| 胡鹏刚,田克勇,毛小波,王人凤,查定军.顺铂耳毒性大鼠耳聋模型的研究[J].,2021,(23):4412-4416 |
| 顺铂耳毒性大鼠耳聋模型的研究 |
| A Study on Cisplatin-induced Hearing Loss in a Rat Model |
| 投稿时间：2021-01-28 修订日期：2021-02-25 |
| DOI：10.13241/j.cnki.pmb.2021.23.003 |
| 中文关键词: 顺铂 感音神经性聋 毛细胞 螺旋神经元 |
| 英文关键词: Cisplatin Sensorineural hearing loss Hair cell Spiral ganglion neuron |
| 基金项目:国家自然科学基金项目（81870732）； 海南省自然科学基金项目（817407）；陕西省科技重点项目（2018PT-01）；陕西省科技重点项目（2017ZDXM-SF-061）； 西京学科助推科研项目（XJZT19MJ05） |
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| 中文摘要: |
| 摘要 目的：探讨顺铂对大鼠造成的听力损伤及耳蜗细胞形态学变化。方法：体内实验，运用顺铂腹腔注射的方法，连续七天注射，通过听性脑干反应检测，观察顺铂对不同日龄的大鼠听力损伤情况；测听后取耳蜗，通过基底膜铺片和冰冻切片的免疫荧光染色，观察听力损伤后对耳蜗毛细胞和螺旋神经元的影响。体外实验，耳蜗器官培养免疫荧光染色，观察顺铂对耳蜗毛细胞和螺旋神经元的影响。结果：顺铂具有耳毒性，会对大鼠听力造成损伤，高频听力损伤更加严重，而且对不同日龄的大鼠造成的听力损失不同，小日龄的大鼠对顺铂耳毒性更加敏感。体内实验，顺铂耳毒性造成听力损失，会引起大鼠耳蜗毛细胞的缺失，但未观察到明显的螺旋神经元缺失，也没有观察到明显的Cleaved caspase-3阳性螺旋神经元细胞。体外实验，可以观察到顺铂同时引起毛细胞和螺旋神经元产生明显的损伤。结论：体、内外实验，都可以建立稳定的顺铂耳毒性大鼠耳聋模型，对研究顺铂损伤耳蜗毛细胞的发生机制和保护奠定了实验基础。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the hearing loss and morphological changes caused by cisplatin in rats. Methods: In vivo, cisplatin was injected intraperitoneally for seven consecutive days to observe the hearing loss of different age through the detection of auditory brainstem reaction. Cochleae were taken after hearing measurement, and the morphological changes of the cochlear hair cells and spiral ganglion neurons were observed by immunofluorescence staining of the basilar membrane and cochlear frozen section. In vitro, the effects of cisplatin on hair cells and spiral ganglion neurons were observed by immunofluorescence staining of cochlear organotypic cultures. Results: Cisplatin had ototoxicity and could cause hearing loss in rats. The damage of high-frequency hearing performance was more serious. Moreover, the hearing loss was different in the rats of different postnatal days, and the rats of younger days were more sensitive to cisplatin ototoxicity. In vivo, hearing loss induced by cisplatin resulted in loss of cochlear hair cells in rats, but no significant loss of spiral ganglion neurons was observed, nor were Cleaved caspase-3 positive spiral ganglion neurons found. In vitro, cisplatin ototoxicity caused significant damage to both hair cells and spiral ganglion neurons. Conclusion: Both in vivo and in vitro experiments can establish a stable cisplatin-induced hearing loss model in rats, which will lay a foundation for the study on the mechanism and protection of cisplatin induced cochlear hair cells. |
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