| 依力哈木·买买提,依马木买买提江·阿布拉,肖开提·阿不都哈德尔,杨 鹏,钟 锴.微小RNA-451a对胰腺癌BxPc3细胞增殖、侵袭及迁移的影响[J].,2022,(12):2217-2221 |
| 微小RNA-451a对胰腺癌BxPc3细胞增殖、侵袭及迁移的影响 |
| Effect of microRNA-451a on the Proliferation, Invasion and Migration of Pancreatic Cancer BxPc3 Cells |
| 投稿时间：2022-01-10 修订日期：2022-01-31 |
| DOI：10.13241/j.cnki.pmb.2022.12.004 |
| 中文关键词: miR-451a BxPc3 胰腺癌 增殖 侵袭 迁移 |
| 英文关键词: miR-451a BxPc3 Pancreatic cancer Proliferation Invasion Migration |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2017D01C305） |
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| 中文摘要: |
| 摘要 目的：研究微小RNA（miR）-451a对胰腺癌BxPc3细胞增殖、侵袭和迁移的影响。方法：体外培养BxPc3细胞，将不同浓度（50、100 和 200 μmol/L）miR-451a模拟物（mimic）转染至胰腺癌细胞株BxPc3中，利用荧光定量聚合酶链式反应（ PCR）法检测miR-451a的表达，MTT增殖实验检测不同浓度miR-451a对细胞增殖的影响，细胞划痕实验检测不同浓度miR-451a对细胞迁移能力的影响，Transwell实验检测不同浓度miR-451a对细胞侵袭能力的影响。Western blot法检测不同浓度 miR-451a转染后BxPc3细胞p-PI3K、p-AKT、TGF-β和p-smad2/3 蛋白的表达情况。结果：转染不同浓度miR-451a mimic后，胰腺癌BxPc3细胞内miR-451a的相对表达量明显升高，差异有统计学意义（P<0.05）。过表达miR-451a后，BxPc3细胞的增殖能力明显减弱，细胞迁移能力明显减弱，细胞侵袭能力明显减弱，差异均有统计学意义（P<0.05）。相比于0 μmol/L 组，转染50、200 μmol/L miR-451a后BxPc3 细胞p-PI3K、p-AKT、TGF-β和p-smad2/3蛋白表达水平明显降低（P<0.05），并且p-PI3K、p-AKT、TGF-β和p-smad2/3蛋白表达水平变化具有浓度依耐性。结论：miR-451a能抑制胰腺癌BxPc3细胞株的增殖、迁移、侵袭能力。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of microRNA (miR)-451a on the proliferation, invasion and migration of pancreatic cancer BxPc3 cells. Methods: BxPc3 cells were cultured in vitro, miR-451a mimics (mimic) were transfected into pancreatic cancer cell line BxPc3 at different concentrations (50, 100, and 200 μmol/L). The expression of miR-451a was detected by fluorescence quantitative polymerase chain reaction (PCR), the MTT proliferation test was used to detect the effect of different concentrations of miR-451a on cell proliferation, and the cell scratch test was used to detect the migration ability of different concentrations of miR-451a of cells. Transwell experiment was used to detect the influence of different concentrations of miR-451a on the invasion ability of cells. Western blot method was used to detect the expression of p-PI3K, p-AKT, TGF-β and p-smad2/3 protein in BxPc3 cells transfected with different concentrations of miR-451a. Results: After transfection with different concentrations of miR-451a mimic, the relative expression of miR-451a in pancreatic cancer BxPc3 cells was significantly increased, and the difference was statistically significant (P<0.05). After overexpression of miR-451a, BxPc3 cell proliferation was significantly weakened, cell migration ability was significantly weakened, cell invasion ability was significantly weakened, and the differences were statistically significant (P<0.05). Compared with the 0 μmol/L group, the expression levels of p-PI3K, p-AKT, TGF-β and p-smad2/3 protein in BxPc3 cells after transfection of 50 and 200 μmol/L miR-451a were significantly reduced (P<0.05), and the change levels of p-PI3K, p-AKT, TGF-β and p-smad2/3 protein are concentration- dependent. Conclusion: miR-451a can inhibit the proliferation, migration and invasion of pancreatic cancer BxPc3 cell line. |
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