| 高芳芳,张 俊,仲莉梅,杨晓娜,张 君.联合检测EB-IgG、IgM，EB-DNA在鼻咽癌中的诊断价值分析[J].,2023,(9):1712-1715 |
| 联合检测EB-IgG、IgM，EB-DNA在鼻咽癌中的诊断价值分析 |
| Diagnostic Value of EB-IgG, IgM and EB-DNA in Nasopharyngeal Carcinoma |
| 投稿时间：2022-10-07 修订日期：2022-10-30 |
| DOI：10.13241/j.cnki.pmb.2023.09.022 |
| 中文关键词: 鼻咽癌 EB病毒 抗体 DNA 诊断 |
| 英文关键词: Nasopharyngeal carcinoma EB virus Antibody DNA diagnosis |
| 基金项目:陕西省重点计划研发项目（2022SF-463） |
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| 中文摘要: |
| 摘要 目的：分析联合检测EB-IgG、IgM，EB-DNA在鼻咽癌中的诊断价值。方法：选择我院自2019年1月至2022年1月接诊的100例鼻咽癌患者作为观察组，另选同期的100例健康体检者作为对照组。使用化学发光免疫夹心法检测血清EB-IgG、IgM，实时荧光定量聚合酶链反应检测血浆EB-DNA表达水平；比较两组EB-IgG、IgM和EB-DNA的阳性率，分析不同临床分期的鼻咽癌患者EB-IgG、IgM和EB-DNA的阳性率及EB-DNA表达水平，使用AUC评价EB-IgG、IgM联合EB-DNA对鼻咽癌的诊断效能，计算单独和联合应用上述指标诊断鼻咽癌的敏感度和特异度。结果：观察组EB-IgG、IgM和EB-DNA的阳性率均明显高于对照组，差异均有统计学意义（P＜0.05）；EB-IgG、IgM和EB-DNA的阳性率及EB-DNA表达水平在不同临床分期的鼻咽癌患者中差异均有统计学意义（P＜0.05），均随着临床分期升高而升高；经ROC曲线分析，EB-IgG、IgM联合EB-DNA诊断鼻咽癌的AUC为0.930，大于单一指标EB-IgG的0.755、IgM的0.740和EB-DNA的0.750，差异均有统计学意义（P＜0.05）；EB-IgG、IgM联合EB-DNA诊断鼻咽癌的敏感度为97.00 %，特异度为96.00 %，准确度为96.50 %。结论：EB-IgG、IgM和EB-DNA均对鼻咽癌具有一定的临床诊断价值，联合检测有助于提高鼻咽癌的诊断效能，值得予以重视应用。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the diagnostic value of EB-IgG, IgM and EB-DNA in nasopharyngeal carcinoma. Methods: A total of 100 nasopharyngeal carcinoma patients admitted to our hospital from January 2019 to January 2022 were selected as the observation group, and another 100 healthy subjects were selected as the control group. Serum EB-IgG and IgM were detected using chemiluminescence immunosandwich assay, and plasma EB-DNA expression levels were detected by real-time fluorescence quantitative polymerase chain reaction; the positive rates of EB-IgG, IgM and EB-DNA in the two groups were compared, and the positive rates of EB-IgG, IgM and EB-DNA and EB-DNA expression levels in nasopharyngeal carcinoma patients with different clinical stages were analyzed using AUC was used to evaluate the diagnostic efficacy of EB-IgG and IgM combined with EB-DNA for nasopharyngeal carcinoma, and to calculate the sensitivity and specificity of the above indicators applied alone and in combination for the diagnosis of nasopharyngeal carcinoma. Results: The positive rates of EB-IgG, IgM and EB-DNA in observation group were significantly higher than those in control group, and the differences were statistically significant (P<0.05). There were significant differences in the positive rates of EB-IgG, IgM and EB-DNA and EB-DNA expression levels in patients with nasopharyngeal carcinoma of different clinical stages (P<0.05), which increased with the increase of clinical stage. ROC curve analysis showed that the AUC of EB-IgG, IgM combined with EB-DNA in the diagnosis of nasopharyngeal carcinoma was 0.930, which was higher than 0.755 of EB-IgG, 0.740 of IgM and 0.750 of EB-DNA, and the differences were statistically significant(P<0.05). The sensitivity, specificity and accuracy of EB-IgG and IgM combined with EB-DNA in the diagnosis of nasopharyngeal carcinoma were 97.00 %, 96.00 % and 96.50 %. Conclusion: EB-IgG, IgM and EB-DNA have certain clinical diagnostic value for nasopharyngeal carcinoma, the combined detection of EB- IgG, IgM and EB-DNA is helpful to improve the diagnostic efficiency of nasopharyngeal carcinoma, which is worthy of attention. |
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