| 巩 亮,王晓春,焦 阳,姜顺涛,杨光磊.盐敏感性高血压大鼠纹蛋白水平变化对醛固酮及p38MAPK通路的影响[J].,2022,(3):437-441 |
| 盐敏感性高血压大鼠纹蛋白水平变化对醛固酮及p38MAPK通路的影响 |
| Effect of Stria Protein Level on Aldosterone and p38MAPK Pathway in Salt-sensitive Hypertensive Rats |
| 投稿时间：2021-04-07 修订日期：2021-04-30 |
| DOI：10.13241/j.cnki.pmb.2022.03.008 |
| 中文关键词: 盐敏感性高血压 醛固酮 依普利酮 striatin p38 MAPK |
| 英文关键词: Salt sensitive hypertension Aldosterone Eplerenone Striatin p38 MAPK |
| 基金项目:贵州省科技厅项目（黔科合基础[2016]1173） |
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| 中文摘要: |
| 摘要 目的：探讨盐敏感性高血压大鼠纹蛋白水平变化对醛固酮及p38MAPK通路的影响。方法：通过向新生大鼠皮下注射辣椒素和高盐饮食构建盐敏感性高血压大鼠模型，分别使用醛固酮（ALDO）和醛固酮、依普利酮联合给药，实验分组为：1）正常对照组；2）模型组；3）假手术组；4）ALDO组；5）ALDO+依普利酮组。通过ELISA检测大鼠血浆中ALDO含量和外周血肾素含量，通过qPCR和WB检测大鼠心脏、肾脏、血管平滑肌中striatin和p38 MAPK的mRNA和蛋白的表达变化。结果：造模后在给药前大鼠血压有不同程度的升高，给药后ALDO组和ALDO+ Eplerenone组血压均有一定程度的下降；给药后ALDO+Eplerenone组大鼠血浆中ALDO和外周血中肾素含量升高；在肾脏与Control组和Model组相比，ALDO+Eplerenone组的striatin和p38 MAPK蛋白表达水平显著升高，p38 MAPK的 mRNA表达水平显著升高；在心脏中与Model组相比，ALDO+Eplerenone组的p38 MAPK蛋白表达水平显著升高；在主动脉中与Control组和Model组相比，ALDO+Eplerenone组的striatin和p38 MAPK蛋白表达水平显著降低。结论：纹蛋白水平的改变与盐敏感高血压具有相关性，其可通过调节ALDO/MR→p38MAPK相关通路影响盐敏感性高血压。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of changes in the level of Striarin on aldosterone and p38MAPK pathways in salt-sensitive hypertensive rats. Methods: A salt-sensitive hypertensive rat model was constructed by subcutaneously injecting capsaicin and a high-salt diet into neonatal rats. Aldosterone (ALDO) was administered in combination with aldosterone and eplerenone. The experiment was divided into: 1) Normal control Group; 2) Model group; 3) Sham operation group; 4) ALDO group; 5) ALDO+eplerenone group. The content of ALDO in rat plasma and the content of renin in peripheral blood were detected by ELISA, and the mRNA and protein expression of striatin and p38 MAPK in rat heart, kidney, and vascular smooth muscle were detected by qPCR and WB. Results: After the model was established, the blood pressure of the rats increased to varying degrees before the administration. After the administration, the blood pressure of the ALDO group and the ALDO+Eplerenone group both decreased to a certain extent; after the administration, the ALDO+Eplerenone group rat plasma ALDO and peripheral The blood renin content increased; compared with the Control group and the Model group in the kidney, the striatin and p38 MAPK protein expression levels in the ALDO+Eplerenone group were significantly increased, and the p38 MAPK mRNA expression level was significantly increased; in the heart, compared with the Model group Compared with the ALDO+Eplerenone group, the expression level of p38 MAPK protein was significantly increased; in the aorta, compared with the Control group and the Model group, the expression levels of striatin and p38 MAPK protein in the ALDO+Eplerenone group were significantly reduced. Conclusion: The change of stroma protein level is related to salt-sensitive hypertension, which can affect salt-sensitive hypertension by regulating ALDO/MR→p38MAPK related pathways. |
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