| 刘旭剑,王东来,李增怀,冯 奇,常富军,冯建刚.miR-29a对于膝关节骨性关节炎大鼠滑膜损伤中的保护作用研究[J].,2021,(19):3649-3653 |
| miR-29a对于膝关节骨性关节炎大鼠滑膜损伤中的保护作用研究 |
| Protective Effect of miR-29a on Synovial Injury in Rats with Knee Osteoarthritis |
| 投稿时间：2021-04-23 修订日期：2021-05-18 |
| DOI：10.13241/j.cnki.pmb.2021.19.010 |
| 中文关键词: miR-29a 膝关节骨性关节炎 滑膜损伤 机制 |
| 英文关键词: miR-29a Knee osteoarthritis Synovial injury Mechanism |
| 基金项目:河北省医学科学研究重点课题计划项目（20170759） |
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| 中文摘要: |
| 摘要 目的：探讨miR-29a对于膝关节骨性关节炎（KOA）大鼠滑膜损伤中的保护作用研究。方法：采用前交叉韧带横断法（ACLT）建立KOA大鼠模型。大鼠注射microRNA阴性对照和miR-29a。通过实时定量聚合酶链反应（RT-qPCR）检测KOA滑膜组织和滑膜细胞中miR-29a的表达。RT-qPCR和蛋白免疫印迹试验检测Toll样受体4/髓样分化蛋白88/核因子κB（TLR4/Myd88/NF-κB）信号通路相关蛋白的表达。检测KOA滑膜组织及滑膜细胞中炎症因子的表达水平。结果：KOA滑膜组织和滑膜细胞中miR-29a表达下调。上调miR-29a可抑制KOA大鼠滑膜细胞的炎症反应，促使KOA大鼠的TLR4/Myd88/NF-κB信号通路失活。结论：上调miR-29a可通过TLR4/Myd88/NF-κB信号通路失活化抑制KOA大鼠滑膜细胞炎症反应，从而保护滑膜损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the protective effect of miR-29a on synovial injury in rats with knee osteoarthritis (KOA). Methods: A KOA rat model was established by anterior cruciate ligament transection (ACLT). Rats were injected with microRNA- negative control and miR-29a. miR-29a expression in synovial tissue and synovial cells of KOA was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Toll-like receptor 4/ myeloid differentiation protein 88/ nuclear factor κB (TLR4/MyD88/NF-κB) signaling pathway related protein expression was detected by RT-qPCR and Western blot assay. The expression levels of inflammatory cytokines in KOA synovial tissue and synovial cells were detected. Results: miR-29a expression was down-regulated in KOA synovial tissue and synovial cells. Up-regulation of Mir-29a inhibits the inflammatory response of synoviocytes in KOA rats, and inactivates the TLR4/Myd88/NF-κB signaling pathway in KOA rats. Conclusion: The up-regulation of miR-29a inhibited the inflammatory response of synovial cells in KOA rats through the inactivation of TLR4/MyD88/NF-κB signaling pathway, thereby protecting the synovial injury. |
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