| 周炜华,牛成山,邸平伟,冯启深,李家雪.阿尔茨海默病血清miR-137、miR-138表达与认知功能损害和外周血淋巴细胞PI3K/Akt信号通路的关系研究[J].,2022,(24):4674-4678 |
| 阿尔茨海默病血清miR-137、miR-138表达与认知功能损害和外周血淋巴细胞PI3K/Akt信号通路的关系研究 |
| Relationship Study between Serum miR-137, miR-138 Expression and Cognitive Impairment and PI3K/Akt Signaling Pathway in Peripheral Blood Lymphocytes in Alzheimer's Disease |
| 投稿时间：2022-05-23 修订日期：2022-06-18 |
| DOI：10.13241/j.cnki.pmb.2022.24.014 |
| 中文关键词: 阿尔茨海默病 miR-137 miR-138 认知功能 PI3K/Akt信号通路 |
| 英文关键词: Alzheimer's disease miR-137 miR-138 Cognitive function PI3K/Akt signaling pathway |
| 基金项目:山东省医药卫生科技发展计划项目（2017DX0053） |
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| 中文摘要: |
| 摘要 目的：探讨阿尔茨海默病（AD）患者血清微小核糖核酸（miR）-137、miR-138表达与认知功能损害和外周血淋巴细胞磷脂酰肌醇-3激酶/蛋白激酶（PI3K/Akt）信号通路的关系。方法：选取2020年1月至2022年5月青岛大学附属医院神经内科收治的95例AD患者（AD组），根据临床痴呆评定量表（CDR）评分将患者分为轻度组（1分，35例）、中度组（2分，42例）、重度组（3分，18例），另选取63例体检健康志愿者为对照组。检测AD组、对照组血清miR-137、miR-138表达水平以及外周血淋巴细胞PI3K/Akt信号通路相关蛋白表达，采用简易智能精神状态检查量表（MMSE）、蒙特利尔认知评估量表（MoCA）评估认知功能。分析血清miR-137、miR-138与MMSE、MoCA评分以及外周血淋巴细胞PI3K、Akt、B淋巴细胞瘤-2基因（Bcl-2）、Bcl-2相关X蛋白基因（Bax）表达的相关性。结果：AD组血清miR-137水平、外周血淋巴细胞PI3K、Akt、Bcl-2蛋白表达水平及MMSE、MoCA评分低于对照组（P＜0.05），血清miR-138、外周血淋巴细胞Bax蛋白表达水平高于对照组（P＜0.05）。重度组血清miR-137水平、外周血淋巴细胞PI3K、Akt、Bcl-2蛋白表达水平及MMSE、MoCA评分低于中度组和轻度组（P＜0.05），且中度组低于轻度组（P＜0.05）；重度组血清miR-138、外周血淋巴细胞Bax蛋白表达水平高于中度组和轻度组（P＜0.05），且中度组高于轻度组（P＜0.05）。AD患者血清miR-137水平与MMSE、MoCA评分、外周血淋巴细胞PI3K、Akt、Bcl-2蛋白表达呈正相关（P＜0.05），与外周血淋巴细胞Bax蛋白表达呈负相关（P＜0.05）；AD患者血清miR-138水平与MMSE、MoCA评分、外周血淋巴细胞PI3K、Akt、Bcl-2蛋白表达呈负相关（P＜0.05），与外周血淋巴细胞Bax蛋白表达呈正相关（P＜0.05）。结论：AD患者的血清miR-137表达水平降低、miR-138表达水平增高，与认知功能障碍有关，且miR-137、miR-138可能通过调控PI3K/Akt信号通路参与AD发病过程。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between serum microribonucleic acid (miR)-137 and miR-138 expression and cognitive impairment and phosphatidylinositol 3 kinase/protein kinase (PI3K/Akt) signaling pathway in peripheral blood lymphocytes in patients with Alzheimer's disease (AD). Methods: From January 2020 to May 2022, 95 patients wth AD (AD group) who were admitted to the Department of Internal Medicine Neurology of the Affiliated Hospital of Qingdao University were selected. According to the clinical dementia rating scale (CDR) score, the patients were divided into mild group (1 scores, 35 cases), moderate group (2 scores, 42 cases), severe group (3 scores, 18 cases), and 63 healthy volunteers were selected as the control group. The levels of serum miR-137, miR-138 expression and the expression of PI3K/Akt signal pathway related proteins in peripheral blood lymphocytes of AD group and control group were detected. The cognitive function was evaluated with the Mini Mental State Examination (MMSE) and Montreal Cognitive Assessment Scale (MoCA). The correlation between serum miR-137, miR-138 and MMSE, MoCA score, as well as the expression of PI3K, Akt, B lymphomatoma-2 gene (Bcl-2), Bcl-2 related X protein gene (Bax) in peripheral blood lymphocytes were analyzed. Results: The levels of serum miR-137 and PI3K, Akt and Bcl-2 protein expression in peripheral blood lymphocytes, MMSE and MoCA scores in the AD group were lower than those in the control group (P＜0.05), while the levels of serum miR-138 and Bax protein expression in peripheral blood lymphocytes were higher than those in the control group (P＜0.05). The levels of serum miR-137 and PI3K, Akt and Bcl-2 protein expression in peripheral blood lymphocytes, and the scores of MMSE and MoCA in the severe group were lower than those in the moderate group and mild group (P＜0.05), and the moderate group was lower than the mild group (P＜0.05). The levels of miR-138 and Bax protein expression in peripheral blood lymphocytes were higher than those in the moderate group and mild group (P＜0.05), and the moderate group was higher than the mild group (P＜0.05). Serum miR-137 level in patients wth AD was positively correlated with MMSE, MoCA score, PI3K, Akt and Bcl-2 protein expression in peripheral blood lymphocytes (P＜0.05), and negatively correlated with Bax protein expression in peripheral blood lymphocytes (P＜0.05). Serum miR-138 level in patients wth AD was negatively correlated with MMSE, MoCA score, PI3K, Akt and Bcl-2 protein expression in peripheral blood lymphocytes (P＜0.05), and positively correlated with Bax protein expression in peripheral blood lymphocytes (P＜0.05). Conclusion: The serum miR-137 expression is decreased and miR-138 is increased in the patients wth AD, which is related to cognitive dysfunction. miR-137 and miR-138 may be involved in the pathogenesis of AD by regulating PI3K/Akt signaling pathway. |
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