文章摘要
金 昊,李福志,张 强,吴 斌,宋晓波.咖啡酸对食管鳞状细胞癌KYSE450裸鼠移植瘤生长的影响及分子机制研究[J].,2022,(6):1033-1037
咖啡酸对食管鳞状细胞癌KYSE450裸鼠移植瘤生长的影响及分子机制研究
The Effects of Caffeic Acid on the Growth of Transplanted Tumors of Esophageal Squamous Cell Carcinoma KYSE450 in Nude Mice and Its Molecular Mechanism
投稿时间:2021-08-23  修订日期:2021-09-18
DOI:10.13241/j.cnki.pmb.2022.06.007
中文关键词: 咖啡酸  食管鳞状细胞癌  裸鼠  移植瘤  细胞凋亡  5-氟尿嘧啶  炎症因子
英文关键词: Caffeic acid  Esophageal squamous cell carcinoma  Nude mice  Transplanted tumor  Apoptosis  5-fluorouracil  Inflammatory factor
基金项目:辽宁省科学技术计划项目(201602208)
作者单位E-mail
金 昊 锦州医科大学第四临床医院(盘锦辽油宝石花医院)心胸外科 辽宁 盘锦 124000 jinhh30018@163.com 
李福志 锦州医科大学附属第三医院胸外科 辽宁 锦州 121000  
张 强 锦州医科大学附属第三医院胸外科 辽宁 锦州 121000  
吴 斌 锦州医科大学附属第三医院胸外科 辽宁 锦州 121000  
宋晓波 锦州医科大学附属第三医院胸外科 辽宁 锦州 121000  
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中文摘要:
      摘要 目的:探讨与研究咖啡酸对食管鳞状细胞癌KYSE450裸鼠移植瘤生长的影响及分子机制。方法:将食管鳞状细胞癌移植瘤裸鼠(n=48)随机平分为三组-模型组、5-氟尿嘧啶组与咖啡酸组。三组分别经腹腔注射0.2 mL生理盐水、5-氟尿嘧啶25 g/kg、5-氟尿嘧啶25 g/kg与咖啡酸50 mg/kg,2次/周,持续4周。结果:5-氟尿嘧啶组与咖啡酸组治疗第2周与第4周的体重高于模型组(P<0.05),咖啡酸组高于5-氟尿嘧啶组(P<0.05)。5-氟尿嘧啶组与咖啡酸组治疗第2周与第4周的肿瘤体积少于模型组(P<0.05),咖啡酸组高于5-氟尿嘧啶组(P<0.05)。5-氟尿嘧啶组与咖啡酸组治疗第4周的血清TNF-α与IL-6含量低于模型组(P<0.05),咖啡酸组低于5-氟尿嘧啶组(P<0.05)。5-氟尿嘧啶组与咖啡酸组治疗第4周的移植瘤Bax、Caspase-3蛋白相对表达水平与凋亡指数高于模型组(P<0.05),咖啡酸组高于5-氟尿嘧啶组(P<0.05)。结论:咖啡酸在食管鳞状细胞癌裸鼠的应用能与5-氟尿嘧啶发挥协同作用,能通过上调Bax、Caspase-3蛋白的表达,促进移植瘤细胞凋亡,抑制炎症因子的表达,减少血管总数,从而抑制移植瘤生长,促进恢复裸鼠体重。
英文摘要:
      ABSTRACT Objective: To explore and study the effects of caffeic acid on the growth of esophageal squamous cell carcinoma KYSE450 transplanted tumors in nude mice and its molecular mechanism. Methods: Nude mice with transplanted esophageal squamous cell carcinoma (n=48) were randomly divided into three groups-model group, 5-fluorouracil group and caffeic acid group. The three groups were injected intraperitoneally with 0.2 mL saline, 5-fluorouracil 25 g/kg, 5-fluorouracil 25g/kg and caffeic acid 50 mg/kg, twice a week for 4 weeks. Results: The body weight of the 5-fluorouracil group and the caffeic acid group were higher than that of the model group in the 2nd and 4th weeks of treatment(P<0.05), and the caffeic acid group were higher than that of the 5-fluorouracil group (P<0.05). The tumor volume in the 5-fluorouracil group and the caffeic acid group at the 2nd and 4th weeks of treatment were less than that of the model group(P<0.05), and the caffeic acid group were higher than that in the 5-fluorouracil group(P<0.05). The levels of serum TNF-α and IL-6 in the 5-fluorouracil group and caffeic acid group were lower than those in the model group(P<0.05), and the caffeic acid group were lower than that in the 5-fluorouracil group(P<0.05) at the 4th weeks of treatment. The relative expression levels of Bax and Caspase-3 protein and apoptosis index of xenograft tumors in the 5-fluorouracil group and caffeic acid group were higher than the model group (P<0.05), and the caffeic acid group were higher than that of the 5-fluorouracil group at the 4th weeks of treatment(P<0.05). Conclusion: The application of caffeic acid in nude mice with esophageal squamous cell carcinoma can play a synergistic effect with 5-fluorouracil. It can promote the apoptosis of transplanted tumor cells, inhibit the expression of inflammatory factors, and reduce the total number of blood vessels by up-regulating the expression of Bax and Caspase-3 proteins. Thereby inhibiting the growth of transplanted tumors, and promoting the restoration of nude mice body weight.
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