| 刘莉莉,杨 丹,陈 敏,王晶晶,王梓屹.漏芦醇提取物对小鼠乳腺上皮细胞氧化损伤的缓解作用[J].,2023,(22):4212-4217 |
| 漏芦醇提取物对小鼠乳腺上皮细胞氧化损伤的缓解作用 |
| Alleviative Effect of Rhaponticum Uniflorum Ethanol Extract on Oxidative Damage in Mouse Mammary Epithelial Cells |
| 投稿时间：2023-06-06 修订日期：2023-06-30 |
| DOI：10.13241/j.cnki.pmb.2023.22.003 |
| 中文关键词: 漏芦 乳腺上皮细胞 脂多糖 氧化应激 |
| 英文关键词: Rhaponticum uniflorum Mammary epithelial cells Lipopolysaccharide Oxidative stress |
| 基金项目:国家自然科学基金项目（82003930） |
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| 中文摘要: |
| 摘要 目的：探讨漏芦乙醇提取物（RUEE）对脂多糖（LPS）诱导的小鼠乳腺上皮细胞氧化损伤发挥保护作用。方法：采用体外培养的小鼠乳腺上皮细胞系 HC11 为模型，首先利用MTT法筛选RUEE的最佳作用浓度及检测LPS和RUEE对细胞活力的影响，然后用1 μg?mL-1 LPS单独处理，20、40、80 μg?mL-1的RUEE与 1 μg?mL-1 LPS 共处理 HC11细胞，检测氧化应激相关指标、抗氧化相关基因mRNA和蛋白表达的变化。结果：细胞活力实验确定20、40、80 μg?mL-1的RUEE作为后续试验的添加浓度。LPS诱导可显著提高HC11细胞内丙二醛（MDA）含量、一氧化氮（NO）水平、一氧化氮合酶（iNOS）活性（P＜0.05）；明显降低超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）的活性和总抗氧化能力（T-AOC）（P＜0.05）；显著抑制核因子E2相关因子2（Nrf2）、血红素氧合酶1（HO-1）、NAD(P)H醌氧化还原酶1（NQO1）的mRNA及蛋白表达（P＜0.05）。20、40、80 μg?mL-1的RUEE预处理后可明显下调LPS诱导的HC11细胞内MDA含量、NO水平及iNOS活性（P＜0.05）；显著提高SOD、GPx、CAT的活性及T-AOC（P＜0.05）；显著上调Nrf2、HO-1、NQO1 mRNA和蛋白表达（P＜0.05）。结论：RUEE可以有效减轻LPS诱导的乳腺上皮细胞氧化损伤，这可能与RUEE能激活乳腺上皮细胞Nrf2进而促进抗氧化基因的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: The purpose of this article was to investigate whether the ethanol extract of Rhaponticum uniflorum (RUEE) could protect the mouse mammary epithelial cells from oxidative damage induced by lipopolysaccharide (LPS). Methods: In the experiment, Using the mouse mammary epithelial cell line HC11 cultured in vitro as the model, the MTT method was firstly used to screen the optimal concentration of RUEE and detect the effects of LPS and RUEE on cell viability. Then, 1 μg?mL-1 LPS, and 20, 40, 80 μg?mL-1 RUEE + 1 μg?mL-1 LPS respectively were added into the culture medium of HC11 cells. The items related to oxidative stress and the mRNA and proteins expression levels of antioxidant-related genes were detected. Results: Cell viability experiments identified 20, 40, and 80 μg?mL-1 RUEE were used as the additive concentration for subsequent experiments. LPS induction could significantly increased the content of malondialdehyde (MDA), the level of nitric oxide (NO) and the activity of nitric oxide synthase (iNOS) in HC11 cells(P＜0.05); LPS induction could obviously decreased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and the level of total antioxidant capacity (T-AOC) (P＜0.05); LPS induction could evidently down-regulated the mRNA and protein expression of nuclear factor E2 related factor 2(Nrf2), heme oxygenase 1(HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1)(P＜0.05). After pretreatment with 20, 40, 80 μg?mL-1 of RUEE, the MDA level, NO content and iNOS activity in HC11 cells stimulated by LPS were significantly reduced(P＜0.05); The activities of SOD, GPx, CAT and T-AOC were obviously elevated(P＜0.05); the mRNA and protein expression of Nrf2, HO-1 and NQO1 were prominently up-regulated(P＜0.05). Conclusion: It indicates that RUEE can effectively alleviate LPS induced-oxidative damage to mammary epithelial cells, which may be related to RUEE activating Nrf2 protein in mammary epithelial cells and promoting the expression of downstream antioxidant genes. |
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