文章摘要
周文志,张 莉,王文燕,史雅静,张晶晶,郭丽梅,赵跃斌.2型糖尿病足患者肠道菌群特征及与临床生化指标相关性研究[J].,2023,(24):4765-4770
2型糖尿病足患者肠道菌群特征及与临床生化指标相关性研究
A Study on the Distribution Characteristics of Intestinal Flora in Type 2 Diabetic Foot Patients and its Correlation
投稿时间:2023-04-07  修订日期:2023-04-28
DOI:10.13241/j.cnki.pmb.2023.24.033
中文关键词: 2型糖尿病足  肠道菌群  肠道菌群紊乱  16S rRNA测序
英文关键词: Diabetic foot  Intestinal flora  Disturbances of the intestinal flora  16S rRNA gene sequencing
基金项目:山西省应用基础研究项目(201801D121361)
作者单位E-mail
周文志 山西医科大学第九临床医学院 山西 太原 030000 wyzhouwenzhi@163.com 
张 莉 太原市中心医院内分泌科 山西 太原 030000  
王文燕 太原市中心医院内分泌科 山西 太原 030000  
史雅静 太原市中心医院内分泌科 山西 太原 030000  
张晶晶 太原市中心医院内分泌科 山西 太原 030000  
郭丽梅 平遥县中医院 内分泌科 山西 平遥 031100  
赵跃斌 山西医科大学第九临床医学院 山西 太原 030000太原市中心医院内分泌科 山西 太原 030000  
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中文摘要:
      摘要 目的:通过16S rRNA测序技术检测研究对象肠道菌群,探讨2型糖尿病足患者肠道菌群特征及其与主要临床生化指标的相关性,为基于肠道菌群途径治疗糖尿病足提供新的理论基础。方法:选取研究对象分为糖足组(A组,n=32)、糖尿病组(B组,n=32)、对照组(C组,n=31)三组,收集三组研究对象粪便标本及空腹血糖、糖化血红蛋白、炎症指标等主要临床生化指标,采用16S rRNA测序技术检测受试者肠道菌群,分析2型糖尿病足患者肠道菌群特征及其与主要临床化验指标的相关性。结果:1.与糖尿病组和对照组比较,糖足组血糖、血脂水平、炎性指标明显升高(P<0.05)。2.糖足组物种丰富度明显小于其他两组(P<0.05),三组间α多样性和?茁多样性存在差异(P<0.05)。3.糖足组中,弧菌属(尤以脱硫弧菌)、粪球菌属、厌氧棍状菌属等有害标志性物种丰度较其他两组增加,而双歧杆菌属、阿克曼菌属、普氏栖粪杆菌属、乳杆菌属等有益标志性菌种较其他两组减少。4.Spearman相关性分析表明瘤胃球菌属与FPG、HbA1c、WBC、NE%、CRP、 ESR等指标成负相关(P<0.05),志贺菌属与WBC、NE%、CRP、ESR等指标成正相关(P<0.05),脱硫弧菌属与FPG、HbA1c、TG、WBC、NE%、CRP等指标成正相关(P<0.05)。结论:糖足组肠道菌群更加紊乱,而紊乱的肠道菌群特征与糖足组更加紊乱的糖代谢、脂质代谢以及更严重的炎症状态有关。因此,肠道菌群紊乱可能与2型糖尿病足的发生发展密切相关。
英文摘要:
      ABSTRACT Objective: 16S rRNA sequencing technology was used to detect the intestinal flora of the research subjects, explore the intestinal flora characteristics and its correlation with Clinical biochemical index in patients wihe type 2 diabetic foot, and provide a new theoretical basis for the treatment of diabetic foot based on intestinal flora. Methods: The research objects were divided into three groups: diabetic foot group (A, n=32), diabetic group (B, n=32), and control group (C, n=31), and collected fecal samples and clinical biochemical indexes such as fasting blood glucose, HbA1c and inflammatory indexes. 16S rRNA sequencing technology was used to detect the intestinal flora of the objects and analyze the intestinal flora characteristics of patients with type 2 diabetic foot and its correlation with clinical biochemical indexes. Results: 1. Compared with the diabetic group and control group, blood glucose, lipid levels and inflammatory indexes were significantly increased in the diabetic foot group(P<0.05). 2. The species richness of the diabetic foot group was significantly lower than that of the other two groups (P<0.05), and there were differences in alpha diversity and β diversity among the three groups (P<0.05). 3. In the diabetic foot group, the abundance of harmful iconic species such as Vibrio (especially desulfovibrio), Coprococcus and anaerobic Claviculus increased compared with the other two groups, while the beneficial iconic species such as bifidobacterium, Akkermania, Coprobiotic and Lactobacillus decreased compared with the other two groups. 5.Spearman correlation analysis showed that Ruminococcus was negatively correlated with FPG, HbA1c, WBC, NE%, CRP and ESR(P<0.05), while Shigella was positively correlated with WBC, NE%, CRP and ESR(P<0.05). Desulfovibrio was positively correlated with FPG, HbA1c, TG, WBC, NE% and CRP(P<0.05). Conclusion: The intestinal flora in the diabetic foot group was more disordered, and the characteristics of the disordered intestinal flora were associated with more disordered glucose metabolism, lipid metabolism and more severe inflammation in the diabetic foot group. Therefore, intestinal flora disorders may be closely related to the occurrence and development of type 2 diabetic foot.
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