文章摘要
陈艳霞,哈尼克孜·吐尔逊,梁凌云,刘新莲,马彩玲.长链非编码RNA SNHG14/miR-211对宫颈癌细胞的增殖、侵袭能力的影响[J].,2021,(14):2622-2625
长链非编码RNA SNHG14/miR-211对宫颈癌细胞的增殖、侵袭能力的影响
Effects of Long Non-coding RNA SNHG14/miR-211 on the Proliferation and Invasion of Cervical Cancer Cells
投稿时间:2021-02-01  修订日期:2021-02-23
DOI:10.13241/j.cnki.pmb.2021.14.005
中文关键词: miR-211  长链非编码RNA SNHG14  宫颈癌  细胞增殖  细胞侵袭  黏蛋白4
英文关键词: MiR-211  Long non-coding RNA SNHG14  Cervical cancer  Cell proliferation  Cell invasion  Mucin 4
基金项目:新疆维吾尔自治区自然科学基金面上项目(2020D01C238);省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2020-16;SKL-HIDCA-2019-5)
作者单位E-mail
陈艳霞 新疆医科大学第一附属医院妇科 新疆 乌鲁木齐830054 chenyanxia197907@163.com 
哈尼克孜·吐尔逊 新疆医科大学第一附属医院妇科 新疆 乌鲁木齐830054  
梁凌云 新疆医科大学第一附属医院妇科 新疆 乌鲁木齐830054  
刘新莲 新疆医科大学第一附属医院妇科 新疆 乌鲁木齐830054  
马彩玲 新疆医科大学第一附属医院妇科 新疆 乌鲁木齐830054  
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中文摘要:
      摘要 目的:探讨长链非编码(Long chain non-coding,Lnc)RNA SNHG14/微小RNA(microRNA,miR)-211对宫颈癌细胞的增殖、侵袭能力的影响。方法:宫颈癌细胞株SiHa设三组:空白组(不进行转染)、对照组(转染miR-NC)与miR-211组(转染miR-211 mimic), 噻唑蓝[3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide,MTT] 检测细胞增殖活性,Transwell检测细胞侵袭及转移,实时荧光定量核酸扩增检测系统(Real-time Quantitative PCR, qPCR)检测LncRNA SNHG14与miR-211mRNA水平,Westem印迹法检测黏蛋白4(mucin 4, MUC4)蛋白水平。结果:转染后24 h与48 h,miR-211组的细胞增殖、侵袭、转移指数与LncRNA SNHG14与MUC4蛋白相对表达水平低于空白组和对照组(P<0.05),miR-211 mRNA表达水平高于空白组(P<0.05),空白组与对照组对比差异无统计学意义(P>0.05)。结论:过表达miR-211可抑制LncRNA SNHG14的表达,也能抑制MUC4表达,从而能抑制宫颈癌细胞的增殖、侵袭及转移。
英文摘要:
      ABSTRACT Objective: To investigate the effects of long chain non-coding (Lnc) RNA SNHG14/microRNA(miR)-211 on the proliferation and invasion of cervical cancer cells. Methods: Cervical cancer cell line SiHa were divided into three groups: blank group (without transfection), control group (transfected with miR-NC) and miR-211 group (transfected with miR-211 mimic), used the MTT to detect cell proliferation activity, Transwell to detect the cell invasion and metastasis, qPCR to detect of LncRNA SNHG14 and miR-211 mRNA levels, Western blotting to detect of of Mucin 4(MUC4) protein levels. Results: At 24 h and 48 h after transfection, the cell proliferation, invasion, metastasis index and the relative expression levels of LncRNA SNHG14 and MUC4 in the miR-211 group were lower than those in the blank group and the control group (P<0.05), and the expression of miR-211 mRNA The level were higher than that of the blank group (P<0.05), and the difference compared between the blank group and the control group were not statistically significant (P>0.05). Conclusion: Over-expression of miR-211 can inhibit the expression of LncRNA SNHG14, which can inhibit the expression of MUC4, thereby inhibit the proliferation, invasion and metastasis of cervical cancer cells.
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