文章摘要
徐 望,赵 琴,邓廷超,杨 亚,张尧尧.牙龈卟啉单胞菌感染对慢性牙周炎患者牙周指标、NLRP-3炎性小体通路和Th17/Treg细胞平衡的影响[J].,2022,(23):4555-4559
牙龈卟啉单胞菌感染对慢性牙周炎患者牙周指标、NLRP-3炎性小体通路和Th17/Treg细胞平衡的影响
Effects of Porphyromonas Gingivalis Infection on Periodontal Indexes, NLRP-3 Inflammasome Pathway and Th17/Treg Cell Balance in Patients with Chronic Periodontitis
投稿时间:2022-03-18  修订日期:2022-04-12
DOI:10.13241/j.cnki.pmb.2022.23.031
中文关键词: 慢性牙周炎  牙龈卟啉单胞菌  Nod样受体蛋白-3  辅助性T细胞17  调节性T细胞
英文关键词: Chronic periodontitis  Porphyromonas gingivalis  Nod like receptor protein-3  T-helper cell 17  T-regulatory cell
基金项目:重庆市医学科研计划项目(2018MSXM1142)
作者单位E-mail
徐 望 重庆医科大学附属巴南医院口腔科 重庆 401320 xw736419953@163.com 
赵 琴 重庆医科大学附属巴南医院口腔科 重庆 401320  
邓廷超 重庆医科大学附属巴南医院口腔科 重庆 401320  
杨 亚 重庆医科大学附属巴南医院口腔科 重庆 401320  
张尧尧 重庆医科大学附属巴南医院口腔科 重庆 401320  
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中文摘要:
      摘要 目的:探讨牙龈卟啉单胞菌(PG)感染对慢性牙周炎(CP)患者牙周指标、Nod样受体蛋白-3(NLRP-3)炎性小体通路和辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的影响。方法:选择2018年6月至2021年11月在本院进行拔牙治疗的慢性牙周炎患者106例为CP组,根据是否感染PG分为感染组和未感染组;另选择因阻生齿、错位牙在本院进行治疗的牙周健康患者63例为对照组。记录所有对象的探诊深度(PD)、菌斑指数(PLI)、出血指数(BI)、附着丧失(AL),采用聚合酶链式反应(PCR)技术检测PG感染率,采用RT-PCR技术检测牙周膜组织中NLRP-3 mRNA,接头蛋白凋亡相关斑点样蛋白(ASC) mRNA、半胱氨酸天冬氨酸酶-1(Caspase-1) mRNA的表达量,采用FACSCalibur流式细胞仪检测外周血Th17、Terg 水平并计算Th17/Treg,比较两组各检测指标水平。结果:对照组PG感染阳性率为17.46%,低于CP组的79.25%,两组比较差异有统计学意义(P<0.05)。感染组PD、PLI、BI、AL水平均高于非感染组,差异有统计学意义(P<0.05)。感染组NLRP-3 mRNA、ASC mRNA、Caspase-1 mRNA表达量均高于非感染组,差异有统计学意义(P<0.05)。感染组外周血Th17细胞、Th17/Treg水平高于非感染组,Treg水平低于非感染组,差异有统计学意义(P<0.05)。结论:CP患者多数存在PG感染,PG感染对患者的牙周健康、炎症及免疫有明显的影响。
英文摘要:
      ABSTRACT Objective: To investigate the effects of porphyromonas gingivalis (PG) infection on periodontal indexes, nod like receptor protein-3 (NLRP-3) and T-helper cell 17 (Th17) / T-regulatory cell (Treg) balance in patients with chronic periodontitis (CP). Methods: 106 patients with chronic periodontitis who underwent tooth extraction in our hospital from June 2018 to November 2021 were selected as CP Group, they were divided into infection group and non-infection group according to whether they were infected with PG. 63 healthy periodontal patients who treated in our hospital due to impacted teeth and misplaced teeth were selected as the control group. The probing depth (PD), plaque index (PLI), bleeding index (BI), attachment loss (AL) of all objects were recorded, the infection rate of PG was detected by polymerase chain reaction (PCR), the expression of NLRP-3 mRNA, apoptosis-associated speck-like protein containing a CARD (ASC) mRNA and caspase-1 mRNA in periodontal ligament were detected by RT-PCR, the levels of Th17 and Terg in peripheral blood were detected by FACScalibur flow cytometry and Th17 / Treg was calculated, the levels of detection indexes in the two groups were compared. Results: The positive rate of PG infection in the control group was 17.46%, which was lower than 79.25% in the CP Group, the difference between the two groups was statistically significant(P<0.05). The levels of PD, PLI, BI, Al in the infection group were higher than those in the non-infection group, the differences were statistically significant(P<0.05). The expressions of NLRP-3 mRNA, ASC mRNA, Caspase-1 mRNA in the infection group were higher than those in the non-infection group, the differences were statistically significant(P<0.05). The levels of Th17 cells and Th17 / Treg in peripheral blood in the infection group were higher than those in the non-infection group, and the level of Treg in the infection group was lower than that in the non-infection group, the differences were statistically significant(P<0.05). Conclusion: Most patients with CP have PG infection, PG infection has a significant impact on periodontal health, inflammation and immunity of patients with CP.
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