| 吴元元,费素娟,苗 蓓,赵锛活,李 桃.羽扇豆醇介导MDM2-p53通路对胃癌细胞生物学行为的影响[J].,2021,(1):46-49 |
| 羽扇豆醇介导MDM2-p53通路对胃癌细胞生物学行为的影响 |
| Lupeol-mediated MDM2-p53 Pathway Affects on the Biological Behavior of Gastric Cancer Cells and Related Mechanisms |
| 投稿时间：2020-08-05 修订日期：2020-08-27 |
| DOI：10.13241/j.cnki.pmb.2021.01.008 |
| 中文关键词: 羽扇豆醇 MDM2-p53通路 胃癌 细胞增殖 细胞凋亡 |
| 英文关键词: Lupeol MDM2-p53 pathway Gastric cancer Cell proliferation Apoptosis |
| 基金项目:江苏省卫计委医学科研项目（H2018111） |
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| 中文摘要: |
| 摘要 目的：探讨羽扇豆醇介导鼠双微基因2(Mouse double microgene 2，MDM2)-p53通路对胃癌细胞生物学行为的影响及相关机制。方法：对数生长期的胃癌小鼠MFC细胞株随机分为三组。实验1组与实验2组给予10 mg/L和20 mg/L的羽扇豆醇处理，对照组以等体积的1×磷酸盐缓冲液处理。对比三组MFC细胞细胞增殖、凋亡、迁移与侵袭，及MDM2-p53通路蛋白表达。结果：细胞处理后6 h与12 h，实验1组与实验2组的细胞增殖指数、细胞迁移与侵袭指数、MDM2蛋白相对表达水平显著低对于对照组，实验2组也低于实验1组，对比差异都有统计学意义(P＜0.05)。细胞处理后6 h与12 h，实验1组与实验2组的细胞凋亡指数、p53蛋白相对表达水平显著高于对照组，实验2组也高于实验1组，对比差异都有统计学意义(P＜0.05)。结论：羽扇豆醇能促进胃癌细胞p53蛋白的表达，抑制MDM2蛋白的表达，从而促进细胞凋亡，抑制胃癌的增殖、侵袭与转移，且具有剂量依赖性。 |
| 英文摘要: |
| ABSTRACT Objective: To explore Lupeol-mediated mouse double microgene 2 (MDM2)-p53 pathway affects the biological behavior of gastric cancer cells and related mechanisms. Methods: Gastric cancer mouse MFC cell lines in logarithmic growth phase were randomly divided into three groups-control group, experiment 1 group and experiment 2 group. experiment 1 group and experiment 2 group were given 10 mg/L and 20 mg/L lupin alcohol treatment.The control group were treated with an equal volume of 1× phosphate buffer. Compare the cell proliferation, apoptosis, migration and invasion of the three groups of MFC cells, and the expression of MDM2-p53 pathway protein. Results: At 6 h and 12 h after cell treatment, the relative expression levels of cell proliferation index, cell migration and invasion index, and MDM2 protein in experimental group 1 and experimental group 2 were significantly lower than the control group, and the experimental group 2 were also lower than experimental group 1, compared the differences all have statistical significance (P<0.05). At 6 h and 12 h after cell treatment, the relative expression levels of apoptosis index and p53 protein in the experimental group 1 and experimental group 2 were significantly higher than those in the control group, and the experimental group 2 were also higher than that in experimental group 1, compared the differences were statistically significant (P<0.05). Conclusion: Lupeol can promote the expression of p53 protein and inhibit the expression of MDM2 protein in gastric cancer cells, thereby promote apoptosis, inhibiting the proliferation, invasion and metastasis of gastric cancer in dose-dependent manner. |
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