| 张泽宇,谢 潭,王飞通,魏 鑫,刘 斌.肝细胞癌组织miR-124-3p、miR-212-5p表达与PI3K/Akt信号通路和预后的关系研究[J].,2023,(19):3625-3629 |
| 肝细胞癌组织miR-124-3p、miR-212-5p表达与PI3K/Akt信号通路和预后的关系研究 |
| Relationship between the Expression of miR-124-3p and miR-212-5p in Hepatocellular Carcinoma Tissue and the PI3K/Akt Signaling Pathway and Prognosis |
| 投稿时间：2023-03-28 修订日期：2023-04-23 |
| DOI：10.13241/j.cnki.pmb.2023.19.005 |
| 中文关键词: 肝细胞癌 PI3K/Akt信号通路 miR-124-3p miR-212-5p 预后 |
| 英文关键词: Hepatocellular carcinoma PI3K/Akt signaling pathway miR-124-3p miR-212-5p Prognosis |
| 基金项目:江苏省高等学校自然科学研究面上项目（17KJD320004） |
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| 中文摘要: |
| 摘要 目的：探讨肝细胞癌组织中微小核糖核酸（miR）-124-3p、miR-212-5p表达与磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（Akt）信号通路和预后的关系。方法：选择2017年9月至2019年9月徐州医科大学附属医院肝胆外科收治的93例肝细胞癌患者，采用实时荧光定量聚合酶链反应（qRT-PCR）检测肝细胞癌组织中miR-124-3p、miR-212-5p、PI3K 信使RNA（mRNA）、Akt mRNA的表达。Pearson相关性分析miR-124-3p、miR-212-5p表达与PI3K/Akt信号通路相关mRNA表达的相关性。绘制Kaplan-Meier生存曲线，Log-Rank检验不同miR-124-3p、miR-212-5p表达肝细胞癌患者3年总生存率（OS）的差异。结果：肝细胞癌组织中miR-124-3p表达低于癌旁组织（P＜0.05），miR-212-5p、PI3K mRNA、Akt mRNA表达高于癌旁组织（P＜0.05）。肝细胞癌组织中miR-124-3p表达与PI3K mRNA、Akt mRNA表达呈负相关（P＜0.05），miR-212-5p表达与PI3K mRNA、Akt mRNA表达呈正相关（P＜0.05）。Ⅱa期、低分化患者肝细胞癌组织中miR-124-3p表达低于Ⅰa-Ⅰb期、中高分化患者（P＜0.05），miR-212-5p表达高于Ⅰa-Ⅰb期、中高分化患者（P＜0.05）。随访期间死亡37例， miR-124-3p低表达组3年OS为42.55%，低于miR-124-3p高表达组的78.26%（P＜0.05），miR-212-5p高表达组3年OS为50.00%，低于miR-212-5p低表达组的71.11%（P＜0.05）。结论：肝细胞癌组织中miR-124-3p表达下调，miR-212-5p表达上调，且与肝细胞癌PI3K/Akt信号通路激活，PI3K mRNA和Akt mRNA高表达，低分化，CNLC分期Ⅱa期以及低OS有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression of microribonucleic acid(miR)-124-3p and miR-212-5p in hepatocellular carcinoma tissues and the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway and prognosis. Methods: 93 patients with hepatocellular carcinoma who were admitted to the Department of Hepatobiliary Surgery of Xuzhou Medical University Affiliated Hospital were selected from September 2017 to September 2019, the expression of miR-124-3p, miR-212-5p, PI3K messenger RNA (mRNA), Akt mRNA in hepatocellular carcinoma tissue was detected by real-time quantitative polymerase chain reaction (qRT-PCR). The correlation between miR-124-3p, miR-212-5p expression and PI3K/Akt signaling pathway related mRNA expression were analyzed by Pearson correlation. Drawed Kaplan-Meier survival curve and Log-Rank test for differences in 3-year overall survival rate (OS) of hepatocellular carcinoma patients with different miR-124-3p and miR-212-5p expression levels. Results: The expression of miR-124-3p in hepatocellular carcinoma tissue was lower than that in adjacent tissue (P<0.05), while the expression of miR-212-5p, PI3K mRNA, and Akt mRNA were higher than those in adjacent tissue(P<0.05). The expression of miR-124-3p in hepatocellular carcinoma tissue was negatively correlated with the expression of PI3K mRNA and Akt mRNA(P<0.05), while the expression of miR-212-5p was positively correlated with the expression of PI3K mRNA and Akt mRNA(P<0.05). The expression of miR-124-3p in hepatocellular carcinoma tissues of stage II a and poorly differentiated patients were lower than those of stage Ⅰa-Ⅰb and moderately well differentiated patients(P<0.05), while the expression of miR-212-5p were higher than those of stage Ⅰa-Ⅰb and moderately well differentiated patients(P<0.05). During the follow-up period, 37 deaths occurred. The 3-year OS in the low expression group of miR-124-3p was 42.55%, lower than 78.26% in the high expression group of miR-124-3p(P<0.05). The 3-year OS in the high expression group of miR-212-5p was 50.00% , lower than 71.11% in the low expression group of miR-212-5p(P<0.05). Conclusion: The expression of miR-124-3p is downregulated and miR-212-5p is upregulated in hepatocellular carcinoma tissue, which is related to the activation of the PI3K/Akt signaling pathway, high expression of PI3K mRNA and Akt mRNA, low differentiation, CNLC stage II a, and low OS. |
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