| 刘冠达,刘锐娜,刘偲翔,李健哲,张小军.金银花提取物对重症急性胰腺炎肺损伤大鼠的保护作用及机制研究[J].,2021,(13):2437-2441 |
| 金银花提取物对重症急性胰腺炎肺损伤大鼠的保护作用及机制研究 |
| Protective Effect and Mechanism Study of Lonicerae Japonica Extract on Lung Injury in Rats with Severe Acute Pancreatitis |
| 投稿时间：2021-01-28 修订日期：2021-02-23 |
| DOI：10.13241/j.cnki.pmb.2021.13.008 |
| 中文关键词: 金银花提取物 重症急性胰腺炎 肺损伤 NF-κB通路 炎症因子 |
| 英文关键词: Honeysuckle extract Severe acute pancreatitis Lung injury NF-kappa B pathway Inflammatory cytokines |
| 基金项目:广西壮族自治区中医药局自筹经费项目（GZZC2019088）；广西中医药大学校级科研项目（2020MS044） |
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| 中文摘要: |
| 摘要 目的：探讨金银花提取物对重症急性胰腺炎肺损伤大鼠的保护作用及其分子机制。方法：选择60只大鼠并将其分为假手术组、模型组、金银花低剂量组、金银花中剂量组和金银花高剂量组。比较各组的肺组织和胰腺组织病理评分。采用全自动血气分析仪检测各组大鼠血氧分压、二氧化碳分压和氧合指数。采用酶联免疫吸附法检测各组炎症因子[白细胞介素（IL）-1、IL-6和肿瘤坏死因子-α（TNF-α）]水平。免疫印迹法检测各组肺组织中NF-κB通路相关蛋白（p-p65、p-IκBα、p65和IκBα蛋白）水平。结果：与假手术组相比，模型组肺组织和胰腺组织病理评分以及二氧化碳分压明显升高（P<0.05）。与模型组相比，金银花各剂量组肺组织和胰腺组织病理评分以及二氧化碳分压明显下降，并且呈剂量依赖性（P<0.05）。与假手术组相比，模型组血氧分压和氧合指数明显下降（P<0.05）。与模型组相比，金银花各剂量组血氧分压和氧合指数明显升高，并且呈剂量依赖性（P<0.05）。与假手术组相比，模型组IL-1、IL-6和TNF-α水平以及p-p65和p-IκBα蛋白水平明显升高（P<0.05）。与模型组相比，金银花各剂量组IL-1、IL-6和TNF-α水平以及p-p65和p-IκBα蛋白水平明显下降，并且呈剂量依赖性（P<0.05）。结论：金银花提取物对大鼠重症急性胰腺炎肺损伤具有一定保护作用，能够升高血氧分压和氧合指数并降低二氧化碳分压，并且其保护作用可能是通过抑制NF-κB通路介导的促炎症因子IL-1、IL-6和TNF-α的产生，缓解炎症性肺损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the protective effect and molecular mechanism of Lonicerae japonica extract on lung injury in rats with severe acute pancreatitis. Methods: 60 rats were selected and divided into sham operation group, model group, low-dose group of Lonicerae japonica, medium-dose group of Lonicerae japonica and high-dose group of Lonicerae japonica. The pathological scores of lung tissue and pancreas tissue were compared in each group. Blood oxygen partial pressure, carbon dioxide partial pressure and oxygenation index of rats in each group were measured by automatic blood gas analyzer. The levels of inflammatory factors [interleukin (IL-1), IL-6 and tumor necrosis factor-α (TNF-α)] in each group were detected by enzyme-linked immunosorbent assay. The protein levels of NF-κB pathway related proteins (p-p65, p-IκBα, p65 and IκBα proteins) in lung tissues in each group were detected by Western blotting. Results: Compared with the sham operation group, the pathological scores of lung tissue and pancreas tissue and the carbon dioxide partial pressure in the model group were significantly increased (P<0.05). Compared with the model group, the pathological scores of lung tissue and pancreas tissue and the carbon dioxide partial pressure in different doses groups of Lonicerae japonica were significantly decreased, and in a dose-dependent manner(P<0.05). Compared with sham operation group, the blood oxygen partial pressure and oxygenation index in model group were significantly decreased(P<0.05). Compared with model group, the blood oxygen partial pressure and oxygenation index in different doses groups of Lonicerae japonica were significantly increased, and in a dose-dependent manner(P<0.05). Compared with sham operation group, the levels of IL-1, IL-6 and TNF-α as well as the protein levels of p-p65 and p-IκBα in model group were significantly increased(P<0.05). Compared with model group, the levels of IL-1, IL-6 and TNF-α as well as the protein levels of p-p65 and p-IκBα in different doses groups of Lonicerae japonica were significantly decreased, and in a dose-dependent manner(P<0.05). Conclusion: Lonicerae japonicae extract has a protective effect on lung injury in rats with severe acute pancreatitis, it can increase blood pressure and oxygenation index and reduce carbon dioxide partial pressure, and its protective effect may be through inhibiting the production of NF-κB pathway mediated pro-inflammatory cytokines IL-1, IL-6 and TNF-α, and alleviate inflammatory lung injury. |
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