| 吴惠林,汪 炜,张少君,葛 睿,俞文娟,金 蝉,周 芳,邢倩倩,梁 艳.环黄芪醇治疗白癜风的作用及机制[J].,2023,(1):35-40 |
| 环黄芪醇治疗白癜风的作用及机制 |
| The Effect and Mechanism of Cycloastragenol in the Treatment of Vitiligo |
| 投稿时间：2022-04-28 修订日期：2022-05-24 |
| DOI：10.13241/j.cnki.pmb.2023.01.007 |
| 中文关键词: 环黄芪醇 白癜风 黑素细胞 黑色素 p38信号通路 |
| 英文关键词: Cycloastragenol Vitiligo Melanocytes Melanin p38 signaling pathway |
| 基金项目:国家自然科学基金项目（81802727） |
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| 中文摘要: |
| 摘要 目的：探索环黄芪醇（Cycloastragenol，CAG）对白癜风的治疗作用及机制。方法：将正常人皮肤黑素细胞PIG1分组如下：对照组、H2O2组、10CAG组、50CAG组和100CAG组，分别使用不同浓度（10、50、100 μM）的环黄芪醇和250 μM的H2O2 与PIG1细胞共培养。通过CCK-8法测定细胞增殖，Annexin V-FITC/PI法检测细胞凋亡，并测定细胞中的黑色素、SOD、CAT和MDA含量。将C57BL/6小鼠随机分为对照组、模型组和CAG组。通过每天涂抹50 mg 40%的莫诺苯宗乳膏建立白癜风小鼠模型，然后使用50 mg/kg的环黄芪醇治疗小鼠。通过HE染色和Masson-Fontana染色检测皮肤组织中的毛囊和黑色素含量。通过Western blotting检测MITF、TYR、TRP-1、TRP-2、Bax、Bcl-2、p-p38/p38的蛋白表达。结果：与H2O2组相比，50CAG组和100CAG组的细胞活力和黑色素含量均升高，凋亡率均降低，Bax蛋白表达水平均降低，而Bcl-2均升高，SOD和CAT水平均升高，而MDA水平均降低（P<0.05）。10CAG组、50CAG组和100CAG组的毛囊和黑色素含量均较H2O2组增多。与H2O2组相比，50CAG组和100CAG组的MITF、TYR、TRP-1、TRP-2和p-p38/p38蛋白表达水平均升高（P<0.05）。与模型组相比，CAG组的的MITF、TYR、TRP-1、TRP-2和p-p38/p38蛋白表达水平均升高（P<0.05）。结论：环黄芪醇在细胞水平和动物水平上均能促进黑色素合成，对白癜风具有较好的治疗作用，其机制可能与p38信号通路的激活有关。 |
| 英文摘要: |
| ABSTRACT Objective: The purpose of this study was to explore the therapeutic effect and mechanism of cycloastragenol (CAG) on vitiligo. Methods: The normal human skin melanocytes PIG1 were divided into the following groups: control group, H2O2 group, 10CAG group, 50CAG group, 100CAG group. PIG1 cells were cultured with different concentrations (10, 50, 100 μM) of cycloastragenol and 250 μM of H2O2, respectively. Cell proliferation was detected by CCK-8 method, apoptosis was detected by Annexin V-FITC/PI method, and the contents of melanin, SOD, CAT and MDA in cells were determined. C57BL/6 mice were randomly divided into control group, Model group and CAG group. The vitiligo mouse model was established by applying 50 mg of 40% monobenzone cream daily, and then the mice were treated with 50 mg/kg cycloastragenol. Hair follicles and melanin content in skin tissue were detected by HE staining and Masson-Fontana staining. The protein expressions of MITF, TYR, TRP-1, TRP-2, Bax, Bcl-2, p-p38/p38 were detected by Western blotting. Results: Compared with the H2O2 group, the 50CAG group and 100CAG groups had increased cell viability and melanin content, decreased apoptosis rate, decreased Bax protein expression, and increased Bcl-2 protein expression, increased SOD and CAT levels, and decreased MDA levels (P<0.05). The content of hair follicles and melanin in 10CAG group, 50CAG group and 100CAG group were higher than those in H2O2 group. Compared with the H2O2 group, the protein expression levels of MITF, TYR, TRP-1, TRP-2 and p-p38/p38 in the 50CAG group and 100CAG group were all increased (P<0.05). Compared with the model group, the protein expression levels of MITF, TYR, TRP-1, TRP-2 and p-p38/p38 in the CAG group were all increased (P<0.05). Conclusion: Cycloastragenol can promote melanin synthesis at both cellular and animal levels, and has a good therapeutic effect on vitiligo. The mechanism may be related to the activation of p38 signaling pathway. |
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