| 程建岗,李伟华,孟国林,毕 龙,成千亮.circPPP1R12A激活p53信号通路调控骨关节炎中软骨细胞增殖和凋亡[J].,2023,(1):14-19 |
| circPPP1R12A激活p53信号通路调控骨关节炎中软骨细胞增殖和凋亡 |
| CircPPP1R12A Activates p53 Signaling Pathway to Regulate Chondrocyte Proliferation and Apoptosis in Osteoarthritis |
| 投稿时间：2022-07-10 修订日期：2022-08-07 |
| DOI：10.13241/j.cnki.pmb.2023.01.003 |
| 中文关键词: 骨关节炎 circPPP1R12A 增殖 凋亡 p53信号通路 |
| 英文关键词: Osteoarthritis circPPP1R12A Proliferation Apoptosis p53 signaling pathway |
| 基金项目:国家重点研发计划重点专项子课题（2016YFC1100300） |
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| 中文摘要: |
| 摘要 目的：探讨circPPP1R12A（circ_0000423）调控p53信号通路对骨关节炎（osteoarthritis，OA）中软骨细胞增殖和凋亡的影响。方法：采用qRT-PCR检测circPPP1R12A在OA软骨细胞中的表达水平。在OA软骨细胞中分别转染oe-circPPP1R12A和sh-circPPP1R12A后，采用CCK-8检测细胞增殖情况；免疫荧光检测Ki-67阳性细胞表达率；流式细胞术检测细胞凋亡情况；qRT-PCR检测Ki-67和p53表达水平；Western Blot检测Cleaved-caspase3、P53、BCL-2和BAX的表达水平。结果：OA软骨细胞中circPPP1R12A的表达水平明显高于正常软骨细胞。过表达circPPP1R12A能够抑制OA软骨细胞增殖和促进细胞凋亡，通过上调p53表达激活p53信号通路，低表达circPPP1R12A能够促进OA软骨细胞增殖和抑制细胞凋亡，通过下调p53表达阻滞p53信号通路。在OA软骨细胞中同时低表达circPPP1R12A和过表达p53能够反转单独低表达circPPP1R12A对OA软骨细胞增殖和凋亡的影响。结论：circPPP1R12A在OA软骨细胞中明显高表达，circPPP1R12A能够通过激活p53信号通路抑制骨OA软骨细胞增殖和促进软骨细胞凋亡。circPPP1R12A可能成为OA治疗的干预靶点。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of circPPP1R12A (circ_0000423) regulating p53 signaling pathway on chondrocyte proliferation and apoptosis in osteoarthritis (OA). Methods: The expression level of circPPP1R12A in OA chondrocytes was detected by qRT-PCR. After transfection of oe-circPPP1R12A and sh-circPPP1R12A in OA chondrocytes, CCK-8 was used to detect cell proliferation; immunofluorescence was used to detect the expression rate of Ki-67 positive cells; flow cytometry was used to detect cell apoptosis; qRT-PCR was used to detect the expression levels of Ki-67 and p53; Western Blot was used to detect the expression levels of Cleaved-caspase3, P53, BCL-2 and BAX. Results: The expression level of circPPP1R12A in OA chondrocytes was significantly higher than that in normal chondrocytes. Overexpression of circPPP1R12A inhibited OA chondrocyte proliferation and promoted apoptosis, and activated p53 signaling pathway by up-regulating p53 expression. Low expression of circPPP1R12A promoted OA chondrocyte proliferation and inhibited cell apoptosis, and downregulated p53 expression to block p53 signaling pathway. Simultaneous low expression of circPPP1R12A and overexpression of p53 in OA chondrocytes reversed the effects of low expression of circPPP1R12A alone on the proliferation and apoptosis of OA chondrocytes. Conclusion: circPPP1R12A was significantly highly expressed in OA chondrocytes, and circPPP1R12A inhibited the proliferation of bone OA chondrocytes and promoted chondrocyte apoptosis by activating the p53 signaling pathway. circPPP1R12A may become an intervention target for OA treatment. |
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