| 刘 虹,王洁英,刘 娜,韩田田,白涛敏.布地奈德混悬液对哮喘模型小鼠肺组织TLR4/MyD88/NF-κB通路的影响[J].,2021,(23):4432-4435 |
| 布地奈德混悬液对哮喘模型小鼠肺组织TLR4/MyD88/NF-κB通路的影响 |
| Effect of Budesonide Suspension on TLR4 / MyD88 / NF-κB Pathway in Lung Tissue of Asthmatic Mice |
| 投稿时间：2021-04-06 修订日期：2021-04-30 |
| DOI：10.13241/j.cnki.pmb.2021.23.007 |
| 中文关键词: 布地奈德混悬液 哮喘 肺组织 TLR4 MyD88 NF-κB |
| 英文关键词: Budesonide suspension Asthma Lung tissue TLR4 MyD88 NF-κB |
| 基金项目:陕西省国际间合作项目—— 一般项目（2018KW-041） |
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| 中文摘要: |
| 摘要 目的：探究布地奈德混悬液对哮喘模型小鼠肺组织TOLL样受体4（toll-like receptor 4，TLR4）/髓样分化因子88（myeloid differentiation factor 88，MyD88）/核因子κB（nuclear factor-κB，NF-κB）通路的影响。方法：使用4 %鸡蛋清白蛋白与2 %的Al（OH3）共同致敏小鼠，建立咳嗽变异性哮喘小鼠模型40只，将模型大鼠分别使用低、中、高剂量（0.2、1.0、2.0 g/kg）布地奈德混悬液和孟鲁司特钠进行干预，1次/日连续干预14 d，于干预14 d时采集小鼠的支气管肺泡灌注液（bronchoalveolar lavage fluid，BALF）、气管及肺组织，对各组BALF中的白细胞（white blood cell，WBC）、嗜酸性粒细胞、血清γ干扰素（interferon-γ，IFN-γ）、白细胞介素-1β（interleukin-1β，Il-1β）、肿瘤坏死因子-α(tumor necrosis factor-α，TNF-α)水平差异开展比较，对各组小鼠肺组织黏膜上皮增生程度评分、炎症细胞浸润程度评分、病变总评分差异，以及TLR4、MyD88、p65蛋白表达差异进行分析。结果：分析显示，布地奈德混悬液能够显著降低哮喘模型小鼠BALF中白细胞及嗜酸性粒细胞数量，同时还能够改善小鼠气管和支气管黏膜上皮增生与肺组织炎症细胞浸润状态，且干预后小鼠肺组织中的TLR4、MyD88、p65蛋白表达水平出现了明显的降低。结论：布地奈德混悬液对改善小鼠哮喘效果较好，其作用机制可能与该药能够调节TLR4、MyD88、p65蛋白表达，进而影响炎症和免疫反应进程有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of budesonide suspension on Toll like receptor 4 (TLR4) / myeloid differentiation factor 88 (MyD88) / nuclear factor κB in lung tissue of asthmatic mice. Methods: 40 cough variant asthma mice models were established by CO sensitizing mice with 4 % ovalbumin and 2 % Al (OH3). The model rats were intervened with low, medium and high doses (0.2, 1.0 and 2.0 g/kg) of budesonide suspension and montelukast sodium respectively, once a day for 14 days, and the bronchoalveolar tissues were collected at the time of intervention for 14 days The levels of leukocytes (WBC), eosinophils, IFN-γ, IL-1β and TNF-α in BALF of each group were compared, and the differences in the scores of epithelial hyperplasia, inflammatory cell infiltration and total pathological changes, as well as the expression of TLR4, MyD88 and p65 proteins in lung tissue of each group were analyzed. Results: Budesonide suspension can significantly reduce the number of leukocytes and eosinophils in BALF of asthmatic model mice, and can also improve the airway and bronchial epithelial hyperplasia and inflammatory cell infiltration in lung tissue of mice, and the expression levels of TLR4, MyD88 and p65 protein in lung tissue of mice decreased significantly after intervention. Conclusion: Budesonide suspension can improve the asthma model mice The mechanism of action may be related to the regulation of TLR4, MyD88, p65 protein expression, and then affect the process of inflammation and immune response. |
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