| 王雪慧,伍思琪,李 孟,刘紫君,高风丽,李 星.平喘颗粒对慢性哮喘大鼠Notch通路相关因子的影响[J].,2021,(23):4407-4411 |
| 平喘颗粒对慢性哮喘大鼠Notch通路相关因子的影响 |
| Effects of Pingchuan Granules on Notch Pathway Related Factors in Chronic Asthmatic Rats |
| 投稿时间：2020-12-29 修订日期：2021-01-25 |
| DOI：10.13241/j.cnki.pmb.2021.23.002 |
| 中文关键词: 平喘颗粒 支气管哮喘 气道重塑 Notch2 Jagged1 |
| 英文关键词: Pingchuan Granules Bronchial asthma Airway remodeling Notch2 Jagged1 |
| 基金项目:国家自然科学基金面上项目（82074365）；博士后研究人员落户黑龙江科研启动资助基金项目（LBH-Q17172）； 黑龙江中医药大学"双一流"中西医结合学科优秀青年基金面上项目（051022）； 中华中医药学会青年人才托举工程项目（CACM-2018-QNRC2-C15）； 黑龙江中医药大学科研基金项目杰出培育基金（2019JC04）； 黑龙江省第一批省级名中医师承工作；黑龙江中医药大学2019年度校科研基金面上项目（2019MS04） |
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| 中文摘要: |
| 摘要 目的：通过观测各组慢性哮喘大鼠Notch信号通路相关因子Notch2、Jagged1、HES-1的变化，以及肺组织气道重塑的改善状况，探讨平喘颗粒在哮喘的治疗方面所发挥的作用机制。方法：按随机法将60只大鼠均分至空白组、模型组、地塞米松组、平喘颗粒组中。除空白组外，剩余的3组大鼠经卵蛋白+氢氧化铝致敏，复制慢性哮喘大鼠模型，空白组用生理盐水代替致敏液。造模前各组予相应的药物灌胃处理。6周后造模完成，处死大鼠并取其肺组织进行HE染色，在光镜下观察病理变化。使用Real-time PCR法检测Notch2、Jagged1 mRNA，Western blot法检测HES-1蛋白的表达程度。结果：与空白组相比，其余各组肺组织均可观察到明显气道重塑改变，Notch2、Jagged1 mRNA及HES-1蛋白表达明显上升（P＜0.05）；与模型组相比，平喘颗粒和地塞米松可缓解气道重塑，各因子表达情况显著下降（P＜0.05），两组间比较则无明显差异（P＞0.05）。结论：Notch2、Jagged1、HES-1表达程度与气道重塑呈正相关，平喘颗粒通过降低Notch2、Jagged1、HES-1表达改善气道重塑，可能是其防治哮喘发生发展的机制。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of Pingchuan Granules in the treatment of asthma by observing the changes of Notch2, Jagged1, HES-1 related factors of Notch signaling pathway in chronic asthma rats in each group, as well as the improvement of lung tissue airway remodeling. Methods: On the basis of random number table method, 60 rats were divided into normal group, model group, dexamethasone group and Pingchuan granules group on average. Except the normal group, the rest of 3 groups were sensitized by intraperitoneal injection of ovalbumin (OVA) and Al(OH)3, and the asthmatic rat model was duplicated. The normal group was treated with normal saline instead of sensitizing solution. Before modeling, each group was given the corresponding drug gavage treatment. Six weeks later, the rats were killed and their lung tissues were stained with HE. The pathological changes were observed under light microscope. Real-time PCR was used to detect Notch2 and Jagged1 mRNA, and Western blot was used to detect HES-1 protein. Results: Compared with the normal group, significant changes in airway remodeling were observed in the lung tissues of the other groups, and the expressions of Notch2, Jagged1 mRNA and HES-1 protein were significantly increased (P<0.05). Compared with the model group, pingchuan granules and dexamethasone could alleviate airway remodeling, and the expression of each factor was significantly decreased (P<0.05), but there was no significant difference between the two groups (P>0.05). Conclusion: The expression levels of Notch2, Jagged1 and HES-1 are positively correlated with airway remodeling, and the improvement of airway remodeling by reducing the expression levels of Notch2, Jagged1 and HES-1 may be the mechanism for the prevention and treatment of asthma. |
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