| 杨 勇,王英男,全 超,丁苏宁,杨文霞,宋明旭.不同程度精索静脉曲张对不育男性精浆生化标志物及精子DNA完整性的影响[J].,2022,(24):4738-4741 |
| 不同程度精索静脉曲张对不育男性精浆生化标志物及精子DNA完整性的影响 |
| Effects of Varying Levels of Varicocele on Seminal Plasma Biochemical Markers and Sperm DNA Integrity in Infertile Men |
| 投稿时间：2022-05-27 修订日期：2022-06-23 |
| DOI：10.13241/j.cnki.pmb.2022.24.027 |
| 中文关键词: 精索静脉曲张 男性不育症 精浆α-葡萄糖苷酶 精子DFI |
| 英文关键词: Varicocele Male infertility Seminal plasma α-glucosidase DNA fragmentation index |
| 基金项目:国家自然科学基金重大研究计划项目(92068109) |
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| 中文摘要: |
| 摘要 目的：探讨不同程度精索静脉曲张（varicocele，VC）对不育男性精浆生化标志物及精子DNA完整性的影响。方法：选取2017年8月至2020年9月至我院生殖医学中心男性专科门诊就诊并进行精浆生化及精子DNA完整性检查的不育患者138例，按照有无VC分为VC不育组62例，非VC不育组76例，选取同期就诊无VC的正常生育者60例作为本研究的对照组，根据精索静脉曲张程度将VC不育组患者分为VCⅠ度组、VCⅡ度组、VCⅢ度组。比较VC不育组、非VC不育组与正常生育组精浆生化标志物及精子DNA碎片率（DNA fragmentation index，DFI），分析比较VC不育各亚组间精浆生化标志物及精子DFI变化情况。结果：精浆生化标志物分析：VC不育组精浆α-葡萄糖苷酶较非VC不育组及正常生育组明显减低（P＜0.05），且VC不育各亚组间精浆α-葡萄糖苷酶水平依次减低，三组间两两比较有显著性差异（P＜0.05）；精浆锌、柠檬酸、果糖在VC不育组、非VC不育组与正常生育组比较无统计学差异（P＞0.05），在VC不育各亚组间比较均无显著性差异（P＞0.05）；精子DFI分析：VC不育组精子DFI较正常生育组升高（P＜0.05），非VC不育组精子DFI较正常生育组升高（P＜0.05），VC不育组精子DFI较非VC不育组比较未见明显差异（P＞0.05）；VC不育各亚组间精子DFI逐渐升高，三组间两两比较精有显著性差异（P＜0.05）。结论：VC的发生与进展程度与精子DFI及精浆α-葡萄糖苷酶水平异常具有相关性，其二者可作为评价VC的有效指标，并为VC的分度提供一定的依据。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects of varying levels of Varicocele (VC) on seminal plasma biochemical markers and sperm DNA integrity in infertile men. Methods: A total of 138 infertile patients were enrolled from August 2017 to September 2020, who were treated in the male clinic of Reproductive Medicine Center of our hospital and had their seminal plasma biochemistry and sperm DNA integrity, all cases are divided into 62 VC infertility group and 76 non-VC infertility group, and at the same time 60 normal men without VC were selected as normal control. Patients with VC infertility were divided into VCI group, VCII group and VCIII group according to the levels of varicocele. The changes of seminal plasma biochemical markers and sperm DNA fragmentation index (DFI) were compared between VC sterility group, non-VC sterility group and normal fertility group. Results: The α-glucosidase in VC sterile group was significantly lower than that in non-VC sterile group and normal fertile group (P<0.05), and the level of α-glucosidase in seminal plasma decreased in different subgroups of VC sterility, and there was significant difference between two groups (P<0.05). There was no significant difference (P>0.05) in zinc, citric acid and fructose in seminal plasma between VC sterile group and non-VC sterile group and normal fertile group, DFI analysis showed that DFI of sperm in VC infertility group was significantly higher than that in normal fertility group (P<0.05), and DFI of sperm in non-VC infertility group was higher than that in normal fertility group (P<0.05) , there was no significant difference in DFI of sperm between VC infertility group and non-VC infertility group (P>0.05), but DFI of sperm in VC infertility sub-group increased gradually, and there was significant difference between two groups (P<0.05). Conclusion: The occurrence and progression of VC is correlated with abnormal DFI in spermatozoa and α-glucosidase in seminal plasma levels, which can be used as effective indicators for VC evaluation and provide a certain basis for VC grading. |
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