| 莫娟梅,甘芷川,张顺荣,郑 盼,张洪瑞,梁新梅,农复香,林 威.扶正方对Lewis肺癌小鼠免疫功能、PI3K/AKT信号通路和外周血IL-2、IL-6、INF-γ的影响[J].,2021,(23):4422-4426 |
| 扶正方对Lewis肺癌小鼠免疫功能、PI3K/AKT信号通路和外周血IL-2、IL-6、INF-γ的影响 |
| Effects of Fuzhengfang on Immune Function, PI3K/AKT Signaling Pathway and IL-2, IL-6, INF-γ in Peripheral Blood of Lewis Lung Cancer Mice |
| 投稿时间：2021-03-22 修订日期：2021-04-17 |
| DOI：10.13241/j.cnki.pmb.2021.23.005 |
| 中文关键词: 扶正方 Lewis肺癌小鼠 PI3K/AKT信号通路 免疫功能 细胞因子 |
| 英文关键词: Fuzhengfang Lewis lung cancer mice PI3K/AKTsignaling pathway Immune function Cytokines |
| 基金项目:广西壮族自治区中医药管理局自筹经费科研项目（GZZC2020133）；广西中医药大学2019～2021年广西一流学科建设开放课题（2019XK065）；广西国际壮医医院院级课题（2019005） |
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| 中文摘要: |
| 摘要 目的：观察扶正方对Lewis肺癌小鼠免疫功能、磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（AKT）信号通路和外周血白细胞介素（IL）-2、IL-6、γ干扰素（INF-γ）的影响。方法：将40只Lewis肺癌小鼠随机分为模型组（M组）、扶正方低剂量组（A组）、扶正方高剂量组（B组）、顺铂组（S组），每组10只，A组、B组分别给予扶正方0.4 mL/20 g、0.8 mL/20 g灌胃，M组给予生理盐水0.4 mL/20 g灌胃，S组给予顺铂1 mg/mL，0.4 mL灌胃，连续14d，比较各组小鼠一般情况、肿瘤重量，胸腺指数、脾脏指数、脾脏CD3+细胞、CD4+细胞、CD8+细胞比例细胞百分比，鼠肿瘤组织PI3K/AKT信号通路蛋白表达水平及外周血IL-2、IL-6、INF-γ水平。结果：A组、B组、S组小鼠肿瘤重量低于M组，S组小鼠肿瘤重量低于A组、B组（P<0.05），治疗前各组小鼠体重比较无统计学差异（P>0.05），治疗后A组、B组小鼠体重高于S组、M组（P<0.05）。A组、B组小鼠胸腺指数显著高于M组、S组（P<0.05）。A组、B组CD3+、CD4+、CD4+/CD8+显著高于M组、S组，CD8+显著低于M组、S组（P<0.05），B组CD3+、CD4+、CD4+/CD8+显著高于A组，CD8+低于A组（P<0.05）。A组、B组、S组小鼠肿瘤组织PI3K蛋白、AKT蛋白表达水平显著低于M组（P<0.05）。A组、B组、S组小鼠外周血IL-2、INF-γ水平显著高于M组，IL-6水平显著低于M组（P<0.05）。结论：扶正方可以提升Lewis肺癌小鼠免疫功能，调节IL-2、IL-6、INF-γ细胞因子水平，抑制PI3K/AKT信号通路起到抗肺癌的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effects of Fuzhengfang on immune function, phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway and interleukin (IL)-2, IL-6, interferonγ(INF-γ) in peripheral blood of Lewis lung cancer mice. Methods: 40 Lewis lung cancer mice were randomly divided into model group (group M), fuzhengfang low dose group (group A), fuzhengfang high dose group (group B) and cisplatin group (group S), 10 mice in each group. Group A and group B were given fuzhengfang 0.4 mL/20 g and 0.8 mL/20 g by intragastric gavage, respectively, group M was given normal saline 0.4 mL/20 g by intragastric gavage, and group S was given cisplatin 1 mg/mL and 0.4 mL by intragastric gavage, continuous 14d. The general condition, tumor weight, thymus index, spleen index, the proportion of CD3+ cells, CD4+ cells and CD8+ cells in spleen, the protein expression level of PI3K/ AKT signaling pathway in tumor tissues and the levels of IL-2, IL-6 and INF-γ in peripheral blood of mice were compared. Results: The tumor weight of mice in group A, group B and group S were lower than that in group M, the tumor weight of mice in group S was lower than that in group A and group B (P<0.05). There was no significant difference in the weight of mice in each group before treatment (P>0.05). After treatment, the weight of mice in group A and group B were higher than that in group S and group M (P<0.05). The thymus index in group A and group B were significantly higher than that of group M and group S (P<0.05). CD3+, CD4+, CD4+/CD8+ in group A and group B were significantly higher than those in group M and group S, CD8+ was significantly lower than those in group M and group S (P<0.05), and CD3+, CD4+, CD4+/CD8+ in group B were significantly higher than those in group A, CD8+ was lower than that in group A (P<0.05). The PI3K protein and AKT protein expression levels in tumor tissues of group A, group B and group S were significantly lower than those of group M (P<0.05). IL-2 and INF- γ in peripheral blood of mice in group A, group B and group S were significantly higher than those in group M, and the IL-6 level was significantly lower than that in group M (P<0.05). Conclusion: Fuzhengfang can enhance the immune function of Lewis lung cancer mice, and regulate the levels of IL-2, IL-6 and INF-γ cytokines, inhibit PI3K/ AKT signaling pathway and play an anti-lung cancer role. |
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