| 陈 艳,梁 奇,杨志武,刘 珊,陈 文.CTLA-4在弥漫大B细胞淋巴瘤患者外泌体的表达及初步机制研究[J].,2021,(7):1396-1400 |
| CTLA-4在弥漫大B细胞淋巴瘤患者外泌体的表达及初步机制研究 |
| Expression of CTLA-4 in Exosomes of Diffuse Large B Cell Lymphoma and Its Primary Mechanism |
| 投稿时间：2020-11-28 修订日期：2020-12-23 |
| DOI：10.13241/j.cnki.pmb.2021.07.043 |
| 中文关键词: 弥漫大B细胞淋巴瘤 CTLA-4 外泌体 肿瘤免疫 |
| 英文关键词: Diffuse large B cell lymphoma CTLA-4 Exosme Tumor immunity |
| 基金项目:湖南省科技创新计划项目（2018SK52204）；国家自然科学基金青年基金项目（31800814） |
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| 中文摘要: |
| 摘要 目的：探讨细胞毒性T淋巴细胞相关抗原4（CTLA-4）在弥漫大B细胞淋巴瘤（DLBCL）患者外泌体的表达及初步机制。方法：2019年6月至2020年11月就诊于本院的DLBCL患者纳入本项研究，分为缓解组和复发组；选取来医院体检的健康志愿者做为对照组；采用试剂盒分离外泌体，CD63抗体包被磁珠结合后，流式细胞术检测CTLA-4+外泌体的比例；流式细胞术检测CD4+T细胞、CD8+T细胞和调节性T细胞（Treg细胞）的比例。结果：相对于对照组，缓解组DLBCL患者CTLA-4+外泌体的比例升高了37.42%；复发组DLBCL患者CTLA-4+外泌体的比例为6.04%，相对于缓解组，差异具有显著的统计学意义；复发组DLBCL患者CD4/CD8+T细胞比值和Treg细胞比例分别为0.85和0.44%，相对于缓解组，差异均具有显著的统计学意义；相关性分析结果显示CTLA-4+外泌体比例与CD4/CD8+T细胞比值呈负相关，而与Treg细胞比例呈正相关。结论：CTLA-4+外泌体比例在DLBCL患者显著升高，且与淋巴瘤的治疗效果和抗肿瘤免疫反应均具有紧密的相关性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) in exosomes of diffuse large B cell lymphoma (DLBCL). Methods: DLBCL patients admitted to our hospital from June 2019 to November 2020 were included in this study, and were divided into remission group and recurrent group. Healthy volunteers who came to the hospital for physical examination were selected as the control group. The exosomes were isolated with the kit, and the ratio of CTLA-4+ exosomes was detected by flow cytometry after the magnetic beads coated with CD63 antibody were bound. The ratios of CD4+T cells, CD8+T cells and regulatory T cells (Treg cells) were measured by flow cytometry. Results: Compared with the control group, the proportion of CTLA-4+ exosomes in remission DLBCL patients increased by 37.42%. The proportion of CTLA-4+ exosomes in recurrent DLBCL patients was 6.04%, and the difference was statistically significant compared with remission group. The CD4/CD8+T cell ratio and Treg cell ratio of DLBCL patients in the recurrent group were 0.85 and 0.44% respectively, and the differences were statistically significant compared with those in the remission group. Correlation analysis showed that the ratio of CTLA-4+ exosomes was negatively correlated with the ratio of CD4/CD8+T cells, but positively correlated with the ratio of Treg cells. Conclusion: CTLA-4+ exosome proportion is significantly increased in DLBCL patients, and it is closely correlated with the treatment effect of lymphoma and anti-tumor immune response. |
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