| 易 华,袁宏伟,孙勤暖,马秀梅,杜 华.肝细胞肝癌组织GPSM2、GFPT2、SNORA51 mRNA表达与临床病理特征的关系及对预后的影响研究[J].,2023,(4):733-738 |
| 肝细胞肝癌组织GPSM2、GFPT2、SNORA51 mRNA表达与临床病理特征的关系及对预后的影响研究 |
| Relationship between the Expression of GPSM2, GFPT2, SNORA51 mRNA and Clinicopathological Features in Hepatocellular Carcinoma Tissues and Their Impact Study on Prognosis |
| 投稿时间：2022-06-28 修订日期：2022-07-23 |
| DOI：10.13241/j.cnki.pmb.2023.04.026 |
| 中文关键词: 肝细胞肝癌 GPSM2 GFPT2 SNORA51 临床病理特征 预后 |
| 英文关键词: Hepatocellular carcinoma GPSM2 GFPT2 SNORA51 Clinicopathological features Prognosis |
| 基金项目:内蒙古自治区科技计划项目（2021GG0170） |
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| 中文摘要: |
| 摘要 目的：探讨肝细胞肝癌（HCC）组织G蛋白信号调节蛋白2（GPSM2）、谷氨酰胺果糖-6-磷酸转氨酶2（GFPT2）、核仁小RNA 51（SNORA51） mRNA表达与临床病理特征的关系及对预后的影响。方法：选择2017年1月～2018年12月于内蒙古医科大学附属医院诊断并经手术切除治疗的HCC患者60例。采用实时荧光定量聚合酶链式反应（RT-qPCR）检测HCC组织、癌旁组织中GPSM2 、GFPT2 、SNORA51 mRNA表达情况，分析GPSM2、GFPT2 、SNORA51的mRNA表达与HCC患者临床病理特征的关系。随访3年，应用Kaplan-Meier生存曲线分析不同分组HCC患者预后情况，并应用单因素和多因素Logistic回归分析HCC患者预后的影响因素。结果：HCC组织中GPSM2、GFPT2 、SNORA51 mRNA表达水平显著高于癌旁组织（P<0.05）。HCC组织中GPSM2 mRNA高表达组、GFPT2 mRNA高表达组、SNORA51mRNA高表达组血管侵犯、TNM分期Ⅲ期比例显著高于GPSM2 mRNA低表达组、GFPT2 mRNA低表达组、SNORA51mRNA低表达组（P<0.05）。Kaplan-Meier法分析显示GPSM2 mRNA低表达组、GFPT2 mRNA低表达组、SNORA51mRNA低表达组3年生存率显著高于GPSM2 mRNA高表达组、GFPT2 mRNA高表达组、SNORA51mRNA高表达组（P<0.05）。多因素Logistic回归分析模型结果显示，血管侵犯、TNM分期Ⅲ期、GPSM2 mRNA高表达、GFPT2 mRNA高表达、SNORA51 mRNA高表达是HCC患者死亡的危险因素（P＜0.05）。结论：GPSM2 、GFPT2、SNORA51在HCC组织中异常高表达，且与血管侵犯、TNM分期等临床病理特征有关，GPSM2 、GFPT2 、SNORA51高表达是HCC患者死亡的危险因素。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between mRNA expression of G protein signal regulated protein 2 (GPSM2), glutamine fructose-6-phosphate transaminase 2 (GFPT2), nucleolar small RNA 51 (SNORA51) and clinicopathological features of hepatocellular carcinoma (HCC) tissues and their effects on prognosis. Methods: 60 patients with HCC who were diagnosed and treated with surgical resection in Affiliated Hospital of Inner Mongolia Medical University from January 2017 to December 2018 were selected. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expressions of GPSM2, GFPT2 and SNORA51 in HCC tissues and paracancer tissues, and the relationship between the mRNA expressions of GPSM2, GFPT2 and SNORA51 and the clinicopathological characteristics of patients with HCC were analyzed. After 3 years of follow-up, Kaplan-Meier survival curve was used to analyze the prognosis of patients with HCC in different groups, and univariate and multivariate Logistic regression was used to analyze the influencing factors of prognosis of patients with HCC. Results: The mRNA expression levels of GPSM2, GFPT2 and SNORA51 in HCC tissues were significantly higher than those in paracancer tissues (P<0.05). The proportion of vascular invasion and TNM stage Ⅲ in the GPSM2 mRNA high expression group, GFPT2 mRNA high expression group and SNORA51mRNA high expression group were significantly higher than those in the GPSM2 mRNA low expression group and GFPT2 mRNA low expression group and SNORA51mRNA low expression group (P<0.05). Kaplan-Meier analysis showed that the 3-year survival rate of GPSM2 mRNA low expression group, GFPT2 mRNA low expression group, SNORA51mRNA low expression group were significantly higher than those in the GPSM2 mRNA high expression group, GFPT2 mRNA high expression group and SNORA51mRNA high expression group (P<0.05). The results of multivariate Logistic regression analysis model showed that vascular invasion, TNM stage Ⅲ, GPSM2 mRNA high expression, GFPT2 mRNA high expression and SNORA51 mRNA high expression were the risk factors for death of patients with HCC (P<0.05). Conclusion: The abnormally high expression of GPSM2, GFPT2 and SNORA51 in HCC tissues is associated with clinicopathological features such as vascular invasion and TNM stage, and the high expression of GPSM2, GFPT2 and SNORA51 is a risk factor for death of patients with HCC. |
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