| 李 浩,艾日法特·艾尼瓦尔,贾金文,艾尼·艾海提,李 娜.苍耳子对慢性鼻-鼻窦炎大鼠炎症反应、NLRP3炎症小体及JAK2STAT3信号通路的影响[J].,2023,(15):2822-2826 |
| 苍耳子对慢性鼻-鼻窦炎大鼠炎症反应、NLRP3炎症小体及JAK2STAT3信号通路的影响 |
| Effects of Xanthium Xanthium on Inflammatory Response, NLRP3 Inflammatory Bodies and JAK2STAT3 Signaling Pathway in Rats with Chronic Nasosinusitis |
| 投稿时间：2023-03-10 修订日期：2023-03-31 |
| DOI：10.13241/j.cnki.pmb.2023.15.004 |
| 中文关键词: 苍耳子 慢性鼻-鼻窦炎 炎症反应 NLRP3炎症小体 JAK2STAT3信号通路 |
| 英文关键词: Cocklebur seed Chronic rhinosinusitis Inflammatory response NLRP3 inflammatories JAK2STAT3 signal path |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2022D01C326） |
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| 中文摘要: |
| 摘要 目的：探讨苍耳子对慢性鼻-鼻窦炎大鼠炎症反应、NLRP3炎症小体及JAK2STAT3信号通路的影响。方法：选择清洁级SD大鼠40只作为研究对象。将40只大鼠随机分为5组。采用鼻腔内注射金黄色葡萄球菌悬浊液的方法构建慢性鼻-鼻窦炎大鼠模型。空白对照组（8只）、模型组（8只）、低剂量组（7.5 mg/kg，8只）、中剂量组（15 mg/kg，8只）和高剂量组（30 mg/kg，8只）。采用埋藏食物小球实验进行嗅觉功能检查，采用qRC-PCR检测JAK2/STAT3信号通路和NLRP3、ACS、caspase-1的mRNA表达水平并采用ELISA法检查大鼠血液样本中肿瘤坏死因子-α（TNF-α）和IL-1β、IL-18的表达水平。结果：建模前，各组大鼠找到食物小球的时间比较（P＞0.05）；治疗后7 d和治疗完成时，与对照组相比，模型组、低剂量组、中剂量组和高剂量组找到食物小球的时间明显更长（P＜0.05），高剂量组找到食物小球的时间明显短于模型组、低剂量组、中剂量组（P＜0.05）。与对照组相比，模型组、低剂量组、中剂量组和高剂量组JAK2 mRNA、STAT3 mRNA相对表达水平明显更高（P＜0.05），高剂量组JAK2 mRNA、STAT3 mRNA相对表达水平明显低于模型组、低剂量组、中剂量组（P＜0.05）。与对照组相比，模型组、低剂量组、中剂量组和高剂量组TNF-α、IL-1β、IL-18表达水平明显更高（P＜0.05），高剂量组TNF-α、IL-1β、IL-18表达水平明显低于模型组、低剂量组、中剂量组（P＜0.05）。与对照组相比，模型组、低剂量组、中剂量组和高剂量组NLRP3 mRNA、ACS mRNA、caspase-1 mRNA相对表达水平明显更高（P＜0.05），高剂量组NLRP3 mRNA、ACS mRNA、caspase-1 mRNA相对表达水平明显低于模型组、低剂量组、中剂量组（P＜0.05）。结论：CRS大鼠采用苍耳子挥发油进行治疗可通过调控JAK2/STAT3信号通路的表达和NLRP3炎症小体的表达，从而抑制下游炎症因子的过度分泌，最终为缓解CRS病情和改善嗅觉功能做出贡献。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Xanthium Xanthium on inflammatory response, NLRP3 inflamma and JAK2STAT3 signaling pathway in rats with chronic nasosinusitis. Methods: Forty clean SD rats were selected as subjects. Forty rats were randomly divided into 5 groups. The rat model of chronic rhinosinusitis was established by injecting Staphylococcus aureus suspension into the nasal cavity. Blank control group (8 PCS), model group (8 PCS), low-dose group (7.5 mg/kg, 8 PCS), medium-dose group (15 mg/kg, 8 PCS) and high-dose group (30 mg/kg, 8 PCS). To examine olfactory function using the buried food pellets test, JAK2/STAT3 signaling pathway and mRNA expression levels of NLRP3, ACS and caspase-1 were detected by qRC-PCR, and tumor necrosis factor-α (TNF-α), IL-1β and IL-18 in blood samples were detected by ELISA. Results: Before modeling, the time of finding food pellets in each group was compared (P>0.05). Compared with the control group, the time of finding food pellets in model group, low-dose group, medium-dose group and high-dose group was significantly longer on day 7 after treatment and at the completion of treatment (P<0.05), and the time of finding food pellets in high-dose group was significantly shorter than that in model group, low-dose group and medium-dose group (P<0.05). Compared with the control group, the relative expression levels of JAK2 mRNA and STAT3 mRNA in model group, low-dose group, medium-dose group and high-dose group were significantly higher (P<0.05). The relative expression levels of JAK2 mRNA and STAT3 mRNA in high-dose group were significantly lower than those in model group, low-dose group and medium-dose group (P<0.05). Compared with the control group, the expression levels of TNF-α, IL-1β and IL-18 in model group, low-dose group, medium-dose group and high-dose group were significantly higher (P<0.05), while the expression levels of TNF-α, IL-1β and IL-18 in high-dose group were significantly lower than those in model group, low-dose group and medium-dose group (P<0.05). Compared with the control group, the relative expression levels of NLRP3 mRNA, ACS mRNA and caspase-1 mRNA in model group, low-dose group, medium-dose group and high-dose group were significantly higher (P<0.05). The relative expression levels of NLRP3 mRNA, ACS mRNA and caspase-1 mRNA in high-dose group were significantly lower than those in model group, low-dose group and medium-dose group (P<0.05). Conclusion: The treatment of CRS rats with volatile oil of Xanthium Xanthium can inhibit the oversecretion of downstream inflammatory factors by regulating the expression of JAK2/STAT3 signaling pathway and the expression of NLRP3 inflammatory bodies, and ultimately contribute to the remission of CRS disease and the improvement of olfactory function. |
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