| 刘亚兵,李 婧,武 莉,赵永炜,许 瑞.丙泊酚对老年大鼠的认知功能障碍及海马神经元凋亡的影响[J].,2023,(20):3826-3830 |
| 丙泊酚对老年大鼠的认知功能障碍及海马神经元凋亡的影响 |
| Effects of Propofol on Cognitive Dysfunction and Hippocampal Neuron Apoptosis in Aged Rats |
| 投稿时间：2023-04-07 修订日期：2023-04-30 |
| DOI：10.13241/j.cnki.pmb.2023.20.005 |
| 中文关键词: 丙泊酚 老年 大鼠 认知功能 海马神经元 凋亡 |
| 英文关键词: Propofol Old age Rat Cognitive function Hippocampal neurons Apoptosis |
| 基金项目:山西省基础研究计划（自由探索类）项目（202203021211225） |
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| 中文摘要: |
| 摘要 目的：探讨丙泊酚对老年大鼠的认知功能障碍及海马神经元凋亡的影响。方法：选择45只老年大鼠随机分为3组，包括对照组、七氟烷组、联合组，七氟烷组吸入1.5%七氟烷，吸入频次为1 h/d；联合组则给予1.5%七氟烷、丙泊酚注射液150 mg/kg，对照组则等量注射氯化钠溶液，3组均进行为期2周的给药时间。进行Morris水迷宫试验，对海马神经元凋亡蛋白，血清白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）水平，PI3K/Akt/ mTOR通路蛋白相对表达予以检测。结果：Morris水迷宫试验2、3 d时潜伏期、游泳距离比Morris水迷宫试验1 d时缩短，七氟烷组Morris水迷宫试验1、2 d时潜伏期比对照组延长，Morris水迷宫试验1、2 d时游泳距离比对照组延长，联合组第1、2、3 d潜伏期比七氟烷组缩短，游泳距离比七氟烷组缩短（P<0.05）；与对照组相比，七氟烷组Bax蛋白、Capase-3蛋白相对表达水平升高，Bcl-2蛋白相对表达水平下降；与七氟烷组相比，联合组Bax蛋白、Capase-3蛋白相对表达水平下降，Bcl-2蛋白相对表达水平上升（P<0.05）；七氟烷组血清IL-6、TNF-α、IL-1β水平比对照组高，联合组血清IL-6、TNF-α、IL-1β水平比七氟烷组低（P<0.05）；七氟烷组p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR通路蛋白相对表达比对照组低，联合组p-PI3K/PI3K、p-Akt/Akt、p-mTOR/mTOR通路蛋白相对表达比七氟烷组高（P<0.05）。结论：丙泊酚可有效缓解七氟烷引起的老年大鼠神经受损状况，其与该药物通过对炎症反应与海马细胞凋亡的抑制，对PI3K/Akt/ Mtor信号通路进行激活有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of propofol on cognitive dysfunction and hippocampal neuron apoptosis in aged rats. Methods: Forty-five aged rats were randomly divided into 3 groups, including control group, sevoflurane group and combination group. The sevoflurane group received 1.5% sevoflurane with an inhalation frequency of 1 h/ day. The combined group was given sevoflurane 1.5% and propofol injection 150 mg/kg, while the control group was given sodium chloride solution in the same amount. All the three groups were given the administration time for 2 weeks. Morris water maze test was performed to detect the levels of hippocampal neuronal apoptosis protein, serum interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and the relative expression of PI3K/Akt/ mTOR pathway protein. Results: The incubation period and swimming distance of the Morris water maze test at 2 and 3 days were shorter than that of the Morris water maze test at 1 day. The incubation period of the sevoflurane group at 1 and 2 days was longer than that of the control group, and the swimming distance of the Morris water maze test at 1 and 2 days was longer than that of the control group. Compared with sevoflurane group, the incubation period and swimming distance in combination group were shorter at the first, second and third days (P<0.05). The relative expression levels of Bax protein and Capase-3 protein in sevoflurane group were higher than those in the control group, the relative expression levels of Bcl-2 protein were lower than those in the control group, and the relative expression levels of Bax protein and Capase-3 protein in the combination group were lower than those in the sevoflurane group. The relative expression level of Bcl-2 protein was higher than that of sevoflurane group (P<0.05). Serum levels of IL-6, TNF-α and IL-1β in sevoflurane group were higher than those in control group, and serum levels of IL-6, TNF-α and IL-1β in combination group were lower than those in sevoflurane group (P<0.05). The protein relative expressions of P-PI3K /PI3K, p-Akt/Akt and P-Mtor /mTOR pathways in sevoflurane group were lower than those in control group, and the protein relative expressions of P-PI3K /PI3K, p-Akt/Akt and P-Mtor /mTOR pathways in combination group were higher than those in sevoflurane group (P<0.05). Conclusion: Propofol can effectively relieve sevoflurane induced nerve damage in aged rats, which is related to the activation of PI3K/Akt/ Mtor signaling pathway by inhibiting inflammatory response and hippocampal cell apoptosis. |
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