文章摘要
鲍新坤,林爱珍,张晓雨,肖文洁,孙光军.湿润烧伤膏对肛瘘术后造模大鼠创面修复的影响[J].,2023,(24):4642-4646
湿润烧伤膏对肛瘘术后造模大鼠创面修复的影响
Effect of Moist Exposed Burn Ointment on Wound Healing in Rats after Anal Fistula Operation
投稿时间:2023-08-09  修订日期:2023-08-31
DOI:10.13241/j.cnki.pmb.2023.24.007
中文关键词: 湿润烧伤膏  肛瘘  大鼠  创面修复
英文关键词: Moist exposed burn ointment  Anal fistula  Rat  Wound healing
基金项目:湖北省卫生健康委员会中医药科研青年项目(ZY2021Q034)
作者单位E-mail
鲍新坤 湖北省中医院肛肠科 湖北 武汉 430000 13871177938@163.com 
林爱珍 湖北省中医院肛肠科 湖北 武汉 430000  
张晓雨 湖北省中医院肛肠科 湖北 武汉 430000  
肖文洁 湖北省中医院肛肠科 湖北 武汉 430000  
孙光军 湖北省中医院肛肠科 湖北 武汉 430000  
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中文摘要:
      摘要 目的:探究湿润烧伤膏(MEBO)对肛瘘术后造模大鼠创面修复的影响。方法:将45只SPF级SD大鼠随机分为模型组(Model组)、MEBO组、易孚组,每组15只。建立全层皮肤缺损开放感染模型。观察肛瘘创面组织一般情况。分别在给药第3、7、14天测定并计算创面愈合率。苏木精-伊红(HE)染色观察大鼠创面组织病理学变化。酶联免疫吸附实验(ELISA)检测各组大鼠创面肉芽组织中炎症因子指标[肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)]水平。采用碱性磷酸酶(SAP)免疫组化法对创面肉芽组织进行染色,观察表皮生长因子(EGF)、基质金属蛋白酶-2(MMP-2)水平。蛋白质印迹(Western Blot)法检测各组大鼠创面肉芽组织B细胞淋巴瘤/白血病-xL基因(Bcl-xl)、B细胞淋巴瘤/白血病-2基因拮抗剂(Bak)蛋白表达水平,计算Bcl-xl/Bak比值。结果:与Model组相比,MEBO组大鼠创面愈合率(干预7、14 d后)、创面肉芽组织中EGF、MMP-2水平、Bcl-xl、Bak蛋白表达水平均显著升高(P<0.05),创面分泌物和水肿评分、创面肉芽组织生长评分、创面肉芽组织中TNF-α、IL-6水平、Bcl-xl/Bak比值均显著降低(P<0.05)。大鼠创面组织出血减少、细胞间渗出液减少,血管扩张减轻,炎性细胞浸润减轻,可见大量的新生毛细血管和成纤维细胞。结论:MEBO可能通过抑制炎症反应,改善创面微循环和促进组织再生对肛瘘术后大鼠创面修复。
英文摘要:
      ABSTRACT Objective: To explore the effect of moist exposed burn ointment (MEBO) on wound healing in rats after anal fistula operation. Methods: 45 SPF SD rats were randomly divided into model group (Model group), MEBO group and Yi Fu group, with 15 rats in each group. An open infection model of full-thickness skin defect was established. The general situation of anal fistula wound tissue were observed. The wound healing rate was measured and calculated on the 3rd, 7th and 14th day of administration. The pathological changes of wound tissue in rats were observed by hematoxylin-eosin (HE) staining. The levels of inflammatory factors [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)] in granulation tissue of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA). The granulation tissue of the wound were stained by alkaline phosphatase(SAP) immunohistochemical method, and the levels of epidermal growth factor(EGF) and matrix metalloproteinase-2 (MMP-2) were observed. The protein expression levels of B-cell lymphoma/leukemia-xL gene (Bcl-xl) and B-cell lymphoma/leukemia 2 gene antagonist (Bak) in wound granulation tissue of rats in each group were detected by western blot (Western Blot), and the Bcl-xl/Bak ratio was calculated. Results: Compared with Model group, the wound healing rate (after 7 and 14 days of intervention), EGF, MMP-2 levels, Bcl-xl and Bak protein expression levels in wound granulation tissue of rats in MEBO group were significantly increased(P<0.05), the wound secretion and edema score, wound granulation tissue growth score, TNF-α, IL-6 levels in wound granulation tissue, and Bcl-xl/Bak ratio were significantly decreased(P<0.05). The bleeding of wound tissue in rats was reduced, the intercellular exudate was reduced, the vasodilation was reduced, the infiltration of inflammatory cells was reduced, and a large number of new capillaries and fibroblasts were seen. Conclusion: MEBO may improve wound microcirculation and promote tissue regeneration by inhibiting inflammatory response in rats after anal fistula operation.
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