| 魏德华,刘珑玲,曾 御,戴依娜,李 慧,陈世玖.淫羊藿苷缓解腹部皮瓣缺血再灌注损伤模型大鼠的作用及机制研究[J].,2022,(6):1024-1027 |
| 淫羊藿苷缓解腹部皮瓣缺血再灌注损伤模型大鼠的作用及机制研究 |
| Effect and Mechanism of Icariin in Alleviating Ischemia-Reperfusion Injury of Abdominal Flap in Rats |
| 投稿时间：2021-07-27 修订日期：2021-08-23 |
| DOI：10.13241/j.cnki.pmb.2022.06.005 |
| 中文关键词: 缺血再灌注损伤 淫羊藿苷 炎症反应 氧化应激 p38 MAPK信号通路 |
| 英文关键词: Ischemia-reperfusion injury Icariin Inflammatory response Oxidative stress P38 MAPK signaling pathway |
| 基金项目:广东省自然科学基金项目（S2012010009371） |
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| 中文摘要: |
| 摘要 目的：研究淫羊藿苷缓解腹部皮瓣缺血再灌注损伤（IRI）模型大鼠的作用及机制。方法：取30只SD级大鼠作为研究对象，将其按照随机数字表法分作假手术组、模型组以及淫羊藿苷组，每组各10只。其中模型组和淫羊藿苷组大鼠均制作大鼠腹部皮瓣IRI模型，假手术组以及模型组大鼠予以生理盐水腹腔注射，淫羊藿苷组大鼠则予以淫羊藿苷腹腔注射。对比各组大鼠皮瓣存活面积及存活率、血清炎症因子以及氧化应激指标水平、皮瓣组织中p38丝裂原活化蛋白激酶（p38 MAPK）信号通路相关蛋白表达情况。结果：模型组、淫羊藿苷组大鼠的皮瓣存活面积及存活率均低于假手术组，但淫羊藿苷组大鼠的皮瓣存活面积及存活率均高于模型组（P＜0.05）。模型组、淫羊藿苷组大鼠血清白细胞介素-10（IL-10）均低于假手术组，但淫羊藿苷组高于模型组；模型组、淫羊藿苷组大鼠血清肿瘤坏死因子-?琢（TNF-?琢）均高于假手术组，但淫羊藿苷组低于模型组（P＜0.05）。模型组、淫羊藿苷组大鼠血清超氧化物歧化酶（SOD）、谷胱甘肽（GSH）水平均低于假手术组，但淫羊藿苷组大鼠血清SOD、GSH水平均高于模型组；模型组、淫羊藿苷组大鼠血清丙二醛（MDA）水平均高于假手术组，但淫羊藿苷组大鼠血清MDA水平低于模型组（P＜0.05）。模型组、淫羊藿苷组大鼠皮瓣组织p38 MAPK、丝裂原活化蛋白激酶磷酸酶-2（MKP-2）相对表达量均高于假手术组，但淫羊藿苷组皮瓣组织p38 MAPK相对表达量低于模型组，而MKP-2相对表达量高于模型组（P＜0.05）。结论：淫羊藿苷可通过调控p38 MAPK信号通路缓解炎症反应及氧化应激，发挥减轻腹部皮瓣IRI的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect and mechanism of icariin in alleviating abdominal flap ischemia-reperfusion injury (IRI) in rats. Methods: A total of 30 SD rats were selected as research subjects, and they were divided into sham operation group, model group and icariin group according to random number table method, with 10 rats in each group. Rat abdominal flap IRI model was made for model group and icariin group, rats in sham operation group and model group were intraperitoneally injected with normal saline, and icariin group was intraperitoneally injected with icariin. The flap survival area and survival rate, the levels of serum inflammatory factors and oxidative stress, and the expression of p38 mitogen activated protein kinase(p38 MAPK) signaling pathway related proteins in the flap tissues were compared in each group of rats. Results: The flap survival area and survival rate in model group and icariin group were lower than those in sham operation group, and the flap survival area and survival rate in icariin group were higher than those in model group (P<0.05). Serum interleukin-10 (IL-10) in model group and icariin group was lower than sham operation group, and icariin group was higher than model group. Serum tumor necrosis factor-α(TNF-α) in model group and icariin group was higher than that in sham operation group, and icariin group was lower than that in model group(P<0.05). The levels of serum superoxide dismutase(SOD), glutathione(GSH) in model group and icariin group were lower than those in sham operation group, and the levels of serum SOD and GSH in icariin group were higher than those in model group. The level of serum malondialdehyde(MDA) in model group and icariin group was higher than that in sham operation group, and the level of serum MDA in icariin group was lower than that in model group(P<0.05). The relative expressions of P38 MAPK and mitogen activated protein kinase Phosphatase-2(MKP-2) in the model group and icariin group were higher than those in the sham operation group, and the relative expressions of P38 MAPK in icariin group was lower than that in the model group, and the relative expressions of MKP-2 was higher than that in the model group(P<0.05). Conclusion: Icariin can alleviate inflammatory response and oxidative stress by regulating p38 MAPK signal pathway, and play a role in reducing IRI of abdominal flap. |
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