| 邓 堂,钟士杰,刘华江,张名林,魏胜超,廖 勇,史键山,金桂云,吕鹏飞.车前草提取物对缺氧性肺动脉高压大鼠模型氧化应激的影响[J].,2023,(1):41-46 |
| 车前草提取物对缺氧性肺动脉高压大鼠模型氧化应激的影响 |
| Effect of Plantago Depressa Willd Extract on Oxidative Stress in a Rat Model of Hypoxic Pulmonary Hypertension |
| 投稿时间：2022-05-27 修订日期：2022-06-23 |
| DOI：10.13241/j.cnki.pmb.2023.01.008 |
| 中文关键词: 车前草提取物 缺氧性肺动脉高压 抗氧化 氧化应激 |
| 英文关键词: Extract of Plantago depressa Willd Hypoxic pulmonary hypertension Antioxidant Oxidative stress |
| 基金项目:海南省重点研发社会发展项目（ZDYF2022SHFZ293；国家自然科学基金项目（31560267）；海南省自然科学基金（820RC764） |
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| 中文摘要: |
| 摘要 目的：研究车前草提取物（PW）的抗氧化性对缺氧性肺动脉高压(HPH)大鼠模型氧化应激的影响，分析并测定PW中发挥抗氧化的主要成分。方法：随机选取18只SD 雄性大鼠分为3组：CONT组、HPH组、HPH+PW组。CONT组和HPH组予生理盐水灌胃，HPH+PW组予车前草提取物灌胃，连续灌胃4周，测定大鼠肺动脉压力（mPAP），肺功能相关指标（0.4 sFEV、FVC、 0.4sFEV/FVC、MMEF）后处死。试剂盒测定各组大鼠肺泡灌洗液中SOD、CAT、LDH、MDA含量。液相色谱-质谱分析鉴定PW中的有效成分并测定PW有效成分对超氧阴离子的清除能力。结果：HPH组与PW组相比，mPAP显著升高（**P＜0.01），0.4 sFEV、FVC、0.4sFEV/FVC、MMEF显著降低（**P＜0.01），HPH+PW可逆转上述趋势。HPH组与Cont组相比，SOD、CAT显著降低（**P＜0.01），LDH、MDA显著升高（**P＜0.01），HPH+PW可逆转上述趋势。液相色谱-质谱分析并鉴定出PW中含有车前子苷、高车前苷、β-谷甾醇、齐墩果酸、熊果酸、京尼平苷酸、桃叶珊瑚苷、葡萄糖8种有效成分，其中桃叶珊瑚苷的超氧阴离子清除能力最强。结论：PW可提高HPH大鼠抗氧化酶含量，减轻氧化应激反应并降低肺动脉压力，改善肺功能；PW有效成分中桃叶珊瑚苷（AU）的抗氧化能力最强。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of antioxidant properties of plantain extract (PW) on oxidative stress in a rat model of hypoxic pulmonary hypertension (HPH), and to analyze and determine the main components of PW that exert antioxidant effects. Methods: Eighteen SD male rats were randomly selected and divided into three groups: the CONT group, the HPH group, and the HPH+PW group. the CONT and HPH groups were gavaged with saline, and the HPH+PW group was gavaged with PW for 4 weeks.MPAP, pulmonary function-related indexes (0.4 sFEV, FVC, 0.4 sFEV/FVC, and MMEF) were measured in rats after gavage. MMEF) and then executed. The kits were used to determine the contents of SOD, CAT, LDH and MDA in the alveolar lavage fluid of rats in each group. Liquid chromatography-mass spectrometry (LC-MS) analysis was performed to identify the active ingredients in PW and to determine the scavenging ability of PW active ingredients on superoxide anion. Results: In the HPH group, mPAP was significantly higher (**P<0.01) and 0.4 sFEV, FVC, 0.4 sFEV/FVC, and MMEF were significantly lower (**P<0.01) compared with the PW group, and HPH+PW reversed these trends. mPAP was significantly lower (**P<0.01) and LDH and MDA were significantly higher (**P<0.01) compared with the Cont group. (**P<0.01), and HPH+PW could reverse the above trend. Liquid chromatography-mass spectrometry analysis and identification of PW contained eight active ingredients of psyllium, hypericin, β-sitosterol, oleanolic acid, ursolic acid, kynurenine, peach leaf coral glucoside, glucose, of which peach leaf coral glucoside had the strongest superoxide anion scavenging ability. Conclusion: PW can increase the content of antioxidant enzymes in HPH rats, reduce oxidative stress, reduce pulmonary artery pressure, and improve pulmonary function. Aucubin (AU) has the strongest antioxidant capacity among the active components of PW. |
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