| 谭力芯,叶艳艳,熊 宇,税桦桦,黄文静,丁大莲,曾宁碧,赵 希.miRNA-145-5p调控FMNL2基因对口腔鳞癌干细胞增殖、迁移的影响[J].,2021,(12):2221-2225 |
| miRNA-145-5p调控FMNL2基因对口腔鳞癌干细胞增殖、迁移的影响 |
| Effects of miRNA-145-5p Regulating FMNL2 Gene on Proliferation and Migration of Oral Squamous Cell Carcinoma Stem Cells |
| 投稿时间：2020-09-21 修订日期：2020-10-17 |
| DOI：10.13241/j.cnki.pmb.2021.12.005 |
| 中文关键词: miRNA-145-5p 形成素2 口腔鳞癌干细胞 |
| 英文关键词: miRNA-145-5p Morphogen 2 Oral squamous cell carcinoma stem cells |
| 基金项目:教育部留学回国人员科研启动基金项目(HG2015-002)；院级军科基金项目(SWH2016JSTSYB-40N) |
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| 中文摘要: |
| 摘要 目的：分析miRNA-145-5p调控形成素2（formin-like 2，FMNL2）基因对口腔鳞癌干细胞增殖、迁移的影响。方法：按照脂质体2000说明书对细胞进行转染miRNA-145-5p inhibitor及miRNA-145-5p mimics，按照实验设计，将其分为空白组、沉默组（miRNA-145-5p inhibitor）及过表达组（miRNA-145-5p mimics）。荧光定量PCR法检测miRNA-145-5p、FMNL2表达量，MTT检测细胞增殖能力，流式细胞仪检测细胞凋亡能力，细胞划痕实验检测细胞迁移能力，采用Western blot法检测各组细胞中Wnt/β-catenin信号通路蛋白表达量。结果：过表达组miRNA-145-5p、Bax蛋白表达量，凋亡率，细胞G0/G1比例高于沉默组，具有统计学差异（P＜0.05）；过表达组口腔鳞癌干细胞增殖率，FMNL2表达量、MMP-13、β-catenin、Bcl-2、APC蛋白表达量低于沉默组，具有统计学差异（P＜0.05）；过表达组口腔鳞癌干细胞迁移能力弱于沉默组，具有统计学差异（P＜0.05）。结论：miRNA-145-5p通过靶向调控FMNL2，作用于Wnt/β-catenin信号通路调控口腔鳞癌干细胞，进而抑制细胞增殖、迁移。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the effect of miRNA-145-5p regulating homologous morphogen-like protein 2 (FMNL2) gene on the proliferation and migration of oral squamous cell carcinoma stem cells. Methods: Transfect cells with miRNA-145-5p inhibitor and miRNA-145-5p mimics according to the liposome 2000 instructions, and divide them into blank group, silent group (miRNA-145-5p inhibitor) and overexpression group according to the experimental design (MiRNA-145-5p mimics). Fluorescence quantitative PCR method was used to detect the expression of miRNA-145-5p and FMNL2, MTT to detect cell proliferation, flow cytometry to detect cell apoptosis, cell scratch test to detect cell migration ability, Western blot to detect Wnt in each group of cells /β-catenin signaling pathway protein expression. Results: The overexpression group miRNA-145-5p, Bax protein expression, apoptosis rate, cell G0/G1 ratio were higher than the silent group, with statistical differences (P＜0.05); overexpression group oral squamous cell carcinoma stem cell proliferation rate, FMNL2 expression The protein expression levels of MMP-13, β-catenin, Bcl-2, and APC were lower than those in the silent group, with statistical differences(P<0.05); the migration ability of oral squamous cell carcinoma stem cells in the overexpression group was weaker than that in the silent group, with statistical significance Difference(P<0.05). Conclusion: By targeting FMNL2, mirna-145-5p acts on Wnt/β-catenin signaling pathway to regulate the proliferation and migration of OSCC stem cells. |
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