| 李武超,张 平,王 元,林茂铨,李范珠,朱志红.D-甘露糖修饰黄芩苷阳离子脂质体的制备及其对肺癌A549细胞增殖的抑制作用[J].,2021,(4):625-628 |
| D-甘露糖修饰黄芩苷阳离子脂质体的制备及其对肺癌A549细胞增殖的抑制作用 |
| Preparation of D-mannose Modified Baicalin Cationic Liposome and Its Inhibitory Effect on the Proliferation of Lung Cancer A549 Cells |
| 投稿时间：2020-08-06 修订日期：2020-08-31 |
| DOI：10.13241/j.cnki.pmb.2021.04.005 |
| 中文关键词: 黄芩苷 阳离子脂质体 体外释放 抗肿瘤活性 |
| 英文关键词: Baicalin Cationic liposome In vitro release Antitumor activity |
| 基金项目:国家自然科学基金项目（81873014） |
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| 中文摘要: |
| 摘要 目的：制备一种D-甘露糖修饰的黄芩苷阳离子脂质体[Baicalin cationic liposome，BC-Lipo（+）]，并考察其对肺癌A549细胞增殖的抑制效果。方法：用乙醇注入法制备BC-Lipo（+），并考察其药剂学性质；以肺癌A549细胞为模型，采用MTT法考察其对肿瘤细胞的抑制效果。结果：透射电镜下可见BC-Lipo（+）呈圆形或类圆形，平均粒径（111.3±2.7）nm，Zeta电位（9.6±0.3）mV，包封率（95.4±0.8）%；体外释药行为符合Ritger-Peppas方程（lnQ=0.3497lnt - 1.6611，r=0.9924）； A549细胞的增殖抑制率可达（88.3±5.7）%。结论：处方和制备工艺合理， BC-Lipo（+）包封率较高，粒径分布均匀，带一定的正电荷，具有明显的体外缓释特性，抑制肺癌A549细胞增殖效果显著，为深入研究肺靶向BC纳米脂质体制剂奠定了基础。 |
| 英文摘要: |
| ABSTRACT Objective: A D-mannose-modified baicalin cationic liposome [BC-Lipo (+)] was prepared and its inhibitory effect on the proliferation of lung cancer A549 cells was investigated. Methods: BC-Lipo (+) was prepared by ethanol injection method, and its properties were investigated; MTT assay was used to investigate the inhibitory effect of BC-Lipo (+) on tumor cells of lung cancer A549 cells. Results: The BC-Lipo(+) were round or quasi-round under transmission electron microscopy, the average particle size was (111.3±2.7) nm, Zeta potential was (9.6±0.3) mV, entrapment efficiency was (95.4±0.8)%. The drug release behavior in vitro conformed to the Ritger-Peppas equation (lnQ=0.3497lnt-1.6611, r=0.9924). The proliferation inhibition rate of BC-Lipo (+) against A549 cells was (88.3±5.7)%. Conclusion: The formulation and preparation technology were feasible, and the prepared BC-Lipo (+) had high entrapment efficiency, uniform particle size distribution, positive charge. It had a slow-release characteristic in vitro, and significantly inhibited the proliferation of lung cancer A549 cells in vitro, laying a foundation for further research on lung-targeted BC nanoliposome preparations. |
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