| 贺艳丽,王运萍,綦春蕾,赵淑华,李玲霞,周福兴,张 潍.ω-3脂肪酸对人滋养层细胞侵袭和血管生成的影响[J].,2023,(6):1010-1016 |
| ω-3脂肪酸对人滋养层细胞侵袭和血管生成的影响 |
| Effects of Omega-3 Fatty Acids on the Invasion and Angiogenesis of Human Trophoblast Cells |
| 投稿时间：2022-08-18 修订日期：2022-09-15 |
| DOI：10.13241/j.cnki.pmb.2023.06.003 |
| 中文关键词: ω-3脂肪酸 滋养层细胞 侵袭 血管生成 三结构域蛋白22 信号转导和转录激活因子1 |
| 英文关键词: ω-3 fatty acids Trophoblast cells Invasion Angiogenesis Tripartite motif-containing 22 Signal transducer and activator of transcription 1 |
| 基金项目:国家自然科学基金青年基金项目（82002735） |
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| 中文摘要: |
| 摘要 目的：探讨ω-3脂肪酸对人滋养层细胞（HTR-8/SVneo）侵袭和血管生成的影响。方法：本实验设置了不同浓度二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）处理组，依次为0、1、50和100 μmol/L EPA组；0、1、50和100 μmol/L DHA组。各组HTR-8/SVneo细胞分别用相应浓度的EPA和DHA培养48 h。然后通过CCK-8法检测细胞增殖，Matrigel Transwell实验检测细胞侵袭。使用EPA和DHA处理的HTR-8/SVneo细胞的上清液培养人脐静脉内皮细胞（HUVEC）6 h，然后检测HUVEC的小管形成能力。通过qRT-PCR和Western blot检测HTR-8/SVneo细胞中三结构域蛋白22（TRIM22）、信号转导和转录激活因子1（STAT1）、p-STAT1（Tyr701）、基质金属蛋白酶2（MMP2）、MMP9和VEGF的表达。结果：与0 μmol/L EPA组或0 μmol/L DHA组相比，50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的OD450nm 、侵袭细胞数量、MMP2和MMP9的蛋白相对表达量均升高（P<0.05）。与0 μmol/L EPA组或0 μmol/L DHA组相比，50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的相对小管长度和VEGF蛋白相对表达量均升高（P<0.05）。与0 μmol/L EPA组或0 μmol/L DHA组相比，50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的TRIM22 mRNA和蛋白相对表达量均升高，而STAT1 mRNA相对表达量和p-STAT1 (Tyr701)蛋白相对表达量均降低（P<0.05）。结论：ω-3脂肪酸处理可促进滋养层细胞的侵袭性和血管生成，其机制可能与TRIM22的上调和STAT1活性的抑制有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of omega-3 fatty acids on the invasion and angiogenesis of human trophoblast cells (HTR-8/SVneo). Methods: Different concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) groups were set up: 0 μmol/L EPA group, 1 μmol/L EPA group, 50 μmol/L EPA group and 100 μmol/L EPA group; 0 μmol/L DHA group, 1 μmol/L DHA group, 50 μmol/L DHA group and 100 μmol/L DHA group. The HTR-8/SVneo cells in each group were cultured with corresponding concentrations of EPA and DHA for 48 h. Then the cell proliferation was detected by the CCK-8 method, and the cell invasion was detected by the Matrigel Transwell test. Human umbilical vein endothelial cells (HUVEC) were cultured for 6 h with the supernatant of HTR-8/SVneo cells treated with EPA and DHA, and then the tubule forming ability of HUVEC was detected. The expression level of tripartite motif-containing 22 (TRIM22), signal transducer and activator of transcription 1 (STAT1), p-STAT1 (Tyr701), matrix metalloproteinase 2 (MMP2), MMP9, MMP9 and VEGF in HTR-8/SVneo cells were detected by qRT-PCR and Western blot. Results: Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, OD450 nm , number of invaded cells, and relative protein expression level of MMP2 and MMP9 in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased (P<0.05). Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, the relative tubule length and the relative expression of VEGF protein in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased (P<0.05). Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, the relative expression levels of TRIM22 mRNA and protein in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased, while the relative expression of STAT1 mRNA and p-STAT1 (Tyr701) protein were decreased (P<0.05). Conclusion: Omega-3 fatty acid treatment can promote the invasiveness and angiogenesis of trophoblast cells. The mechanism may be related to the up-regulation of TRIM22 and the inhibition of STAT1 activity. |
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