| 王 燕,高 婉,王 聪,李亚萍,李冰琳,张淑会.宫颈癌组织miR-200b-5p、miR-424-5p表达与临床病理特征、PI3K/AKT/mTOR信号通路和预后的关系研究[J].,2023,(19):3714-3718 |
| 宫颈癌组织miR-200b-5p、miR-424-5p表达与临床病理特征、PI3K/AKT/mTOR信号通路和预后的关系研究 |
| Relationship Study between miR-200b-5p and miR-424-5p Expression in Cervical Cancer Tissues and Clinicopathological Features, PI3K/AKT/mTOR Signaling Pathway and Prognosis |
| 投稿时间：2023-03-07 修订日期：2023-03-28 |
| DOI：10.13241/j.cnki.pmb.2023.19.023 |
| 中文关键词: 宫颈癌 miR-200b-5p miR-424-5p 病理特征 PI3K/AKT/mTOR信号通路 预后 |
| 英文关键词: Cervical cancer miR-200b-5p miR-424-5p Clinicopathological features PI3K/AKT/mTOR signaling pathway Prognosis |
| 基金项目:陕西省自然科学基金项目（2121JM-563） |
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| 中文摘要: |
| 摘要 目的：探讨宫颈癌组织微小核糖核酸（miRNA）-200b-5p、miR-424-5p表达与临床病理特征、磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白（PI3K/AKT/mTOR）信号通路和预后的关系。方法：选取2016年7月～2019年6月西安市中心医院收治的123例宫颈癌患者，采用定量聚合酶链式反应（qPCR）检测癌组织与癌旁组织中miR-200b-5p、miR-424-5p、PI3K信使RNA（mRNA）、AKT mRNA、mTOR mRNA表达。分析miR-200b-5p、miR-424-5p表达与PI3K mRNA、AKT mRNA、mTOR mRNA表达的相关性及与临床病理特征的关系。采用K-M法绘制不同miR-200b-5p、miR-424-5p表达宫颈癌患者生存曲线。结果：与癌旁组织比较，宫颈癌组织中miR-200b-5p、miR-424-5p表达降低，PI3K mRNA、AKT mRNA、mTOR mRNA表达升高（P＜0.05）。Pearson相关性分析显示，宫颈癌组织中miR-200b-5p、miR-424-5p表达与PI3K mRNA、AKT mRNA、mTOR mRNA表达均呈负相关，PI3K mRNA、AKT mRNA、mTOR mRNA表达呈两两相关（P＜0.05）。miR-200b-5p、miR-424-5p表达与宫颈癌分化程度、国际妇产科联盟（FIGO）分期和淋巴结转移有关（P＜0.05）。随访3年，123例宫颈癌患者累积生存率为62.60%（77/123）。K-M生存曲线分析显示，miR-200b-5p、miR-424-5p高表达组累积生存率分别高于miR-200b-5p、miR-424-5p低表达组（P＜0.05）。结论：宫颈癌组织中miR-200b-5p、miR-424-5p低表达，与分化程度、FIGO分期、淋巴结转移、PI3K/AKT/mTOR信号通路和预后有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between microribonucleic acid (miRNA)-200b-5p and miR-424-5p expression in cervical cancer tissues and clinicopathological features, phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway and prognosis. Methods: 123 patients with cervical cancer who were admitted to Xi'an Central Hospital from July 2016 to June 2019 were selected. Quantitative polymerase chain reaction (qPCR) was used to detect the expressions of miR-200b-5p, miR-424-5p, PI3K messenger RNA (mRNA), AKT mRNA and mTOR mRNA in cancer tissues and paracancer tissues. The correlation between the expressions of miR-200b-5p and miR-424-5p and the expressions of PI3K mRNA, AKT mRNA and mTOR mRNA and their relationship with clinicopathological features were analyzed. Survival curves of patients with cervical cancer with different expressions of miR-200b-5p and miR-424-5p were plotted by K-M method. Results: Compared with paracancer tissues, the expressions of miR-200b-5p and miR-424-5p in cervical cancer tissues were decreased, while the expressions of PI3K mRNA, AKT mRNA and mTOR mRNA were increased (P＜0.05). Pearson correlation analysis showed that the expressions of miR-200b-5p and miR-424-5p in cervical cancer tissues were negatively correlated with the expressions of PI3K mRNA, AKT mRNA and mTOR mRNA, the expressions of PI3K mRNA, AKT mRNA and mTOR mRNA were pairwise correlated (P＜0.05). The expressions of miR-200b-5p and miR-424-5p were correlated with the degree of cervical cancer differentiation, International Federation of Gynecology and Obstetrics (FIGO) staging and lymph node metastasis (P＜0.05). Following up for 3 years, the cumulative survival rate of 123 patients with cervical cancer was 62.60% (77/123). K-M survival curve analysis showed that the cumulative survival rate of miR-200b-5p and miR-424-5p high-expression groups were higher than that of miR-200b-5p and miR-424-5p low-expression groups, respectively (P＜0.05). Conclusion: The low expression of miR-200b-5p and miR-424-5p in cervical cancer tissues is related to differentiation degree, FIGO stage, lymph node metastasis, PI3K/AKT/mTOR signaling pathway and prognosis. |
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