| 张丽辉,李毓新,惠 晶,吴延佳,王晓芳.鼠神经生长因子联合神经节苷脂钠注射液对癫痫大鼠认知功能、血清NSE及海马神经元的影响及机制分析[J].,2023,(8):1442-1446 |
| 鼠神经生长因子联合神经节苷脂钠注射液对癫痫大鼠认知功能、血清NSE及海马神经元的影响及机制分析 |
| Effect of Rat Nerve Growth Factor Combined with Ganglioside Sodium Injection on Cognitive Function, Serum NSE and Hippocampal Neurons in Epileptic Rats and Its Mechanism Analysis |
| 投稿时间：2022-09-14 修订日期：2022-10-10 |
| DOI：10.13241/j.cnki.pmb.2023.08.008 |
| 中文关键词: 鼠神经生长因子 神经节苷脂钠注射液 癫痫 大鼠 |
| 英文关键词: Rat nerve growth factor Ganglioside sodium injection Epilepsy Rat |
| 基金项目:陕西省卫生健康科研项目（2018C0326） |
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| 中文摘要: |
| 摘要 目的：探讨鼠神经生长因子联合神经节苷脂钠注射液对癫痫大鼠认知功能、血清NSE及海马神经元的影响。方法：选择SD雄性大鼠50只，随机分为5组，包括空白组、模型组、A组、B组、C组。空白组大鼠腹腔注射等容量的生理盐水，其余组使用腹腔注射氯化锂-毛果芸香碱的方法建立癫痫大鼠模型。空白组、模型组大鼠用微量注射器注射20 μL无菌生理盐水，A组同时间点给大鼠腹腔注射2000 AU/kg鼠神经生长因子；B组同时间点给大鼠腹腔注射20 μL神经节苷脂钠；C组给大鼠腹腔注射2000 AU/kg鼠神经生长因子联合20 ?滋L神经节苷脂钠。进行Morris水迷宫试验、空间探索试验，检测血清NSE水平与海马神经组织的神经元，检测血清NSE水平、海马神经组织的神经元存活率及核因子kB p56、TLR-4、MyD88蛋白水平。结果：模型组第1、2、3、4、5 d时的游泳总距离、逃避潜伏期较其他四组高，空白组较A组、B组、C组高，B组、A组较C组高（P<0.05），B组、A组对比无差异（P>0.05）；模型组的平台穿越次数、探索有效时间比率较空白组、A组、B组、C组低，空白组较A组、B组、C组低，B组、A组较C组低（P<0.05），B组、A组对比无差异（P>0.05）；模型组的血清NSE水平、核因子kB p56、TLR-4、MyD88蛋白水平较空白组、A组、B组、C组低，海马组织中神经元存活率较高，空白组较A组、B组、C组低，海马组织中神经元存活率较高，B组、A组较C组低，海马组织中神经元存活率较高（P<0.05），B组、A组的NSE水平、海马组织中神经元存活率、核因子kB p56、TLR-4、MyD88蛋白水平对比无差异（P>0.05）。结论：鼠神经生长因子联合神经节苷脂钠注射液可改善癫痫大鼠的认知功能，减轻海马组织炎症反应，可能与其可降低血清NSE及海马组织核因子kB p56、TLR-4、MyD88水平有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of rat nerve growth factor combined with ganglioside sodium injection on cognitive function, serum NSE and hippocampal neurons in epileptic rats. Methods: 50 male Sprague-Dawley rats were randomly divided into 5 groups, including blank group, model group, A group, B group, C group. The rats in the blank group were intraperitoneally injected with the same volume of normal saline, and the rats in the other groups were intraperitoneally injected with lithium chloride and pilocarpine to establish the epilepsy rat model. Rats in blank group and model group were injected with 20 μL sterile normal saline with microsyringes. Rats in A group were intraperitoneally injected with 2000 AU/kg rat nerve growth factor at the same time point. Rats in B group were intraperitoneally injected with 20 μL ganglioside sodium at the same time point. C group was intraperitoneally injected with 2000 AU/kg rat nerve growth factor combined with 20 μL ganglioside sodium. Morris water maze test and space exploration test were conducted to detect serum NSE level and hippocampal neurons, and to detect serum NSE level, neuron survival rate and nuclear factor kB P56, TLR-4 and MyD88 protein levels in hippocampal nerve tissue. Results: The total swimming distance at 1,2,3,4, and 5 d were higher than that of the other four groups, higher in blank than A, B and C, B and A than C (P<0.05), and no difference between groups B and A(P>0.05); The number of platform crossing and exploration effective time ratio were lower than group blank, A, B and C, lower than group A, B and C, B and C(P<0.05), and no difference between group B and A (P>0.05); Serum NSE levels, nuclear factors kB p56, TLR-4 and MyD88 protein levels were lower than blank, A, B, C, higher neuronal survival, A, B, C, lower neuronal survival, C, higher (P<0.05), NSE, neuronal survival, and nuclear factors kB p56, TLR-4 and MyD88 protein levels (P>0.05). Conclusion: Rat nerve growth factor combined with ganglioside sodium injection can improve cognitive function and reduce hippocampal inflammatory response in epileptic rats, which may be related to the reduction of serum NSE and hippocampal nuclear factor kB P56, TLR-4, MyD88 levels. |
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