| 郭玉冰,王 潇,王 芯,李 星,李颖慧.IL-6抗体对银屑病小鼠的治疗作用及其对外周血辅助性T细胞和树突状细胞影响的研究[J].,2022,(15):2836-2840 |
| IL-6抗体对银屑病小鼠的治疗作用及其对外周血辅助性T细胞和树突状细胞影响的研究 |
| Therapeutic Effect of IL-6 Antibody on Psoriatic Mice and its Effect on Peripheral Blood Helper T Cells and Dendritic Cells |
| 投稿时间：2022-03-08 修订日期：2022-03-31 |
| DOI：10.13241/j.cnki.pmb.2022.15.007 |
| 中文关键词: IL-6 银屑病 树突状细胞 辅助性T细胞 STAT3 炎症 |
| 英文关键词: IL-6 Psoriasis Dendritic cells T helper cells STAT3 Inflammation |
| 基金项目:陕西省自然科学基础研究计划项目（2021JM-572） |
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| 中文摘要: |
| 摘要 目的：研究白介素-6（Interleukin-6，IL-6）抗体对银屑病小鼠的治疗作用，并探讨IL-6抗体治疗对银屑病小鼠外周血辅助性T细胞和树突状细胞影响。方法：30只6-8周龄SPF级BALB/c小鼠按照随机数字表法分为Normal组、Model组和IL-6组，Model组和IL-6组小鼠通过在背部脱毛区涂抹咪喹莫特建立银屑病小鼠模型。IL-6组小鼠通过在背部脱毛区皮下注射IL-6抗体进行治疗。比较三组小鼠皮损组织银屑病皮损面积及严重程度指数（psoriasis area and severity index,PASI）、表皮厚度和血管数、血清TNF-α、IL-17和IL-23含量、外周血树突状细胞、Th17细胞和Th22细胞比例以及皮损组织IL-6、IL-21和STAT3蛋白表达。结果：IL-6抗体治疗后，Model组和IL-6组小鼠皮损组织PASI、表皮厚度和血管数，血清TNF-α、IL-17和IL-23含量，外周血树突状细胞、Th17细胞和Th22细胞比例，以及皮损组织IL-6、IL-21和STAT3蛋白表达均显著高于Normal组小鼠（P<0.05）；与Model组相比，IL-6组小鼠皮损组织PASI、表皮厚度和血管数，血清TNF-α、IL-17和IL-23含量，外周血树突状细胞、Th17细胞和Th22细胞比例，以及皮损组织IL-6、IL-21和STAT3蛋白表达均显著降低（P<0.05）。结论：IL-6抗体对银屑病小鼠模型具有较好的治疗作用，其机制可能与通过抑制IL-6/STAT3通路影响Th17/Th22和树突状细胞水平有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the therapeutic effect of IL-6 antibody on psoriatic mice, and to explore the effect of IL-6 antibody treatment on peripheral blood helper T cells and dendritic cells of psoriatic mice. Methods: Thirty 6-8 week old SPF grade BALB/c mice were divided into Normal group, Model group and IL-6 group according to the random number table method. The Model group and IL-6 group were used to establish psoriasis mouse models by applying imiquimod. Mice in the IL-6 group were treated by subcutaneous injection of IL-6 antibody in the dorsal alopecia. The psoriasis area and severity index (PASI), epidermal thickness and number of blood vessels in the skin lesions of the three groups of mice were compared, serum TNF-α, IL-17 and IL-23 levels, peripheral blood dendritic cells, Th17 cells and Th22 cells ratios and IL-6, IL-21 and STAT3 protein expressions in skin lesions. Results: After IL-6 antibody treatment, PASI, epidermal thickness and blood vessel number, serum TNF-α, IL-17 and IL-23 contents, peripheral blood dendritic cells and Th17 cells of mice in Model group and IL-6 group were compared. The proportions of IL-6, IL-21 and STAT3 in skin lesions were significantly higher than those in the Normal group (P<0.05); compared with the Model group, the PASI in the skin lesions of the IL-6 group mice , epidermal thickness and blood vessel number, serum TNF-α, IL-17 and IL-23 levels, peripheral blood dendritic cells, Th17 cells and Th22 cell ratios, and IL-6, IL-21 and STAT3 protein expressions in skin lesions were significantly decreased(P<0.05). Conclusion: IL-6 antibody has a good therapeutic effect on the psoriasis mouse model, and its mechanism may be related to the influence of Th17/Th22 and dendritic cell levels by inhibiting the IL-6/STAT3 pathway. |
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