| 蔡 斌,马 洁,李林霞,桑震宇,陈华莉.千金藤素腹腔注射对卵巢癌模型大鼠的作用及其机制研究[J].,2021,(19):3607-3611 |
| 千金藤素腹腔注射对卵巢癌模型大鼠的作用及其机制研究 |
| Study on the Effect and Mechanism of Qianjin Tensu Intraperitoneal Injection on Ovarian Cancer Model Rats |
| 投稿时间：2021-05-06 修订日期：2021-05-31 |
| DOI：10.13241/j.cnki.pmb.2021.19.002 |
| 中文关键词: 卵巢癌 千金藤素 作用机制 PI3K/mTOR信号通路 |
| 英文关键词: Ovarian cancer Qianjin tensu Effect and mechanism PI3K/mTOR signaling pathway |
| 基金项目:上海市自然科学基金面上项目（12ZR1424200） |
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| 中文摘要: |
| 摘要 目的：研究千金藤素腹腔注射对卵巢癌模型大鼠的作用及其机制。方法：选取30只SD级清洁级健康雌性大鼠，按照随机数字表法分成模型组、实验组与空白对照组，每组各10只。空白对照组不予以处理，模型组与实验组建立卵巢癌模型，且实验组在造模后予以千金藤素腹腔注射干预，使用剂量为20 mg/kg每次，1次/d，给药30d，模型组与空白对照组予以等量生理盐水干预。比较各组大鼠荷瘤重量、腹水量以及肿瘤体积，癌胚抗原（CEA）、糖类抗原153（CA153）及人附睾蛋白（HE4）表达水平，磷脂酰肌醇3-激酶（PI3K）、雷帕霉素靶蛋白（p-mTOR）、张力蛋白同源缺失的染色体（PTEN）表达，免疫指标水平。结果：模型组与实验组大鼠荷瘤重量、腹水量、肿瘤体积及CEA、CA153、HE4表达水平均高于空白对照组，且实验组大鼠上述各项指标水平均低于模型组（均P＜0.05）。模型组及实验组大鼠的PI3K、p-mTOR、PTEN表达水平均低于空白对照组，且实验组大鼠的PI3K、p-mTOR、PTEN表达水平均高于模型组（均P＜0.05）。模型组及实验组大鼠的CD4+、CD4+/CD8+水平均低于空白对照组，且实验组大鼠CD4+、CD4+/CD8+水平均高于模型组（均P＜0.05）；模型组及实验组大鼠的CD8+水平均高于空白对照组，且实验组大鼠CD8+水平低于模型组（均P＜0.05）。结论：千金藤素腹腔注射对卵巢癌模型大鼠具有明显的抑癌作用，有利于下调肿瘤标志物水平，其主要作用机制可能与调控PI3K/mTOR信号通路、PTEN表达以及免疫功能有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect and mechanism of qianjin tensu intraperitoneal injection on ovarian cancer model rats. Methods: 30 SD clean grade healthy female rats were selected, and divided into model group, experimental group and blank control group according to random number table method, 10 rats in each group. No treatment was given to the blank control group. Ovarian cancer model was established between the model group and the experimental group. After modeling, qianjin tensu intraperitoneal injection was given to the experimental group at a dose of 20 mg/kg each time, 1 times/d, and administered for 30d. The model group and the blank control group were given the same amount of normal saline intervention. The tumor-bearing weight, abdominal water volume, tumor volume, carcinoembryonic antigen (CEA), carbohydrate antigen 153 (CA153), human epididymal protein (HE4) expression, phosphatidylinositol 3-kinase (PI3K), rapamycin target protein (p-mTOR), homologous deletion of tensin chromosome (PTEN) expression and immune indicators of each group were compared. Results: The tumor-bearing weight, abdominal water volume, tumor volume and CEA, CA153, HE4 of the rats in the model group and the experimental group were all higher than those in the blank control group, and the levels of the above indicators in the experimental group were lower than those in the model group (all P＜0.05). The expressions levels of PI3K, p-mTOR and PTEN in the model group and the experimental group were all lower than those in the blank control group, and the expressions levels of PI3K, P-MTOR and PTEN in the experimental group were all higher than those in the model group (all P＜0.05). The levels of CD4+ and CD4+/CD8+ in the model group and the experimental group were lower than those in the blank control group, and the levels of CD4+ and CD4+/CD8+ in the experimental group were higher than those in the model group (all P＜0.05). The level of CD8+ in the model group and the experimental group were higher than that in the blank control group, and the level of CD8+ in the experimental group was lower than that in the model group (all P＜0.05). Conclusion: Qianjin tensu intraperitoneal injection has an obvious anticancer effect on ovarian cancer model rats, which is beneficial to down-regulation of tumor markers, and its main mechanism may be related to the regulation of PI3K/mTOR signaling pathway, PTEN expression and immune function. |
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