| 王 松,武亚光,刘洪峰,冯 玘,范秀英.HPLC-CAD法测定两性霉素B脂质体中HSPC、DSPG含量分析[J].,2023,(14):2728-2731 |
| HPLC-CAD法测定两性霉素B脂质体中HSPC、DSPG含量分析 |
| Determination of HSPC and DSPG in Amphotericin B Liposomes by HPLC-CAD |
| 投稿时间：2023-02-28 修订日期：2023-03-23 |
| DOI：10.13241/j.cnki.pmb.2023.14.024 |
| 中文关键词: HPLC-CAD 两性霉素B脂质体 HSPC DSPG 含量 |
| 英文关键词: HPLC-CAD Amphotericin B liposome HSPC DSPG Content |
| 基金项目:安徽省教育厅2019年省级质量工程重点项目（2019xqsxzx98） |
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| 中文摘要: |
| 摘要 目的：建立高效液相色谱（HPLC）-电喷雾检测器（CAD）法测定两性霉素B脂质体（L-AMB）中氢化大豆磷脂酰胆碱（HSPC）和磷脂二硬酯酰磷脂酰甘油（DSPG）含量的方法。方法：采用Venusil XBP C 8（4.6 mm×250 mm，5 μm）色谱柱，柱温：30℃；以甲醇-水作为流动相；喷雾压力：56.4 psi，喷雾温度：55℃；梯度洗脱，流速1.0 mL?min-1。结果：L-AMB各磷脂在 5~60 mg?mL-1内与峰面积呈良好的线性关系。HSPC平均回收率为100.06%，平均RSD为0.42；DSPG平均回收率100.03%，平均RSD为0.55。按HPLC-CAD 色谱条件测定L-AMB中HSPC、 DSPG含量分别为1497.66 mg?L-1、4710.93 mg?L-1，且无溶血磷脂检出。采用HPLC-ELSD 法验证，HSPC、 DSPG含量分别为1495.28 mg?L-1、4712.71 mg?L-1， 两种方法测定HSPC、 DSPG含量基本一致。结论：HPLC-CAD 测定L-AMB中HSPC、DSPG含量操作简单、准确，可准确测定L-AMB 中脂质体的质量。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a method for the determination of hydrogenated soybean phosphatidylcholine (HSPC) and phosphatidylglycerol (DSPG) in amphotericin B liposomes (L-AMB) by HPLC and electrospray detector (CAD). Methods: Venusil XBP C 8 (4.6 mm×250 mm, 5 μm) column was used. The column temperature was 30℃. Using methanol-water as mobile phase; Spray pressure: 56.4 psi, spray temperature: 55℃; Gradient elution at flow rate of 1.0 mL?min-1. Results: L-AMB phospholipids had a good linear relationship with peak area in 5~60 mg?mL-1. The average recovery of HSPC was 100.06%, and the average RSDS were 0.42. The average recovery of DSPG was 100.03%, and the average RSDS were 0.55. HSPC and DSPG contents in L-AMB were 1497.66 mg?L-1 and 4710.93 mg?L-1, respectively, and no lysophospholipids were detected by HPLC-CAD. HSPC and DSPG contents were 1495.28 mg?L-1 and 4712.71 mg?L-1, respectively. HSPC and DSPG contents by HPLC-ELSD method were consistent. Conclusion: Determination of HSPC and DSPG in L-AMB by HPLC-CAD is simple and accurate, and can accurately determine the quality of liposome in L-AMB. |
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