| 王 宁,郭春霞,高新茹,陈晓媛,彭艳侠,姚 彦.超声成像用于早期评估大鼠上皮性卵巢癌对多西他赛反应的动物实验[J].,2022,(18):3434-3439 |
| 超声成像用于早期评估大鼠上皮性卵巢癌对多西他赛反应的动物实验 |
| Ultrasound Imaging is Used in Animal Experiments to Assess the Response of Rat Epithelial Ovarian Cancer to Docetaxel at an Early Stage |
| 投稿时间：2022-03-27 修订日期：2022-04-23 |
| DOI：10.13241/j.cnki.pmb.2022.18.006 |
| 中文关键词: 超声成像 上皮性卵巢癌 多西他赛 早期评估 |
| 英文关键词: Ultrasound imaging Epithelial ovarian cancer Docetaxel Early evaluation |
| 基金项目:陕西省重点研发计划项目（2021SF-312） |
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| 中文摘要: |
| 摘要 目的：探究超声成像用于早期评估大鼠上皮性卵巢癌对多西他赛反应的可行性。方法：采用7,12-二甲基苯并[a]蒽，在Sprague Dawley大鼠中诱导原位上皮性卵巢癌（EOC）。筛选患有EOC的大鼠，在第0天使用多西他赛（DXT）治疗。在第0天，第3天，第7天，第14天和第21天应用CEUS评估治疗的早期反应。在第21天，治疗的肿瘤类型根据其大小的变化分为敏感和不敏感组，并进行组织病理学分析。结果：第3天以来上升时间（RT）、峰值强度（PI）、曲线下面积（AUC）的百分比变化与第14天肿瘤大小均存在显著差异（P＜0.05）。在治疗的早期阶段（第3天），RT与PI组合的百分比变化的灵敏度和特异性分别为100 %和100 %，使用RT与PI减少17.59 %的临界值。从第7天开始，敏感组与不敏感组相比，血管生成的定量指数百分比变化有显著差异（P＜0.05）。RT、PI和AUC的百分比变化与肿瘤大小和血管生成的变化百分比呈正相关，与坏死变化的百分比呈负相关（P＜0.05）。结论：CEUS参数在检测肿瘤组织病理学反应方面优于成像肿瘤大小，其在早期评估EOC中对DXT的反应具有显著优势，而在临床中，CEUS参数检测可能有助于在EOC进展或治疗过程中，更快地评估并调整无反应者的治疗方案，并改善其预后，因此在具有重要应用价值。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the feasibility of ultrasound imaging for early assessment of rat epithelial ovarian cancer response to docetaxel. Methods: Epithelial ovarian carcinoma (EOC) in situ was induced in Sprague Dawley rats using 7,12-dimethylbenzo[a]anthracene. Rats with EOC were screened and treated with docetaxel (DXT) on day 0. CEUS is applied on days 0, 3, 7, 14 and 21 to assess early response to treatment. On day 21, the tumor type treated is divided into sensitive and insensitive groups according to changes in their size, and histopathological analysis is performed. Results: The percentage change in rise time (RT), peak intensity (PI), and area under the curve (AUC) since day 3 was significantly different from the tumor size on day 14(P＜0.05). In the early stages of treatment (day 3), the sensitivity and specificity of the percentage change in the combination of RT and PI were 100 % and 100%, respectively, with a cut-off value of 17.59 % reduced with RT versus PI. From day 7 onwards, there was a significant difference in the percentage change in the quantitative index of angiogenesis in the sensitive group compared to the insensitive group(P＜0.05). Percentage changes in RT, PI, and AUC were positively correlated with the percentage change in tumor size and angiogenesis, and negatively associated with the percentage change in necrosis(P＜0.05). Conclusion: CEUS parameters are superior to imaging tumor size in detecting tumor histopathological response, which has a significant advantage in early assessment of response to DXT in EOC. In the clinic, CEUS parameter detection may help to evaluate and adjust the treatment plan of non-responders more quickly and improve their prognosis in the process of EOC progression or treatment, so it has important application value in the process of EOC. |
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