| 盛春瑞,刘珊珊,陈以文,张 迪,宗晨钟,杜庆红,仁青加,王珊珊,董瑞娟,葛东宇,王淑艳,李丽娜.炙甘草汤通过抗氧化抑制博莱霉素诱导的小鼠肺纤维化[J].,2022,(21):4014-4019 |
| 炙甘草汤通过抗氧化抑制博莱霉素诱导的小鼠肺纤维化 |
| Zhigancao Decoction Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice through Antioxidation |
| 投稿时间：2022-02-27 修订日期：2022-03-23 |
| DOI：10.13241/j.cnki.pmb.2022.21.003 |
| 中文关键词: 特发性肺纤维化 炙甘草汤 氧化应激 |
| 英文关键词: Idiopathic pulmonary fibrosis Zhigancao decoction Oxidative stress |
| 基金项目:西藏自治区重点研发计划项目（XZ202001ZY0001G）；西藏自治区藏医药管理局级科技专项（JJKT2020006） |
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| 中文摘要: |
| 摘要 目的：探讨炙甘草汤对特发性肺纤维化（Idiopathic pulmonary fibrosis，IPF）小鼠纤维化相关指标的影响，挖掘炙甘草汤治疗IPF的机制。方法：将60只SPF级ICR小鼠随机分为空白组、模型组、吡菲尼酮组和炙甘草汤组，除空白组外，其余组采用气管滴注博莱霉素（5 mg/kg)方法复制IPF小鼠模型，并给予相应的药物治疗。空白组和模型组小鼠灌胃生理盐水，吡菲尼酮组和炙甘草汤组小鼠分别灌胃吡菲尼酮(50 mg/kg)和炙甘草汤（25.4 g/kg），各组均连续给药4周后取材，记录各组小鼠的死亡情况，计算各组肺系数；观察肺组织切片病理变化；碱水解法检测肺组织羟脯氨酸（HYP）含量；比色法检测肺组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性；免疫组化、荧光定量PCR检测α-SMA、COL1A蛋白和mRNA的表达水平。结果：炙甘草汤组小鼠死亡数减少，肺系数显著降低（P＜0.01），炎性细胞浸润和胶原沉积面积大量减少，肺泡结构逐渐修复，HYP、MDA含量降低（P＜0.01），SOD活性（P＜0.05）和GSH-Px活性（P＜0.01）显著增强；α-SMA、COL1A蛋白和mRNA表达均降低（P＜0.01）。结论：炙甘草汤通过抑制氧化应激反应，从而抑制成纤维细胞活化，减少细胞质基质沉积，从而减缓IPF疾病进程。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of Zhigancao decoction on fibrosis related indexes in mice with idiopathic pulmonary fibrosis (IPF), and to explore the mechanism of Zhigancao decoction in the treatment of IPF. Methods: 60 SPF ICR mice were divided randomly into blank group, model group, pirfenidone group and Zhigancao decoction group. In addition to the blank group, the other groups were treated with bleomycin (5 mg/kg) by intratracheal drip, and the corresponding drug treatment was given: the blank group and model group were given normal saline by gavage. Mice in pifenidone group and Zhigancao decoction group were given pifenidone (50 mg/kg) and Zhigancao decoction (25.4 g/kg) by gavage, respectively. After 4 weeks of continuous administration, the death of mice in each group was recorded and the lung coefficient of each group was calculated; The pathological changes of lung tissue sections were observed; The content of hydroxyproline (HYP) in lung tissue was detected by alkaline hydrolysis method; The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in lung tissue were detected by colorimetry; The expression of α-SMA, COL1A protein and mRNA was detected by immunohistochemistry and fluorescence quantitative PCR. Results: The death number of mice in Zhigancao decoction group decreased and the lung coefficient decreased significantly (P<0.01), the inflammatory cell infiltration and collagen deposition area decreased significantly, the alveolar structure was repaired gradually, the contents of HYP and MDA decreased (P<0.01), and the activities of SOD (P<0.05) and GSH-PX (P<0.01) increased; The expression of α-SMA, COL1A protein and mRNA decreased (P<0.01). Conclusion: Zhigancao decoction can inhibit the activation of fibroblasts and reduce the deposition of cytoplasmic matrix by inhibiting oxidative stress, so as to slow down the process of IPF disease. |
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