| 唐一萍,冯 俊,彭 涛,李志勇,马 鹏,罗德艳.喉鳞状细胞癌组织miR-1207-5p、miR-186-5p表达水平与PI3K/Akt信号通路、临床病理特征和预后的关系[J].,2023,(22):4241-4246 |
| 喉鳞状细胞癌组织miR-1207-5p、miR-186-5p表达水平与PI3K/Akt信号通路、临床病理特征和预后的关系 |
| Relationship between the Expression Levels of miR-1207-5p and miR-186-5p in Laryngeal Squamous Cell Carcinoma Tissue and PI3K/Akt Signaling Pathway, Clinicopathological Features and Prognosis |
| 投稿时间：2023-05-23 修订日期：2023-06-11 |
| DOI：10.13241/j.cnki.pmb.2023.22.008 |
| 中文关键词: 喉鳞状细胞癌 miR-1207-5p miR-186-5p PI3K/Akt信号通路 临床病理特征 预后 |
| 英文关键词: Laryngeal squamous cell carcinoma MiR-1207-5p MiR-186-5p PI3K/Akt signaling pathway Clinicopathological features Prognosis |
| 基金项目:四川省教育厅基金重点项目（18ZA0203） |
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| 中文摘要: |
| 摘要 目的：探讨喉鳞状细胞癌（LSCC）组织miR-1207-5p、miR-186-5p表达水平与磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/Akt）信号通路、临床病理特征和预后的关系。方法：选取2017年1月至2020年1月南充市中心医院收治的120例LSCC患者，取手术切除的LSCC组织和癌旁组织。检测miR-1207-5p、miR-186-5p、PI3K mRNA、Akt mRNA表达。患者出院后随访3年，统计总生存（OS）和无复发生存（RFS）情况。分析miR-1207-5p、miR-186-5p与PI3K、Akt的相关性以及影响LSCC患者预后的因素。结果：LSCC组织miR-1207-5p、miR-186-5p表达低于癌旁组织（P＜0.05），PI3K mRNA、Akt mRNA表达高于癌旁组织（P＜0.05）。LSCC组织miR-1207-5p、miR-186-5p表达与PI3K mRNA、Akt mRNA表达呈负相关（P＜0.05）。肿瘤直径≥1 cm、低分化、TNM分期Ⅲ期、颈部淋巴结转移LSCC组织中miR-1207-5p、miR-186-5p表达低于肿瘤直径＜1 cm、中高分化、TNM分期Ⅰ～Ⅱ期、无颈部淋巴结转移（P＜0.05）。miR-1207-5p低表达、miR-186-5p低表达LSCC患者3年总生存（OS）率和无复发生存（RFS）率低于miR-1207-5p高表达、miR-186-5p高表达LSCC患者（P＜0.05）。多因素COX回归分析显示TNM III期、颈部淋巴结转移是LSCC患者复发和死亡的危险因素（P＜0.05），高miR-1207-5p、高miR-186-5p是保护因素（P＜0.05）。结论：LSCC组织中miR-1207-5p和miR-186-5p表达均下调，与LSCC恶性病理特征、PI3K/Akt信号通路激活以及低生存率有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression levels of miR-1207-5p and miR-186-5p in laryngeal squamous cell carcinoma (LSCC) tissue and the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway, clinicopathological features and prognosis. Methods: 120 patients with LSCC admitted to Nanchong Central Hospital from January 2017 to January 2020 were selected, and the surgically resected LSCC tissue and paracancer tissue were taken . The expression of miR-1207-5p, miR-186-5p, PI3K mRNA and Akt mRNA were detected. After discharge, the patient was followed up for 3 years to calculate the overall survival (OS) and recurrence free survival (RFS). The correlation of miR-1207-5p and miR-186-5p with PI3K and Akt and the factors affecting the prognosis of LSCC patients were analyzed. Results: The expressions of miR-1207-5p and miR-186-5p in LSCC tissue were lower than those in paracancer tissue(P<0.05), and the expressions of PI3K mRNA and Akt mRNA in LSCC tissue were higher than those in paracancer tissue (P<0.05). The expressions of miR-1207-5p and miR-186-5p in LSCC tissue were negatively correlated with the expressions of PI3K mRNA and Akt mRNA(P<0.05). The expressions of miR-1207-5p and miR-186-5p in LSCC tissue with tumor diameter ≥1 cm, poorly differentiated, TNM stage Ⅲ, and cervical lymph node metastasis were lower than those in LSCC tissue with tumor diameter < 1 cm, medium and highly differentiated, TNM stage Ⅰ to Ⅱ, and no cervical lymph node metastasis (P<0.05). The 3-year overall survival (OS) rate and relapse-free survival (RFS) rate of LSCC patients with low expression of miR-1207-5p and low expression of miR-186-5p were lower than those with high expression of miR-1207-5p and high expression of miR-186-5p LSCC patients(P<0.05). Multivariate COX regression analysis showed that TNM stage III and cervical lymph node metastasis were risk factors for recurrence and death in LSCC patients (P<0.05), and high miR-1207-5p, high miR-186-5p were protective factors (P<0.05). Conclusion: The down-regulated expression of miR-1207-5p and miR-186-5p in LSCC tissue were related to the malignant pathological characteristics of LSCC, activation of PI3K/Akt signaling pathway and low survival rate. |
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