage cell membrane (Fe3O4 NCs@MM). Methods: The nanoparticles with macrophage cell membrane (Fe3O4 NCs@MM) were prepared and characterized by Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to evaluate the complete coating of extracted macrophage cell membranes. The anti-protein adsorption ability of nanoparticles with macrophage cell membrane was determined by UV-VIS spectroscopy. The T1 relaxation effects of Fe3O4NCs@MM with Fe at different concentrations (0.1-1.6 mM) in the presence or absence of GSH were analyzed by MRI. Cell Counting Kit-8 (CCK-8) was used to determine the cell activity of tumor cells treated with macrophage cell membrane bionic nanoparticles for 24 h. Glioblastoma-bearing mice were injected with macrophage cell membrane biomimetic nanoparticles and scanned by MRI. Results: The hydration kinetic particle size and surface potential of macrophage cell membrane biomimetic nanoparticle (Fe3O4 NCs@MM) were 286.5±7.6 nm and -20.7±3.5 mV respectively, with good monodispersion. Nanoparticles coated with macrophage cell membrane can successfully resist protein adsorption. MRI imaging showed that the prepared macrophage membrane bionic nanoparticles (Fe3O4 NCs@MM) were considered as GSH-responsive MRI contrast agents for T1-weighted MRI imaging. The enhanced signal at the tumor site can be observed at 0.5 h post-injection of Fe3O4 NCs @MM. Conclusion: MRI imaging of glioblastoma multiforme can be achieved by biomimetic nanoparticles of macrophage cell membrane (Fe3O4 NCs@MM). |
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