| 程丽宪,杨蝉联,林纳荣,祝姗姗,刘煌辉,林泓羽,陈晓瑜,江佩颖,张 敏,江天琪.脂联素抑制子宫内膜癌HEC-1B细胞增殖、迁移及侵袭的作用及机制研究[J].,2021,(20):3814-3817 |
| 脂联素抑制子宫内膜癌HEC-1B细胞增殖、迁移及侵袭的作用及机制研究 |
| Study on the Effect and Mechanism of Adiponectin on the Proliferation, Migration and Invasion of HEC-1B Cells in Endometrial Carcinoma |
| 投稿时间：2021-03-11 修订日期：2021-04-08 |
| DOI：10.13241/j.cnki.pmb.2021.20.003 |
| 中文关键词: 脂联素 子宫内膜癌 增殖 迁移 侵袭 腺苷酸活化蛋白激酶信号通路 |
| 英文关键词: Adiponectin Endometrial carcinoma Proliferation Migration Invasion Adenosine monophosphate activated protein kinase |
| 基金项目:福建省卫生计生委青年科研项目（2017-2-116）；福建省中青年教师教育科研项目（科技类）（JT180652）；2018年福建省高校杰出青年科研人才培养计划（闽教科（2018）47号） |
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| 中文摘要: |
| 摘要 目的：探讨脂联素（APN）对子宫内膜癌HEC-1B细胞增殖、迁移及侵袭的抑制作用及分子机制。方法：分别采用磺酰罗丹明 B(SRB)实验、细胞迁移(Transwell)实验和划痕实验检测子宫内膜癌细胞HEC-1B的增殖、迁移和侵袭能力。采用蛋白免疫印迹（Western blot）法检测腺苷酸活化蛋白激酶(AMPK)信号通路相关蛋白、AdipoR1、AdipoR2、cyclinD1和cyclinE2蛋白表达水平。结果：与对照组相比，APN组HEC-1B细胞增殖、迁移及侵袭功能明显下降（P<0.05）。与对照组相比，APN组p-AMPK/AMPK比值明显提高，而p-mTOR/mTOR和p-4EBP1/4EBP1比值明显下降（P<0.05）。与对照组相比，APN组cyclinD1和cyclinE2蛋白表达水平明显下降（P<0.05）。APN组和对照组的AdipoR1、AdipoR2蛋白表达水平比较无统计学差异（P>0.05）。结论：APN能够激活AMPK信号通路并下调cyclinD1和cyclinE2蛋白表达，进而抑制子宫内膜癌细胞的增殖、迁移和侵袭功能。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the inhibitory effect of adiponectin (APN) on the proliferation, migration and invasion of endometrial carcinoma HEC-1B cells and its molecular mechanism. Methods: The proliferation, migration and invasion of endometrial cancer cells HEC-1B were detected by sulfonyl rhodamine B(SRB) experiment and the cell migration (Transwell) experiment and scratch tests, respectively. Western blot was used to detect the expression levels of adenosine monophosphate activated protein kinase (AMPK) signaling pathway related proteins, AdipoR1, AdipoR2, cyclinD1 and cyclinE2. Results: Compared with the control group, the proliferation, migration and invasion of HEC-1B cells in APN group were significantly decreased (P<0.05). Compared with the control group, the p-AMPK/AMPK ratio in the APN group was significantly increased, while the p-MTOR/mTOR and p-4EBp1 /4EBP1 ratios were significantly decreased(P<0.05). Compared with the control group, the protein expression levels of cyclinD1 and cyclinE2 in the APN group were significantly decreased(P<0.05). There was no significant difference in the expression of AdipoR1 and AdipoR2 between APN group and control group(P>0.05). Conclusion: APN can activate AMPK signaling pathway and down-regulate the expression of cyclinD1 and cyclinE2 proteins, thereby inhibiting the proliferation, migration and invasion of endometrial cancer cells. |
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