| 郭芝亮,施 全,季 冰,詹望桃,叶素琪.宫颈低级别鳞状上皮内病变组织CK8、P53及Ki-67的表达及其临床意义[J].,2022,(19):3780-3784 |
| 宫颈低级别鳞状上皮内病变组织CK8、P53及Ki-67的表达及其临床意义 |
| Expression and Clinical Significance of CK8, P53 and Ki-67 in Cervical Low-Grade Squamous Intraepithelial Lesion |
| 投稿时间：2022-04-23 修订日期：2022-05-18 |
| DOI：10.13241/j.cnki.pmb.2022.19.036 |
| 中文关键词: 宫颈低级别鳞状上皮内病变 CK8 P53 Ki-67 免疫组化 |
| 英文关键词: Cervical low-grade squamous intraepithelial lesion CK8 P53 Ki-67 Immunohistochemistry |
| 基金项目:广东省医学科学技术研究基金项目（C2021093） |
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| 中文摘要: |
| 摘要 目的：探讨宫颈低级别鳞状上皮内病变（LSIL）组织中细胞角蛋白 8（CK8）、P53及Ki-67的表达，分析其与病情进展的关系。方法：回顾性分析2020年1月至2021年12月我院收治的120例经病理确诊为LSIL患者的临床资料，根据末次随访病理学检查结果将其分为进展组（17例）、持续组（27例）和消退组（76例）。取首次活检宫颈组织标本，采用免疫组化法检测CK8、P53、Ki-67表达。多因素Logistic回归分析LSIL患者疾病进展的危险因素，受试者工作特征（ROC）曲线分析CK8、P53、Ki-67评估LSIL患者疾病进展的效能。结果：进展组宫颈组织中CK8、P53及Ki-67阳性表达率高于持续组和消退组（P＜0.05），持续组宫颈组织中CK8、P53及Ki-67阳性表达率高于消退组（P＜0.05）。高危人乳头瘤病毒（HPV）感染、CK8阳性细胞占比（较高）、P53阳性细胞占比（较高）、Ki-67阳性细胞占比（较高）是LSIL患者疾病进展的危险因素（P＜0.05）。联合CK8、P53、Ki-67阳性细胞占比预测LSIL患者疾病进展的曲线下面积为0.846，高于单独指标预测的0.637、0.697、0.744。结论：LSIL进展宫颈组织中CK8、P53、Ki-67阳性表达率明显升高，CK8、P53、Ki-67阳性细胞占比升高增加了LSIL患者疾病进展的风险，可作为辅助评估 LSIL进展的生物学指标。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of cytokeratin 8 (CK8), P53 and Ki-67 in cervical low-grade squamous intraepithelial lesion (LSIL), and to analyze their relationship with the disease progression. Methods: The clinical data of 120 patients with LSIL who were diagnosed by pathology in our hospital from January 2020 to December 2021 were analyzed retrospectively, according to the pathological results of the last follow-up, they were divided into progressive group (17 cases), continuous group (27 cases) and regression group (76 cases). The first biopsy of cervical tissue samples were taken, the expression of CK8, P53 and Ki-67 was detected by immunohistochemistry. Multivariate Logistic regression analysis was used to analyze the risk factors of disease progression in patients with LSIL. The efficacy of CK8, P53 and Ki-67 in evaluating disease progression in patients with LSIL was analyzed by receiver operating characteristic (ROC) curve. Results: The positive expression rates of CK8, P53 and Ki-67 in cervical tissue of progressive group were higher than those of continuous group and regression group(P＜0.05), and the positive expression rates of CK8, P53 and Ki-67 in cervical tissue of continuous group were higher than those of regression group (P＜0.05). High risk human papillomavirus (HPV) infection, proportion of CK8 positive cells (higher), proportion of P53 positive cells (higher) and proportion of Ki-67 positive cells (higher) were risk factors for disease progression in patients with LSIL(P＜0.05). The area under curve of combining CK8, P53 and Ki-67 positive cells to predict the disease progression of patients with LSIL was 0.846, which was higher than 0.637, 0.697 and 0.744 predicted by individual indicators. Conclusion: The positive expression rates of CK8, P53 and Ki-67 in cervical tissues are significantly increased, and the increased proportion of CK8, P53 and Ki-67 positive cells increased the risk of disease progression in patients with LSIL, which could be used as a biological indicator to assist the evaluation of LSIL progression. |
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