| 陈耀华,豆亚伟,王宏涛,田 伟,罗向晖,段宝军.青蒿琥酯对肺癌裸鼠新生血管生成的影响及机制相关研究[J].,2022,(3):442-446 |
| 青蒿琥酯对肺癌裸鼠新生血管生成的影响及机制相关研究 |
| Study on the Effect of Artesunate on Angiogenesis in Nude Mice with Lung Cancer and Its Mechanism |
| 投稿时间：2021-04-07 修订日期：2021-04-30 |
| DOI：10.13241/j.cnki.pmb.2022.03.009 |
| 中文关键词: 青蒿琥酯 肺癌 裸鼠 新生血管生成 结缔组织生长因子 血管内皮生长因子 细胞凋亡 |
| 英文关键词: Artesunate Lung cancer Nude mice Angiogenesis Connective tissue growth factor Vascular endothelial growth factor Apoptosis |
| 基金项目:陕西省科技厅面上项目（2018JM7113） |
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| 中文摘要: |
| 摘要 目的：探讨与研究青蒿琥酯对肺癌裸鼠新生血管生成的影响及机制。方法：27只裸小鼠随机平分为3组-肺癌组、青蒿琥酯1组与青蒿琥酯2组，每组9只。所有小鼠都给予腹腔注射肺癌A549细胞成瘤，致瘤成功后肺癌组、青蒿琥酯1组与青蒿琥酯2组组采用磷酸盐缓冲液、2.0 mg/mL与4.0 mg/mL的青蒿琥酯进行灌胃，1次/d，持续10 d。结果：所有小鼠都致瘤成功，青蒿琥酯1组、青蒿琥酯2组的移植瘤重量低于肺癌组(P<0.05)，抑瘤率高于肺癌组(P<0.05)，青蒿琥酯2组与青蒿琥酯1组对比差异有统计学意义(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤细胞凋亡指数高于肺癌组(P<0.05)，青蒿琥酯2组高于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤组织周围淋巴管密度低于肺癌组(P<0.05)，青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的血清肿瘤坏死因子(Tumor necrosis factor，TNF)-α与白介素(Interleukin，IL)-6水平低于肺癌组(P<0.05)，青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤结缔组织生长因子(Connective tissue growth factor，CTGF)、血管内皮生长因子(Vascular endothelial growth factor，VEGF)蛋白相对表达水平低于肺癌组(P<0.05)，青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。结论：青蒿琥酯在肺癌裸鼠的应用能抑制CTGF、VEGF蛋白表达，降低炎症因子的表达，促进肿瘤细胞凋亡，从而发挥抑制肿瘤增殖与新生血管生成的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and study the effect and mechanism of artesunate on angiogenesis in nude mice with lung cancer. Methods: 27 cases of nude mice were randomly divided into 3 groups-lung cancer group, artesunate 1 group and artesunate 2 groups, with 9 cases in each group. All mice were given intraperitoneal injection of lung cancer A549 cells for tumorigenesis, After successfully tumorigenesis, the lung cancer group, artesunate group 1 and artesunate group 2 were given phosphate buffer, 2.0 mg/mL and 4.0 mg/mL artesunate for intragastric administration, once a day, lasted 10 d. Results: All cases were successfully tumorigenic. The weight of transplanted tumors in artesunate 1 group and artesunate 2 group were lower than that of the lung cancer group (P<0.05), and the tumor inhibition rate were higher than that of the lung cancer group(P<0.05), there were statistically significant difference compared between artesunate group 2 and artesunate group 1(P<0.05). The apoptosis index of transplanted tumor cells in artesunate group 2 and artesunate group 1 were higher than that of lung cancer group(P<0.05), and artesunate group 2 were higher than artesunate group 1(P<0.05). The density of lymphatic vessels around transplanted tumor tissues in artesunate 2 group and artesunate 1 group were lower than that of lung cancer group(P<0.05), and artesunate 2 group were lower than artesunate 1 group(P<0.05). The levels of serum tumor necrosis factor (TNF)-α and interleukin(IL)-6 in artesunate group 2 and artesunate group 1 were lower than those in lung cancer group(P<0.05), the artesunate group 2 were lower than the artesunate 1 group(P<0.05). The relative expression levels of connective tissue growth factor (CTGF) and vascular endothelial growth factor(VEGF) proteins in transplanted tumors in artesunate group 2 and artesunate group 1 were lower than those in the lung cancer group(P<0.05), the artesunate group 2 were lower than the artesunate group 1 (P<0.05). Conclusion: The application of artesunate in nude mice with lung cancer can inhibit the expression of CTGF and VEGF protein, reduce the expression of inflammatory factors, and promote tumor cell apoptosis, thereby inhibit tumor proliferation and angiogenesis. |
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