| 常子维,谈 捷,李秋红,唐海龙,张友山.BCR-ABL融合基因与免疫表型在急性B淋巴细胞白血病中的相关性研究[J].,2023,(1):137-141 |
| BCR-ABL融合基因与免疫表型在急性B淋巴细胞白血病中的相关性研究 |
| Correlation between BCR-ABL and Immunophenotype in Acute B Lymphocytic Leukemia |
| 投稿时间：2022-02-27 修订日期：2022-03-24 |
| DOI：10.13241/j.cnki.pmb.2023.01.027 |
| 中文关键词: 免疫表型 BCR-ABL 免疫分型亚型 |
| 英文关键词: Immunophenotype BCR-ABL Immunotyping subtype |
| 基金项目:国家自然科学基金项目（82100218） |
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| 中文摘要: |
| 摘要 目的：通过回顾性分析急性淋巴细胞白血病的免疫分型结果及融合基因表达情况，研究BCR-ABL融合基因与免疫表型的相关性。方法：用运SPSS23.0软件及流式分析软件KALUZA，分类比较BCR-ABL阳性和阴性病例中的免疫表型抗体阳性率及荧光强度，同时分析免疫表型亚型与年龄的关系。结果：在阳性率的比较中，CD10、CD34、CD25、TDT、CD38、IgM、CD45、CD303及免疫球蛋白轻链P＜0.05，存在统计学意义。在BCR-ABL阴性病例，免疫分型四种不同亚型在以18和40岁分界的三个年龄段的比较中，＜18和≥40的比较在Pre-B-ALL和普通B-ALL中存在差异，P＜0.05，有统计学意义；18-39和≥40的比较在普通B-ALL中存在差异，P＜0.05，有统计学意义。CD10、CD34、CD45的荧光强度在BCR-ABL阳性和阴性病例比较中存在差异，具有统计学意义。结论：BCR-ABL与免疫表型存在相关性，BCR-ABL阳性的急性淋巴细胞白血病免疫分型更倾向于表达幼稚细胞抗体，如CD10、CD34， BCR-ABL阴性病例在免疫表型亚型中主要为成熟B-ALL，更倾向于表达成熟抗体如膜免疫球蛋白KAPPA、LAMBDA。CD45荧光强度在BCR-ABL阴性病例中比阳性病例表达更强。 |
| 英文摘要: |
| ABSTRACT Objective: To study the correlation between BCR-ABL fusion gene and immunophenotype by retrospectively analyzing the results of immunophenotyping and fusion gene expression in acute lymphoblastic leukemia. Methods: Using SPSS23.0 software and flow analysis software KALUZA, the positive rate and fluorescence intensity of immunophenotype antibody in BCR-ABL positive and negative cases were classified and compared, and the relationship between immunophenotype and age was also analyzed. Results: In the comparison of positive rate, CD10, CD34, CD25, TDT, CD38, IgM, CD45, CD303 and immunoglobulin light chain P<0.05, there was statistical significance. In BCR-ABL negative cases, immunophenotyping of four different subtypes in three age groups divided by 18 and 40 years, comparison of <18 and ≥40 in Pre-B-ALL and Common B-ALL There is difference, P<0.05, there is statistical significance; 18-39 and ≥40 have differences in Common B-ALL, P<0.05, there is statistical significance. The fluorescence intensities of CD10, CD34 and CD45 were significantly different between BCR-ABL positive and negative cases. Conclusion: There is a correlation between BCR-ABL and immunophenotype. BCR-ABL positive acute lymphoblastic leukemia immunophenotypes are more inclined to express immature cell antibodies, such as CD10 and CD34, and BCR-ABL-negative cases are in the immunophenotype. Mainly mature B-ALL, more inclined to express mature antibodies such as membrane immunoglobulin KAPPA, LAMBDA. CD45 fluorescence intensity was stronger in BCR-ABL negative cases than in positive cases. |
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